Analysis for variations in the glucose-6-phosphate dehydrogenase gene


Grant Data
Project Title
Analysis for variations in the glucose-6-phosphate dehydrogenase gene
Principal Investigator
Dr Lam, Veronica Min Sien   (Principal investigator)
Co-Investigator(s)
Dr Johnson   (Co-principal investigator)
Duration
16
Start Date
1994-09-01
Completion Date
1995-12-31
Amount
64700
Conference Title
Presentation Title
HKU Project Code
JRS94/02
Grant Type
UK/Hong Kong Joint Research Scheme
Funding Year
1994/1995
Status
Completed
Objectives
To use the Lerman's MELT AND SQHTX programmes to simulate melting profile and electrophoretic behaviour of the DNA fragment(s) to be analysed. It will also assist in the design of the oligonucleotide primers and/or with the necessary GC clamps for DNA amplification in order to resolve all variations in each of the DNA fragments; to develop the denaturing gradient gel electrophoresis in Hong Kong for detecting variations in the G6PD gene; to use the DGGE to detect unequivocally female carrier/heterozygote and identify at risk families; to develop direct sequencing of some of the amplified products so as to identify new mutation(s).