Platform Development of All-optical Laser-scanning Spinning Imaging Assay (ASIA) for Ultralarge-scale Cellular and Tissue Analysis
Grant Data
Project Title
Platform Development of All-optical Laser-scanning Spinning Imaging Assay (ASIA) for Ultralarge-scale Cellular and Tissue Analysis
Principal Investigator
Professor Tsia, Kevin Kin Man
(Project Coordinator (PC))
Co-Investigator(s)
Professor Wong Kenneth Kin Yip
(Co-Investigator)
Dr Hsin Kuan-Yew Michael
(Co-Investigator)
Professor So Hayden Kwok Hay
(Co-Investigator)
Duration
12
Start Date
2019-06-03
Completion Date
2020-06-30
Amount
285180
Conference Title
Platform Development of All-optical Laser-scanning Spinning Imaging Assay (ASIA) for Ultralarge-scale Cellular and Tissue Analysis
Keywords
(ASIA), All-optical Laser-scanning Spinning Imaging Assay, Tissue Analysis, Ultralarge-scale Cellular
Discipline
Others - Electrical and Electronic Engineering
Panel
Engineering (E)
HKU Project Code
InP/113/19
Grant Type
Innovation and Technology Fund Internship Programme
Funding Year
2018
Status
Completed
Objectives
Image-based assay, alongside the advances in automated microscopy and image analysis, presents a powerful tool in direct assessments of biological properties/responses of cells/tissues by visualizing and quantifying their phenotypic profiles. Despite its recognized impact in advancing fundamental biology research and drug discovery, its continuing development is fundamentally hampered by two challenges: (1) lack of throughput to systematically screen a large, heterogeneous cell/tissue population under extensive experimental conditions; (2) overwhelming reliance on biochemical markers, which are not always effective, especially when there is poor prior knowledge of the markers. To address these challenges, we aim to develop a new assay platform, coined All-optical laser-scanning Spinning Imaging Assay (ASIA). Harnessing the high-speed spinning-disk motion, ASIA enables ultralarge-scale, high-resolution ""on-the-fly"" single-cell or whole-tissue-slide imaging on disk at an imaging rate of at least 2 orders-of-magnitude higher than current assays. Not only can ASIA extract the biochemical signatures, but also biophysical phenotypes at single-cell precision. Integrated with high-performance computing, ASIA thus empowers ""deep"" analysis of cells/tissues, especially both the biochemical and biophysical heterogeneity and their underexploited phenotypic correlation. In long term, this high-throughput and high-content technology could open a new paradigm in data-driven bioassay applied in disease diagnostics, and biotechnology industries.