Biomimetic 3D Microsystem to Study Tumour Survival and Drug Responses

Professor Tsao, George Sai Wah   (Co-Principal Investigator (Co-PI) (for projects led by other university))

Professor Pang Stella W.   (Project coordinator)

36

2016-06-01

2019-05-31

800000

Biomimetic 3D Microsystem to Study Tumour Survival and Drug Responses

3D cell culture microsystem, Cell positioning and movement, In vitro tumour model, Nanotechnology, Tumour responses

Others - Electrical and Electronic EngineeringBioelectronics

C1013-15G

Collaborative Research Fund (CRF) - Group Research Project

2015

Completed

1. To characterise the morphometric and physiological parameters of a NPC tumour based on anatomy of tumour biopsies for the design of a 3D biomimetic microsystem. 2. To establish a 3D cell culture microsystem as a realistic mimicry of a tumour, in particular human NPC, based on the morphometric data obtained in Objective 1, with cancer cells and stromal fibroblasts infiltrated by tubules with endothelial cells that resemble blood capillaries, through the development of advanced reversal nanoimprint and nanotechnology, novel materials, sensors, and multiple fluidic control elements with precise positioning and movement of cells, molecules, and chemicals. 3. To validate the 3D microsystem developed in Objective 2 as a biomimetic tumour model by comparing cancer cell growth kinetics obtained from the 3D engineered platform with xenograft NPC tumours and to further optimise the design of the 3D bioreactor. 4. To build quantitative models on tumour survival, proliferation, and drug responses by systematically comparing the effect of cell ratio, supply of oxygen, chemicals, and cytotoxic drug in the 3D microsystem optimised in Objective 3.