Biomimetic 3D Microsystem to Study Tumour Survival and Drug Responses


Grant Data
Project Title
Biomimetic 3D Microsystem to Study Tumour Survival and Drug Responses
Principal Investigator
Professor Wong, Alice Sze Tsai   (Co-principal investigator)
Co-Investigator(s)
Professor Pang Stella W.   (Project coordinator)
Duration
36
Start Date
2016-06-01
Completion Date
2019-05-31
Amount
360000
Conference Title
Presentation Title
Keywords
3D cell culture microsystem, Nanotechnology, Cell positioning and movemnet, In vitro tumour model, Tumour responses
Discipline
Others - Electrical and Electronic Engineering,Bioelectronics
Panel
Engineering (E)
Sponsor
RGC Collaborative Research Fund (CRF)
HKU Project Code
C1013-15G
Grant Type
Collaborative Research Fund (CRF) - Group Research Project
Funding Year
2015/2016
Status
On-going
Objectives
2. To establish a 3D cell culture microsystem as a realistic mimicry of a tumour, in particular human NPC, based on the morphometric data obtained in Objective 1, with cancer cells and stromal fibroblasts infiltrated by tubules with endothelial cells that resemble blood capillaries, through the development of advanced reversal nanoimprint and nanotechnology, novel materials, sensors, and multiple fluidic control elements with precise positioning and movement of cells, molecules, and chemicals. 3. To validate the 3D microsystem developed in Objective 2 as a biomimetic tumour model by comparing cancer cell growth kinetics obtained from the 3D engineered platform with xenograft NPC tumours and to further optimise the design of the 3D bioreactor. 4. To build quantitative models on tumour survival, proliferation, and drug responses by systematically comparing the effect of cell ratio, supply of oxygen, chemicals, and cytotoxic drug in the 3D microsystem optimised in Objective 3.