DNA Display SELEX ??a new approach to evolve fluorescent tools to image RNA in living cells
Grant Data
Project Title
DNA Display SELEX ??a new approach to evolve fluorescent tools to image RNA in living cells
Principal Investigator
Professor Tanner, Julian Alexander
(Principal Investigator (PI))
Co-Investigator(s)
Professor Shum Ho Cheung
(Co-Investigator)
Duration
42
Start Date
2016-01-01
Amount
994480
Conference Title
DNA Display SELEX ??a new approach to evolve fluorescent tools to image RNA in living cells
Presentation Title
Keywords
aptamer, evolution, fluorescence, RNA, selection
Discipline
Molecular Biology
Panel
Biology and Medicine (M)
HKU Project Code
17127515
Grant Type
General Research Fund (GRF)
Funding Year
2015
Status
Completed
Objectives
1 Establish the DNA display technique on beads and investigate integration with emulsion PCR using previously discovered aptamers as a proof-of-principal. 2 Evolve new fluorophore-RNA aptamers with a range of selected fluorescent properties using fluorescence activated cell sorting coupled to deep sequencing. 3 Characterize newly selected fluorophore-RNA aptamers in vitro for biophysical properties, stability, environmental impact on fluorescence, and potential for fusion with longer RNAs. 4 Observe newly selected fluorophore-RNA aptamers expressed in cells using E. coli and human cell culture as two independent model cell systems.