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- Publisher Website: 10.1016/j.aquatox.2007.02.006
- Scopus: eid_2-s2.0-33947586193
- PMID: 17374408
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Article: Induction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus)
Title | Induction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus) |
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Authors | |
Keywords | Apoptosis Caspases Oxidative stress Perfluorinated organic compounds Primary cultured hepatocytes Reactive oxygen species Tilapia |
Issue Date | 2007 |
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox |
Citation | Aquatic Toxicology, 2007, v. 82 n. 2, p. 135-143 How to Cite? |
Abstract | Perfluorinated organic compounds (PFOCs) are emerging persistent organic pollutants (POPs) widely present in the environment, wildlife and human. We studied the cellular toxicology of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) on oxidative stress and induction of apoptosis in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus). Cultured hepatocytes were exposed to PFOS or PFOA (0, 1, 5, 15 and 30 mg L-1) for 24 h, and a dose-dependent decrease in cell viability was determined using trypan blue exclusion method. Significant induction of reactive oxygen species (ROS) accompanied by increases in activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) were found, while activities of glutathione peroxidase (GPx) and glutathione-S-transferase (GST) were decreased. Glutathione (GSH) content was reduced following treatment of PFOA and PFOS. A dose-dependent increase in the lipid peroxidation (LPO) level (measured as maleic dialdehyde, MDA) was observed only in the PFOA exposure groups, whereas LPO remained unchanged in the PFOS exposure groups. Furthermore, a significant activation of caspase-3, -8, -9 activities was evident in both PFOS and PFOA exposure groups. Typical DNA fragmentation (DNA laddering) was further characterized by agarose gel electrophoresis. The overall results demonstrated that PFOS and PFOA are able to produce oxidative stress and induce apoptosis with involvement of caspases in primary cultured tilapia hepatocytes. © 2007 Elsevier B.V. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/92803 |
ISSN | 2023 Impact Factor: 4.1 2023 SCImago Journal Rankings: 1.099 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Liu, C | en_HK |
dc.contributor.author | Yu, K | en_HK |
dc.contributor.author | Shi, X | en_HK |
dc.contributor.author | Wang, J | en_HK |
dc.contributor.author | Lam, PKS | en_HK |
dc.contributor.author | Wu, RSS | en_HK |
dc.contributor.author | Zhou, B | en_HK |
dc.date.accessioned | 2010-09-17T10:57:37Z | - |
dc.date.available | 2010-09-17T10:57:37Z | - |
dc.date.issued | 2007 | en_HK |
dc.identifier.citation | Aquatic Toxicology, 2007, v. 82 n. 2, p. 135-143 | en_HK |
dc.identifier.issn | 0166-445X | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/92803 | - |
dc.description.abstract | Perfluorinated organic compounds (PFOCs) are emerging persistent organic pollutants (POPs) widely present in the environment, wildlife and human. We studied the cellular toxicology of perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) on oxidative stress and induction of apoptosis in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus). Cultured hepatocytes were exposed to PFOS or PFOA (0, 1, 5, 15 and 30 mg L-1) for 24 h, and a dose-dependent decrease in cell viability was determined using trypan blue exclusion method. Significant induction of reactive oxygen species (ROS) accompanied by increases in activities of superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) were found, while activities of glutathione peroxidase (GPx) and glutathione-S-transferase (GST) were decreased. Glutathione (GSH) content was reduced following treatment of PFOA and PFOS. A dose-dependent increase in the lipid peroxidation (LPO) level (measured as maleic dialdehyde, MDA) was observed only in the PFOA exposure groups, whereas LPO remained unchanged in the PFOS exposure groups. Furthermore, a significant activation of caspase-3, -8, -9 activities was evident in both PFOS and PFOA exposure groups. Typical DNA fragmentation (DNA laddering) was further characterized by agarose gel electrophoresis. The overall results demonstrated that PFOS and PFOA are able to produce oxidative stress and induce apoptosis with involvement of caspases in primary cultured tilapia hepatocytes. © 2007 Elsevier B.V. All rights reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/aquatox | en_HK |
dc.relation.ispartof | Aquatic Toxicology | en_HK |
dc.subject | Apoptosis | en_HK |
dc.subject | Caspases | en_HK |
dc.subject | Oxidative stress | en_HK |
dc.subject | Perfluorinated organic compounds | en_HK |
dc.subject | Primary cultured hepatocytes | en_HK |
dc.subject | Reactive oxygen species | en_HK |
dc.subject | Tilapia | en_HK |
dc.title | Induction of oxidative stress and apoptosis by PFOS and PFOA in primary cultured hepatocytes of freshwater tilapia (Oreochromis niloticus) | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Wu, RSS: rudolfwu@hku.hk | en_HK |
dc.identifier.authority | Wu, RSS=rp01398 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.aquatox.2007.02.006 | en_HK |
dc.identifier.pmid | 17374408 | - |
dc.identifier.scopus | eid_2-s2.0-33947586193 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-33947586193&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 82 | en_HK |
dc.identifier.issue | 2 | en_HK |
dc.identifier.spage | 135 | en_HK |
dc.identifier.epage | 143 | en_HK |
dc.identifier.isi | WOS:000245962400006 | - |
dc.publisher.place | Netherlands | en_HK |
dc.identifier.scopusauthorid | Liu, C=8318316300 | en_HK |
dc.identifier.scopusauthorid | Yu, K=35757266000 | en_HK |
dc.identifier.scopusauthorid | Shi, X=16067334700 | en_HK |
dc.identifier.scopusauthorid | Wang, J=8941425500 | en_HK |
dc.identifier.scopusauthorid | Lam, PKS=7202365776 | en_HK |
dc.identifier.scopusauthorid | Wu, RSS=7402945079 | en_HK |
dc.identifier.scopusauthorid | Zhou, B=7401906781 | en_HK |
dc.identifier.issnl | 0166-445X | - |