File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1002/jms.894
- Scopus: eid_2-s2.0-25144454526
- PMID: 16106416
- WOS: WOS:000232078200006
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Performance of the computer algorithm COMPLX for the detection of protein complexes in the mass spectra of simulated biological mixtures
Title | Performance of the computer algorithm COMPLX for the detection of protein complexes in the mass spectra of simulated biological mixtures |
---|---|
Authors | |
Keywords | Protein complex Algorithm Biological mixtures COMPLX Mass spectrometry |
Issue Date | 2005 |
Citation | Journal of Mass Spectrometry, 2005, v. 40, n. 9, p. 1187-1196 How to Cite? |
Abstract | The performance of the algorithm COMPLX for detecting protein-ligand or other macromolecular complexes has been tested for highly complex data sets. These data contain m/z values for ions of proteins of the SWISS-PROT database within simulated biological mixtures where each component shares a similar molecular weight and/or isoelectric point (pi). As many as 1600 ion signals were entered to challenge the algorithm to identify ion signals associated with a single protein complex that has been ionised and detected within a mass spectrometer. Despite the complexity of such data sets, the algorithm is shown to be able to identify the presence of individual bimolecular complexes. The output data can be re-evaluated by the user as necessary in light of any additional information that is known concerning the nature of predicted associations, as well as the quality of the data-set in terms of errors in m/z values as a direct consequence of the mass calibration or resolution achieved. The data presented illustrates that the best results are obtained when output results are ranked according to the largest continuous series of ion pairs detected for a protein or macromolecule and its complex for which the ligand mass is assigned the lowest mass error. Copyright © 2005 John Wiley & Sons, Ltd. |
Persistent Identifier | http://hdl.handle.net/10722/250901 |
ISSN | 2023 Impact Factor: 1.9 2023 SCImago Journal Rankings: 0.431 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wong, Jason W.H. | - |
dc.contributor.author | Downard, Kevin M. | - |
dc.date.accessioned | 2018-02-01T01:54:02Z | - |
dc.date.available | 2018-02-01T01:54:02Z | - |
dc.date.issued | 2005 | - |
dc.identifier.citation | Journal of Mass Spectrometry, 2005, v. 40, n. 9, p. 1187-1196 | - |
dc.identifier.issn | 1076-5174 | - |
dc.identifier.uri | http://hdl.handle.net/10722/250901 | - |
dc.description.abstract | The performance of the algorithm COMPLX for detecting protein-ligand or other macromolecular complexes has been tested for highly complex data sets. These data contain m/z values for ions of proteins of the SWISS-PROT database within simulated biological mixtures where each component shares a similar molecular weight and/or isoelectric point (pi). As many as 1600 ion signals were entered to challenge the algorithm to identify ion signals associated with a single protein complex that has been ionised and detected within a mass spectrometer. Despite the complexity of such data sets, the algorithm is shown to be able to identify the presence of individual bimolecular complexes. The output data can be re-evaluated by the user as necessary in light of any additional information that is known concerning the nature of predicted associations, as well as the quality of the data-set in terms of errors in m/z values as a direct consequence of the mass calibration or resolution achieved. The data presented illustrates that the best results are obtained when output results are ranked according to the largest continuous series of ion pairs detected for a protein or macromolecule and its complex for which the ligand mass is assigned the lowest mass error. Copyright © 2005 John Wiley & Sons, Ltd. | - |
dc.language | eng | - |
dc.relation.ispartof | Journal of Mass Spectrometry | - |
dc.subject | Protein complex | - |
dc.subject | Algorithm | - |
dc.subject | Biological mixtures | - |
dc.subject | COMPLX | - |
dc.subject | Mass spectrometry | - |
dc.title | Performance of the computer algorithm COMPLX for the detection of protein complexes in the mass spectra of simulated biological mixtures | - |
dc.type | Article | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1002/jms.894 | - |
dc.identifier.pmid | 16106416 | - |
dc.identifier.scopus | eid_2-s2.0-25144454526 | - |
dc.identifier.volume | 40 | - |
dc.identifier.issue | 9 | - |
dc.identifier.spage | 1187 | - |
dc.identifier.epage | 1196 | - |
dc.identifier.isi | WOS:000232078200006 | - |
dc.identifier.issnl | 1076-5174 | - |