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- Publisher Website: 10.1093/chromsci/47.5.337
- Scopus: eid_2-s2.0-66749178871
- PMID: 19476698
- WOS: WOS:000273676800004
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Article: Separation and purification of epimedin A, B, C, and icariin from the medicinal herb epimedium brevicornum maxim by dual-mode HSCCC
Title | Separation and purification of epimedin A, B, C, and icariin from the medicinal herb epimedium brevicornum maxim by dual-mode HSCCC |
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Authors | |
Issue Date | 2009 |
Publisher | Preston Publications, Inc. The Journal's web site is located at http://www.j-chrom-sci.com/ |
Citation | Journal Of Chromatographic Science, 2009, v. 47 n. 5, p. 337-340 How to Cite? |
Abstract | Epimedium brevicornum Maxim is a famous medicinal herb which has been widely used for the treatment of impotence, infertility, osteoporosis, cardiovascular diseases, amnesia, and senile functional diseases. A dual-mode high-speed counter-current chromatographic method is developed for the separation and purification of four bioactive flavonoids from the medicinal herb Epimedium brevicornum Maxim. The crude flavonoids are obtained by extraction with ethyl acetate and ethanol from the dried aerial parts of Epimedium brevicornum Maxim under sonication. Highspeed counter-current chromatography with a two-phase solvent system composed of n-butanol-ethyl acetate-water (3:7:10, v/v) is performed using a dual-mode method, which yields 3.2 mg of epimedin A at a purity of 98.2%, 5.5 mg of epimedin B at a purity of 92.6%, 12.7 mg of epimedin C at a purity of 90.4%, and 42.9 mg of icariin at a purity of 96.8% based on the HPLC analysis. The recoveries are 95.2%, 89.4%, 91.1%, and 94.8%, respectively. The isolation of these flavonoids in adequate amounts makes them readily available for structure-activity relationship studies and for quality control of the herbal medicine. |
Persistent Identifier | http://hdl.handle.net/10722/179135 |
ISSN | 2023 Impact Factor: 1.5 2023 SCImago Journal Rankings: 0.342 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Li, HB | en_US |
dc.contributor.author | Chen, F | en_US |
dc.date.accessioned | 2012-12-19T09:52:15Z | - |
dc.date.available | 2012-12-19T09:52:15Z | - |
dc.date.issued | 2009 | en_US |
dc.identifier.citation | Journal Of Chromatographic Science, 2009, v. 47 n. 5, p. 337-340 | en_US |
dc.identifier.issn | 0021-9665 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179135 | - |
dc.description.abstract | Epimedium brevicornum Maxim is a famous medicinal herb which has been widely used for the treatment of impotence, infertility, osteoporosis, cardiovascular diseases, amnesia, and senile functional diseases. A dual-mode high-speed counter-current chromatographic method is developed for the separation and purification of four bioactive flavonoids from the medicinal herb Epimedium brevicornum Maxim. The crude flavonoids are obtained by extraction with ethyl acetate and ethanol from the dried aerial parts of Epimedium brevicornum Maxim under sonication. Highspeed counter-current chromatography with a two-phase solvent system composed of n-butanol-ethyl acetate-water (3:7:10, v/v) is performed using a dual-mode method, which yields 3.2 mg of epimedin A at a purity of 98.2%, 5.5 mg of epimedin B at a purity of 92.6%, 12.7 mg of epimedin C at a purity of 90.4%, and 42.9 mg of icariin at a purity of 96.8% based on the HPLC analysis. The recoveries are 95.2%, 89.4%, 91.1%, and 94.8%, respectively. The isolation of these flavonoids in adequate amounts makes them readily available for structure-activity relationship studies and for quality control of the herbal medicine. | en_US |
dc.language | eng | en_US |
dc.publisher | Preston Publications, Inc. The Journal's web site is located at http://www.j-chrom-sci.com/ | en_US |
dc.relation.ispartof | Journal of Chromatographic Science | en_US |
dc.subject.mesh | Chromatography, High Pressure Liquid | en_US |
dc.subject.mesh | Countercurrent Distribution - Methods | en_US |
dc.subject.mesh | Epimedium - Chemistry | en_US |
dc.subject.mesh | Flavonoids - Isolation & Purification | en_US |
dc.title | Separation and purification of epimedin A, B, C, and icariin from the medicinal herb epimedium brevicornum maxim by dual-mode HSCCC | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_US |
dc.identifier.authority | Chen, F=rp00672 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1093/chromsci/47.5.337 | - |
dc.identifier.pmid | 19476698 | - |
dc.identifier.scopus | eid_2-s2.0-66749178871 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-66749178871&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 47 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.spage | 337 | en_US |
dc.identifier.epage | 340 | en_US |
dc.identifier.isi | WOS:000273676800004 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Li, HB=25649944400 | en_US |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_US |
dc.identifier.issnl | 0021-9665 | - |