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- Publisher Website: 10.1002/jssc.200400002
- Scopus: eid_2-s2.0-15944373151
- PMID: 15776929
- WOS: WOS:000227397400008
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Article: Simultaneous separation and purification of five bioactive coumarins from the Chinese medicinal plant Cnidium monnieri by high-speed counter-current chromatography
Title | Simultaneous separation and purification of five bioactive coumarins from the Chinese medicinal plant Cnidium monnieri by high-speed counter-current chromatography |
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Authors | |
Keywords | Cnidium monnieri Coumarin Counter-current chromatography |
Issue Date | 2005 |
Publisher | Wiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/jss |
Citation | Journal Of Separation Science, 2005, v. 28 n. 3, p. 268-272 How to Cite? |
Abstract | Cnidium monnieri (L.) Cusson is a well-known Chinese medicinal plant, which has been used for the treatment of impotence, frigidity, and skin-related diseases, and exhibits strong antipruritic, antiallergic, antidermatophytic, antibacterial, antifungal, and antiosteoporotic activities. A high-speed counter-current chromatography method was developed for the separation and purification of five bioactive coumarins from this plant. The crude coumarins were obtained by ethanol extraction from the dried fruits of Cnidium monnieri (L.) Cusson under sonication. High-speed counter-current chromatography with the two-phase solvent systems n-hexane-ethyl acetate-ethanol-water (5:5:4:6, v/v) and n-hexane-ethyl acetate-ethanol-water (5:5:6:4, v/v) was successfully performed with stepwise elution. The five relatively pure coumarins were obtained from 500 mg of the crude extract in a single run. Their purities were 90.6-98.9%, and the recoveries were 85.7-94.2%. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Persistent Identifier | http://hdl.handle.net/10722/178867 |
ISSN | 2023 Impact Factor: 2.8 2023 SCImago Journal Rankings: 0.533 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
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dc.contributor.author | Li, HB | en_US |
dc.contributor.author | Chen, F | en_US |
dc.date.accessioned | 2012-12-19T09:50:16Z | - |
dc.date.available | 2012-12-19T09:50:16Z | - |
dc.date.issued | 2005 | en_US |
dc.identifier.citation | Journal Of Separation Science, 2005, v. 28 n. 3, p. 268-272 | en_US |
dc.identifier.issn | 1615-9306 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178867 | - |
dc.description.abstract | Cnidium monnieri (L.) Cusson is a well-known Chinese medicinal plant, which has been used for the treatment of impotence, frigidity, and skin-related diseases, and exhibits strong antipruritic, antiallergic, antidermatophytic, antibacterial, antifungal, and antiosteoporotic activities. A high-speed counter-current chromatography method was developed for the separation and purification of five bioactive coumarins from this plant. The crude coumarins were obtained by ethanol extraction from the dried fruits of Cnidium monnieri (L.) Cusson under sonication. High-speed counter-current chromatography with the two-phase solvent systems n-hexane-ethyl acetate-ethanol-water (5:5:4:6, v/v) and n-hexane-ethyl acetate-ethanol-water (5:5:6:4, v/v) was successfully performed with stepwise elution. The five relatively pure coumarins were obtained from 500 mg of the crude extract in a single run. Their purities were 90.6-98.9%, and the recoveries were 85.7-94.2%. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. | en_US |
dc.language | eng | en_US |
dc.publisher | Wiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/jss | en_US |
dc.relation.ispartof | Journal of Separation Science | en_US |
dc.subject | Cnidium monnieri | - |
dc.subject | Coumarin | - |
dc.subject | Counter-current chromatography | - |
dc.subject.mesh | Chromatography, High Pressure Liquid | en_US |
dc.subject.mesh | Cnidium - Chemistry | en_US |
dc.subject.mesh | Coumarins - Chemistry - Isolation & Purification | en_US |
dc.subject.mesh | Drugs, Chinese Herbal - Chemistry - Isolation & Purification | en_US |
dc.subject.mesh | Molecular Structure | en_US |
dc.subject.mesh | Plant Extracts - Chemistry | en_US |
dc.subject.mesh | Plants, Medicinal - Chemistry | en_US |
dc.subject.mesh | Solvents | en_US |
dc.title | Simultaneous separation and purification of five bioactive coumarins from the Chinese medicinal plant Cnidium monnieri by high-speed counter-current chromatography | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_US |
dc.identifier.authority | Chen, F=rp00672 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1002/jssc.200400002 | en_US |
dc.identifier.pmid | 15776929 | - |
dc.identifier.scopus | eid_2-s2.0-15944373151 | en_US |
dc.identifier.hkuros | 104953 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-15944373151&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 28 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 268 | en_US |
dc.identifier.epage | 272 | en_US |
dc.identifier.isi | WOS:000227397400008 | - |
dc.publisher.place | Germany | en_US |
dc.identifier.scopusauthorid | Li, HB=25649944400 | en_US |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_US |
dc.identifier.issnl | 1615-9306 | - |