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- Publisher Website: 10.1007/BF02492256
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Article: Simultaneous determination of twelve water- and fat-soluble vitamins by high-performance liquid chromatography with diode array detection
Title | Simultaneous determination of twelve water- and fat-soluble vitamins by high-performance liquid chromatography with diode array detection |
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Authors | |
Keywords | Column Liquid Chromatography Pharmaceutical Preparations Water- And Fat-Soluble Vitamins |
Issue Date | 2001 |
Publisher | Friedr Vieweg und Sohn Verlagsgesellschaft mbH. The Journal's web site is located at http://link.springer.com/journal/10337 |
Citation | Chromatographia, 2001, v. 54 n. 3-4, p. 270-273 How to Cite? |
Abstract | A novel method for the simultaneous determination of twelve water- and fat-soluble vitamins has been established by high-performance liquid chromatography with diode array detection. The vitamins were analyzed on a μBondapak C18 column (300 × 3.9 mm, 10 μm) with methanol- KH2PO4 buffer (0.1 M, pH 7.0)- water as mobile phase in a gradient. The linearity of calibration graphs was compound-dependent and the detection limits ranged from 0.02 μg mL-1 to 0.5 μg mL-1. The method was successfully applied to determine vitamins in pharmaceutical preparations. The recoveries were from 95.1% to 103% and the relative standard deviations were in the range of 0.9% to 4.5%. |
Persistent Identifier | http://hdl.handle.net/10722/178729 |
ISSN | 2023 Impact Factor: 1.2 2023 SCImago Journal Rankings: 0.301 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Li, HB | en_US |
dc.contributor.author | Chen, F | en_US |
dc.date.accessioned | 2012-12-19T09:49:22Z | - |
dc.date.available | 2012-12-19T09:49:22Z | - |
dc.date.issued | 2001 | en_US |
dc.identifier.citation | Chromatographia, 2001, v. 54 n. 3-4, p. 270-273 | en_US |
dc.identifier.issn | 0009-5893 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178729 | - |
dc.description.abstract | A novel method for the simultaneous determination of twelve water- and fat-soluble vitamins has been established by high-performance liquid chromatography with diode array detection. The vitamins were analyzed on a μBondapak C18 column (300 × 3.9 mm, 10 μm) with methanol- KH2PO4 buffer (0.1 M, pH 7.0)- water as mobile phase in a gradient. The linearity of calibration graphs was compound-dependent and the detection limits ranged from 0.02 μg mL-1 to 0.5 μg mL-1. The method was successfully applied to determine vitamins in pharmaceutical preparations. The recoveries were from 95.1% to 103% and the relative standard deviations were in the range of 0.9% to 4.5%. | en_US |
dc.language | eng | en_US |
dc.publisher | Friedr Vieweg und Sohn Verlagsgesellschaft mbH. The Journal's web site is located at http://link.springer.com/journal/10337 | en_US |
dc.relation.ispartof | Chromatographia | en_US |
dc.subject | Column Liquid Chromatography | en_US |
dc.subject | Pharmaceutical Preparations | en_US |
dc.subject | Water- And Fat-Soluble Vitamins | en_US |
dc.title | Simultaneous determination of twelve water- and fat-soluble vitamins by high-performance liquid chromatography with diode array detection | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_US |
dc.identifier.authority | Chen, F=rp00672 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1007/BF02492256 | - |
dc.identifier.scopus | eid_2-s2.0-0034868633 | en_US |
dc.identifier.hkuros | 72650 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0034868633&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 54 | en_US |
dc.identifier.issue | 3-4 | en_US |
dc.identifier.spage | 270 | en_US |
dc.identifier.epage | 273 | en_US |
dc.identifier.isi | WOS:000170734900023 | - |
dc.publisher.place | Germany | en_US |
dc.identifier.scopusauthorid | Li, HB=25649944400 | en_US |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_US |
dc.identifier.issnl | 0009-5893 | - |