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- Publisher Website: 10.1111/j.1365-263X.2011.01207.x
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- PMID: 22211704
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Article: Novel and conventional assays in determining abundance of Streptococcus mutans in saliva
Title | Novel and conventional assays in determining abundance of Streptococcus mutans in saliva |
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Authors | |
Issue Date | 2012 |
Publisher | Wiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0960-7439 |
Citation | International Journal of Paediatric Dentistry, 2012, v. 22 n. 5, p. 363-368 How to Cite? |
Abstract | BACKGROUND: Effective caries control and management requires identification of susceptible children for timely intervention. Streptococcus mutans (S. mutans) is an important biomarker of caries risk. AIM: This study aimed to comparatively evaluate the validities of a novel immunoassay and a conventional culture-based assay in detecting salivary S. mutans in a paediatric cohort. METHODS: 190 children aged 3-4 years were recruited. The abundance of S. mutans in their saliva samples was analysed with three assay systems viz. a conventional culture-based assay (Dentocult SM), a novel immunoassay system (Saliva-Check MUTANS) based on monoclonal antibody technology and a Taqman real-time PCR assay taken as a gold standard. RESULTS: The novel immunoassay accurately differentiated saliva samples with high (>/=5 x 10(5) CFU/mL) and low (<5 x 10(5) CFU/mL) S. mutans levels. The sensitivity/specificity was 97.6%/90.6%. The conventional culture-based assay reached a reasonably high sensitivity/specificity (92.8%/81.3%) in identifying children with moderate (>/=10(4) CFU/mL) S. mutans level. Its sensitivity/ specificity in selecting children with high (>/=10(5) CFU/mL) and very high (>10(6) CFU/mL) S. mutans levels were not sufficient (78.7%/79.8% and 25.8%/91.8%, respectively). CONCLUSION: The monoclonal antibody-based immunoassay accurately and rapidly determines S. mutans abundance in saliva and could be useful for chairside assessment of children's caries risk. |
Persistent Identifier | http://hdl.handle.net/10722/163747 |
ISSN | 2023 Impact Factor: 2.3 2023 SCImago Journal Rankings: 0.885 |
ISI Accession Number ID |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gao, XL | en_US |
dc.contributor.author | Seneviratne, CJ | en_US |
dc.contributor.author | Lo, ECM | en_US |
dc.contributor.author | Chu, CH | en_US |
dc.contributor.author | Samaranayake, LP | en_US |
dc.date.accessioned | 2012-09-20T07:50:38Z | - |
dc.date.available | 2012-09-20T07:50:38Z | - |
dc.date.issued | 2012 | en_US |
dc.identifier.citation | International Journal of Paediatric Dentistry, 2012, v. 22 n. 5, p. 363-368 | en_US |
dc.identifier.issn | 0960-7439 | - |
dc.identifier.uri | http://hdl.handle.net/10722/163747 | - |
dc.description.abstract | BACKGROUND: Effective caries control and management requires identification of susceptible children for timely intervention. Streptococcus mutans (S. mutans) is an important biomarker of caries risk. AIM: This study aimed to comparatively evaluate the validities of a novel immunoassay and a conventional culture-based assay in detecting salivary S. mutans in a paediatric cohort. METHODS: 190 children aged 3-4 years were recruited. The abundance of S. mutans in their saliva samples was analysed with three assay systems viz. a conventional culture-based assay (Dentocult SM), a novel immunoassay system (Saliva-Check MUTANS) based on monoclonal antibody technology and a Taqman real-time PCR assay taken as a gold standard. RESULTS: The novel immunoassay accurately differentiated saliva samples with high (>/=5 x 10(5) CFU/mL) and low (<5 x 10(5) CFU/mL) S. mutans levels. The sensitivity/specificity was 97.6%/90.6%. The conventional culture-based assay reached a reasonably high sensitivity/specificity (92.8%/81.3%) in identifying children with moderate (>/=10(4) CFU/mL) S. mutans level. Its sensitivity/ specificity in selecting children with high (>/=10(5) CFU/mL) and very high (>10(6) CFU/mL) S. mutans levels were not sufficient (78.7%/79.8% and 25.8%/91.8%, respectively). CONCLUSION: The monoclonal antibody-based immunoassay accurately and rapidly determines S. mutans abundance in saliva and could be useful for chairside assessment of children's caries risk. | - |
dc.language | eng | en_US |
dc.publisher | Wiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0960-7439 | - |
dc.relation.ispartof | International Journal of Paediatric Dentistry | en_US |
dc.rights | This is the peer reviewed version of the following article: International Journal of Paediatric Dentistry, 2012, v. 22 n. 5, p. 363-368, which has been published in final form at https://doi.org/10.1111/j.1365-263X.2011.01207.x. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. | - |
dc.title | Novel and conventional assays in determining abundance of Streptococcus mutans in saliva | en_US |
dc.type | Article | en_US |
dc.identifier.email | Gao, XL: gaoxl@hkucc.hku.hk | en_US |
dc.identifier.email | Seneviratne, CJ: jaya@hku.hk | en_US |
dc.identifier.email | Lo, ECM: hrdplcm@hkucc.hku.hk | en_US |
dc.identifier.email | Chu, CH: chchu@hku.hk | en_US |
dc.identifier.email | Samaranayake, LP: lakshman@hku.hk | en_US |
dc.identifier.authority | Gao, X=rp01509 | en_US |
dc.identifier.authority | Seneviratne, CJ=rp01372 | en_US |
dc.identifier.authority | Lo, ECM=rp00015 | en_US |
dc.identifier.authority | Chu, CH=rp00022 | en_US |
dc.identifier.authority | Samaranayake, LP=rp00023 | en_US |
dc.description.nature | postprint | - |
dc.identifier.doi | 10.1111/j.1365-263X.2011.01207.x | - |
dc.identifier.pmid | 22211704 | - |
dc.identifier.scopus | eid_2-s2.0-84864946276 | - |
dc.identifier.hkuros | 210380 | en_US |
dc.identifier.volume | 22 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.spage | 363 | en_US |
dc.identifier.epage | 368 | en_US |
dc.identifier.eissn | 1365-263X | - |
dc.identifier.isi | WOS:000307308900008 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 0960-7439 | - |