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- Publisher Website: 10.1021/ja900543y
- Scopus: eid_2-s2.0-67651221840
- PMID: 19621959
- WOS: WOS:000268395000050
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Article: Structure of a nickel chaperone, HypA, from Helicobacter pylori reveals two distinct metal binding sites
Title | Structure of a nickel chaperone, HypA, from Helicobacter pylori reveals two distinct metal binding sites | ||||||||||
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Authors | |||||||||||
Issue Date | 2009 | ||||||||||
Publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/jacsat/index.html | ||||||||||
Citation | Journal Of The American Chemical Society, 2009, v. 131 n. 29, p. 10031-10040 How to Cite? | ||||||||||
Abstract | Metallochaperones bind metals and ensure the safe delivery of metals to the targets. They are required for the activation and maturation of nickel-containing enzymes [Ni,Fe]-hydrogenase and urease. Metallochaperone HypA was found to be essential to facilitate nickel delivery to hydrogenase together with its partner HypB, although the detailed mechanism is not clear. In this study, we have cloned hypA gene from Helicobacter pylori (strain 26695), overexpressed, and purified the protein. The zinc-bound HypA (Zn-HypA) exists as a monomer in solution, and its solution structure was determined by NMR spectroscopy together with molecular dynamics simulated annealing. Zn-HypA folds into two domains, including a zinc domain and a nickel domain with a mixed α/β structure. The former houses a rigid zinc-binding site possibly with the role of structural stabilization, whereas the latter harbors a nickel-binding site at the N-terminus. Zinc binds to the four conserved cysteines tetrahedrally as evidenced by 113Cd NMR spectroscopy, and nickel coordinates with four nitrogens of the protein probably in a square-planar geometry. Low coordination number of Ni2+ may allow the metal to be readily transferred to its downstream receptors. Our studies may shed light on how the metallochaperone exerts its functions in intracellular nickel delivery. © 2009 American Chemical Society. | ||||||||||
Persistent Identifier | http://hdl.handle.net/10722/157551 | ||||||||||
ISSN | 2023 Impact Factor: 14.4 2023 SCImago Journal Rankings: 5.489 | ||||||||||
ISI Accession Number ID |
Funding Information: We thank Research Grants Council of Hong Kong (HKU7512/05M, HKU7042/07P, HKU7038/08P), RGC Collaborative Research Fund (HKU1/07C and HKU2/06C), the UGC of Hong Kong SAR of China under the scheme of the Area of Excellence, and the University of Hong Kong for their support. We are grateful to Prof. John H. Viles (QMW, London) and Prof. Sunney Chan (Caltech) for helpful discussion and comments. | ||||||||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Xia, W | en_US |
dc.contributor.author | Li, H | en_US |
dc.contributor.author | Sze, KH | en_US |
dc.contributor.author | Sun, H | en_US |
dc.date.accessioned | 2012-08-08T08:51:11Z | - |
dc.date.available | 2012-08-08T08:51:11Z | - |
dc.date.issued | 2009 | en_US |
dc.identifier.citation | Journal Of The American Chemical Society, 2009, v. 131 n. 29, p. 10031-10040 | en_US |
dc.identifier.issn | 0002-7863 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/157551 | - |
dc.description.abstract | Metallochaperones bind metals and ensure the safe delivery of metals to the targets. They are required for the activation and maturation of nickel-containing enzymes [Ni,Fe]-hydrogenase and urease. Metallochaperone HypA was found to be essential to facilitate nickel delivery to hydrogenase together with its partner HypB, although the detailed mechanism is not clear. In this study, we have cloned hypA gene from Helicobacter pylori (strain 26695), overexpressed, and purified the protein. The zinc-bound HypA (Zn-HypA) exists as a monomer in solution, and its solution structure was determined by NMR spectroscopy together with molecular dynamics simulated annealing. Zn-HypA folds into two domains, including a zinc domain and a nickel domain with a mixed α/β structure. The former houses a rigid zinc-binding site possibly with the role of structural stabilization, whereas the latter harbors a nickel-binding site at the N-terminus. Zinc binds to the four conserved cysteines tetrahedrally as evidenced by 113Cd NMR spectroscopy, and nickel coordinates with four nitrogens of the protein probably in a square-planar geometry. Low coordination number of Ni2+ may allow the metal to be readily transferred to its downstream receptors. Our studies may shed light on how the metallochaperone exerts its functions in intracellular nickel delivery. © 2009 American Chemical Society. | en_US |
dc.language | eng | en_US |
dc.publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/jacsat/index.html | en_US |
dc.relation.ispartof | Journal of the American Chemical Society | en_US |
dc.subject.mesh | Bacterial Proteins - Chemistry - Genetics | en_US |
dc.subject.mesh | Binding Sites | en_US |
dc.subject.mesh | Computer Simulation | en_US |
dc.subject.mesh | Helicobacter Pylori - Chemistry - Genetics | en_US |
dc.subject.mesh | Magnetic Resonance Spectroscopy | en_US |
dc.subject.mesh | Metalloproteins - Chemistry - Genetics | en_US |
dc.subject.mesh | Models, Chemical | en_US |
dc.subject.mesh | Models, Molecular | en_US |
dc.subject.mesh | Nickel - Chemistry | en_US |
dc.subject.mesh | Protein Structure, Secondary | en_US |
dc.subject.mesh | Reproducibility Of Results | en_US |
dc.title | Structure of a nickel chaperone, HypA, from Helicobacter pylori reveals two distinct metal binding sites | en_US |
dc.type | Article | en_US |
dc.identifier.email | Sze, KH:khsze@hku.hk | en_US |
dc.identifier.email | Sun, H:hsun@hkucc.hku.hk | en_US |
dc.identifier.authority | Sze, KH=rp00785 | en_US |
dc.identifier.authority | Sun, H=rp00777 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1021/ja900543y | en_US |
dc.identifier.pmid | 19621959 | - |
dc.identifier.scopus | eid_2-s2.0-67651221840 | en_US |
dc.identifier.hkuros | 163891 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-67651221840&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 131 | en_US |
dc.identifier.issue | 29 | en_US |
dc.identifier.spage | 10031 | en_US |
dc.identifier.epage | 10040 | en_US |
dc.identifier.eissn | 1520-5126 | - |
dc.identifier.isi | WOS:000268395000050 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Xia, W=40262950100 | en_US |
dc.identifier.scopusauthorid | Li, H=37063577200 | en_US |
dc.identifier.scopusauthorid | Sze, KH=7006735061 | en_US |
dc.identifier.scopusauthorid | Sun, H=7404827446 | en_US |
dc.identifier.citeulike | 5343190 | - |
dc.identifier.issnl | 0002-7863 | - |