Clinical Translational Research of Non-invasive Embryo Viability Test (niEVT)
Grant Data
Project Title
Clinical Translational Research of Non-invasive Embryo Viability Test (niEVT)
Principal Investigator
Dr Lee, Calvin Kai Fai
(Principal Investigator (PI))
Duration
22
Start Date
2023-10-05
Amount
1017193
Conference Title
Clinical Translational Research of Non-invasive Embryo Viability Test (niEVT)
Keywords
Clinical Translational Research, Non-invasive Embryo Viability Test ,niEVT
Discipline
Medicine, Dentistry and Health
HKU Project Code
PiH/477/23
Grant Type
Research Talent Hub for ITF Projects (RTH-ITF)
Status
On-going
Objectives
This study aims to develop a non-invasive embryo development potential testing kit (niEVT) based on the testing of a spent embryo culture medium. We plan to determine the clinical application value of the team's first-generation kit through a multi-center randomized controlled study. In addition, the second-generation kit will use direct capture, ligation, and detection of cell-free DNA (cfDNA) technology to avoid using single-cell amplification technology. We will also develop technology for detecting microRNA (miRNA) and cfDNA methylation in minute volume of spent embryo culture medium and correlate the identified miRNA and the methylation sites with embryo implantation and pregnancy outcomes of the patients. The research is expected to develop a new non-invasive and objective embryo selection method to improve the success rate of assisted reproductive treatment.