From Structural to Genomic Biology: A Multi-scale Approach Tightly Integrating Structural Biology and Novel Genomics Platform to Quantitatively Analyze the Transcription and its Regulation of SARS-Co V-2 Viral RNA Polymerase
Grant Data
Project Title
From Structural to Genomic Biology: A Multi-scale Approach Tightly Integrating Structural Biology and Novel Genomics Platform to Quantitatively Analyze the Transcription and its Regulation of SARS-Co V-2 Viral RNA Polymerase
Principal Investigator
Professor Li, Xuechen
(Principal Investigator (PI))
Duration
36
Start Date
2022-06-30
Amount
300000
Conference Title
From Structural to Genomic Biology: A Multi-scale Approach Tightly Integrating Structural Biology and Novel Genomics Platform to Quantitatively Analyze the Transcription and its Regulation of SARS-Co V-2 Viral RNA Polymerase
Keywords
1) Viral Polymerase Chemical Biology
2) Nascent RNA Sequencing 3) Cryo-EM Structural Biology 4) 5) Virology
Discipline
Chemical Sciences
Panel
Physical Sciences (P)
HKU Project Code
C6036-21G
Grant Type
Collaborative Research Fund (CRF) - Group Research Project 2021/2022
Funding Year
2021
Status
On-going
Objectives
Aim 1: Mapping genome-wide distribution of transcriptionally engaged SARS-CoV-2 polymerase at base-pair resolution using our novel nascent RNA sequencing to study transcriptional regulation Aim 2: Identification and synthesis of novel NTP analogues that inhibit RNA elongation and escape proofreading by rational designAim 3: Structural elucidation of the molecular mechanisms of elongation and proofreading during viral RNA synthesis.