Development of Cysteine/Penicillamine ligation (CPL) and further explorations
Grant Data
Project Title
Development of Cysteine/Penicillamine ligation (CPL) and further explorations
Principal Investigator
Professor Li, Xuechen
(Principal Investigator (PI))
Duration
36
Start Date
2022-01-01
Amount
666015
Conference Title
Development of Cysteine/Penicillamine ligation (CPL) and further explorations
Presentation Title
Keywords
chemical protein synthesis, peptide ligation, thiolated amino acid
Discipline
Chemical Sciences
Panel
Physical Sciences (P)
HKU Project Code
17302621
Grant Type
General Research Fund (GRF)
Funding Year
2021
Status
On-going
Objectives
1 CPL for selective desulfurization. We aim to develop a strategy for selective desulfurization for chemical protein synthesis 2 CPL with thiolated amino acid and selenocysteine. Usage of the thiolated amino acids and selenocysteine will be explored for CPL. 3 CPL-solubilizing tags. We aim to introduce solubilizing tags via the salicylaldehyde ester moiety to realize CPL-solubilizing modification 4 Long-range thiol participation pathway analogous to CPL. Based on the CPL working principle, we will explore several strategies, including a relayed Lys acylation, an auxiliary-based ligation and intermolecular thiol-assisted peptide cyclization.