Define the core regulomes underlying Hirschsprung pathogenesis using single-cell transcriptomic analysis of human iPSC-derived enteric neural crest
Grant Data
Project Title
Define the core regulomes underlying Hirschsprung pathogenesis using single-cell transcriptomic analysis of human iPSC-derived enteric neural crest
Principal Investigator
Professor Ngan, Elly Sau Wai
(Principal Investigator (PI))
Duration
42
Start Date
2021-01-01
Amount
996285
Conference Title
Define the core regulomes underlying Hirschsprung pathogenesis using single-cell transcriptomic analysis of human iPSC-derived enteric neural crest
Keywords
Hirschsprung disease, human neural crest, iPSC-based disease model, single cell RNAseq
Discipline
Gastroenterology/HepatobiliaryGrowth and Development
Panel
Biology and Medicine (M)
HKU Project Code
17101320
Grant Type
General Research Fund (GRF)
Funding Year
2020
Status
On-going
Objectives
1 To define the key roles of HDAC1 underlying the human ENCC development. 2 To delineate elucidating the interplay between HDAC1, Hedgehog and Notch pathways in the neurogenic and gliogenic lineage differentiation of hiPSC-derived ENCCs 3 To decipher the key regulomes underlying the disease risk and severity through integrating the transcriptome and epigenome of human ENCCs