Development of new methods and strategies for extending Ser/Thr ligation utility in protein chemical synthesis
Grant Data
Project Title
Development of new methods and strategies for extending Ser/Thr ligation utility in protein chemical synthesis
Principal Investigator
Professor Li, Xuechen
(Principal Investigator (PI))
Duration
36
Start Date
2017-01-01
Amount
540824
Conference Title
Development of new methods and strategies for extending Ser/Thr ligation utility in protein chemical synthesis
Presentation Title
Keywords
peptide ligation, protein synthesis, solid phase peptide synthesis, synthetic protein chemistry
Discipline
Chemical Sciences
Panel
Physical Sciences (P)
HKU Project Code
17309616
Grant Type
General Research Fund (GRF)
Funding Year
2016
Status
Completed
Objectives
1 Develop a new method to prepare peptide salicylaldehyde esters via Boc-SPPS. 2 Develop on-resin Ser/Thr ligation and traceless on-resin ligation-mediated peptide cyclization. 3 Develop a homoserine ligation enabling the formation of Xaa-Asp at the ligation site. 4 Develop a method to improve Ser/Thr ligation using a solubilizing salicylaldehyde ester. 5 Develop new methods and strategies facilitating successive Ser/Thr ligation and apply this to the synthesis of the centra protein. For N to C Ser/Thr ligation, we will introduce a peptide salicylaldehyde