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Article: Cloud-point extraction and preconcentration of cyanobacterial toxins (microcystins) from natural waters using a cationic surfactant

TitleCloud-point extraction and preconcentration of cyanobacterial toxins (microcystins) from natural waters using a cationic surfactant
Authors
KeywordsSpecies Index: Anas Sp.
Bacteria (Microorganisms)
Cyanobacteria
Issue Date2002
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/est
Citation
Environmental Science And Technology, 2002, v. 36 n. 18, p. 3985-3990 How to Cite?
AbstractA new cloud-point extraction and preconcentration method using a cationic surfactant, Aliquat-336 (tricaprylyl-methylammonium chloride), has been developed for the determination of cyanobacterial toxins, microcystins, in natural waters. Sodium sulfate was used to induce phase separation at 25°C. The phase behavior of Aliquat-336 with respect to concentration of Na 2SO 4 was studied. The cloud-point system revealed a very high phase volume ratio compared to other established systems of nonionic, anionic, and cationic surfactants. At pH 6-7, it showed an outstanding selectivity in analyte extraction for anionic species. Only MC-LR and MC-YR, which are known to be predominantly anionic, were extracted (with averaged recoveries of 113.9 ± 9% and 87.1 ± 7%, respectively). MC-RR, which is likely to be amphoteric at the above pH range, was not detectable in the extract. Coupled to HPLC/UV separation and detection, the cloud-point extraction method (with 2.5 mM Aliquat-336 and 75 mM Na 2SO 4 at 25 °C) offered detection limits of 150 ± 7 and 470 ± 72 pg/mL for MC-LR and MC-YR, respectively, in 25 mL of deionized water. Repeatability of the method was 7.6% for MC-LR and 7.3% for MC-YR. The cloud-point extraction process can be completed within 10-15 min with no cleanup steps required. Applicability of the new method to the determination of microcystins in real samples was demonstrated using natural surface waters collected from a local river and a local duck pond spiked with realistic concentrations of microcystins. Effects of salinity and organic matter (TOC) content in the water sample on the extra ction efficiency were also studied.
Persistent Identifierhttp://hdl.handle.net/10722/92674
ISSN
2015 Impact Factor: 5.393
2015 SCImago Journal Rankings: 2.664
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMan, BKWen_HK
dc.contributor.authorLam, MHWen_HK
dc.contributor.authorLam, PKSen_HK
dc.contributor.authorWu, RSSen_HK
dc.contributor.authorShaw, Gen_HK
dc.date.accessioned2010-09-17T10:53:48Z-
dc.date.available2010-09-17T10:53:48Z-
dc.date.issued2002en_HK
dc.identifier.citationEnvironmental Science And Technology, 2002, v. 36 n. 18, p. 3985-3990en_HK
dc.identifier.issn0013-936Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/92674-
dc.description.abstractA new cloud-point extraction and preconcentration method using a cationic surfactant, Aliquat-336 (tricaprylyl-methylammonium chloride), has been developed for the determination of cyanobacterial toxins, microcystins, in natural waters. Sodium sulfate was used to induce phase separation at 25°C. The phase behavior of Aliquat-336 with respect to concentration of Na 2SO 4 was studied. The cloud-point system revealed a very high phase volume ratio compared to other established systems of nonionic, anionic, and cationic surfactants. At pH 6-7, it showed an outstanding selectivity in analyte extraction for anionic species. Only MC-LR and MC-YR, which are known to be predominantly anionic, were extracted (with averaged recoveries of 113.9 ± 9% and 87.1 ± 7%, respectively). MC-RR, which is likely to be amphoteric at the above pH range, was not detectable in the extract. Coupled to HPLC/UV separation and detection, the cloud-point extraction method (with 2.5 mM Aliquat-336 and 75 mM Na 2SO 4 at 25 °C) offered detection limits of 150 ± 7 and 470 ± 72 pg/mL for MC-LR and MC-YR, respectively, in 25 mL of deionized water. Repeatability of the method was 7.6% for MC-LR and 7.3% for MC-YR. The cloud-point extraction process can be completed within 10-15 min with no cleanup steps required. Applicability of the new method to the determination of microcystins in real samples was demonstrated using natural surface waters collected from a local river and a local duck pond spiked with realistic concentrations of microcystins. Effects of salinity and organic matter (TOC) content in the water sample on the extra ction efficiency were also studied.en_HK
dc.languageengen_HK
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/esten_HK
dc.relation.ispartofEnvironmental Science and Technologyen_HK
dc.subjectSpecies Index: Anas Sp.en_HK
dc.subjectBacteria (Microorganisms)en_HK
dc.subjectCyanobacteriaen_HK
dc.titleCloud-point extraction and preconcentration of cyanobacterial toxins (microcystins) from natural waters using a cationic surfactanten_HK
dc.typeArticleen_HK
dc.identifier.emailWu, RSS: rudolfwu@hku.hken_HK
dc.identifier.authorityWu, RSS=rp01398en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/es020620ven_HK
dc.identifier.pmid12269752-
dc.identifier.scopuseid_2-s2.0-0037105647en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037105647&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume36en_HK
dc.identifier.issue18en_HK
dc.identifier.spage3985en_HK
dc.identifier.epage3990en_HK
dc.identifier.isiWOS:000178016600014-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridMan, BKW=7003818463en_HK
dc.identifier.scopusauthoridLam, MHW=7202630175en_HK
dc.identifier.scopusauthoridLam, PKS=7202365776en_HK
dc.identifier.scopusauthoridWu, RSS=7402945079en_HK
dc.identifier.scopusauthoridShaw, G=7401773458en_HK

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