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Article: Semi-quantitative fluorescent PCR analysis identifies PRKAA1 on chromosome 5 as a potential candidate cancer gene of cervical cancer

TitleSemi-quantitative fluorescent PCR analysis identifies PRKAA1 on chromosome 5 as a potential candidate cancer gene of cervical cancer
Authors
KeywordsCandidate cancer genes
Cervical cancer
Comparative genomic hybridization
PRKAA1
Semi-quantitative fluorescent differential PCR
Issue Date2006
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygyno
Citation
Gynecologic Oncology, 2006, v. 103 n. 1, p. 219-225 How to Cite?
AbstractObjective.: Comparative genomic hybridization has frequently detected amplification of chromosome 5p in cervical cancer, but candidate cancer genes within the region are rarely known. Therefore, we pursued to identify potential candidate gene related to cervical cancer development. Methods.: A series of 128 cervical tumor samples were examined by semi-quantitative fluorescent differential PCR for copy number changes on three candidate genes (PRKAA1, CTNND2 and POLS) mapped to chromosome 5p and one gene (ERBIN) mapped to chromosome 5q12.3. The impact of gene copy number was later analyzed in relation to HPV infection, tumor stage or tumor radiosensitivity. Results.: DNA copy numbers of PRKAA1, CTNND2 and ERBIN were significantly different from normal controls (P < 0.05). DNA copy number changes did not correlate with HPV infection, tumor stages or tumor radiosensitivity. Using RT-PCR, PRKAA1 mRNA expression in seven tumor samples with known 5p amplification was amplified from 3- to 15-fold. Over-expression of PRKAA1 was further confirmed by immunohistochemical staining on 125 paraffin-embedded cervical cancer tissues. The expression level in cervical tumor was significantly higher than that in normal epithelium (P < 0.001). Conclusions.: PRKAA1 gene codes for the catalytic alpha 1 subunit of the AMP-activated protein kinase which is an important cellular metabolic stress regulator. It might assist tumor cells growth under stress. Thus, PRKAA1 may be one of the potential candidate genes for cervical carcinogenesis. © 2006 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/87081
ISSN
2015 Impact Factor: 4.198
2015 SCImago Journal Rankings: 2.284
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHuang, FYen_HK
dc.contributor.authorChiu, PMen_HK
dc.contributor.authorTam, KFen_HK
dc.contributor.authorKwok, YKYen_HK
dc.contributor.authorLau, ETen_HK
dc.contributor.authorTang, MHYen_HK
dc.contributor.authorNg, TYen_HK
dc.contributor.authorLiu, VWSen_HK
dc.contributor.authorCheung, ANYen_HK
dc.contributor.authorNgan, HYSen_HK
dc.date.accessioned2010-09-06T09:25:04Z-
dc.date.available2010-09-06T09:25:04Z-
dc.date.issued2006en_HK
dc.identifier.citationGynecologic Oncology, 2006, v. 103 n. 1, p. 219-225en_HK
dc.identifier.issn0090-8258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/87081-
dc.description.abstractObjective.: Comparative genomic hybridization has frequently detected amplification of chromosome 5p in cervical cancer, but candidate cancer genes within the region are rarely known. Therefore, we pursued to identify potential candidate gene related to cervical cancer development. Methods.: A series of 128 cervical tumor samples were examined by semi-quantitative fluorescent differential PCR for copy number changes on three candidate genes (PRKAA1, CTNND2 and POLS) mapped to chromosome 5p and one gene (ERBIN) mapped to chromosome 5q12.3. The impact of gene copy number was later analyzed in relation to HPV infection, tumor stage or tumor radiosensitivity. Results.: DNA copy numbers of PRKAA1, CTNND2 and ERBIN were significantly different from normal controls (P < 0.05). DNA copy number changes did not correlate with HPV infection, tumor stages or tumor radiosensitivity. Using RT-PCR, PRKAA1 mRNA expression in seven tumor samples with known 5p amplification was amplified from 3- to 15-fold. Over-expression of PRKAA1 was further confirmed by immunohistochemical staining on 125 paraffin-embedded cervical cancer tissues. The expression level in cervical tumor was significantly higher than that in normal epithelium (P < 0.001). Conclusions.: PRKAA1 gene codes for the catalytic alpha 1 subunit of the AMP-activated protein kinase which is an important cellular metabolic stress regulator. It might assist tumor cells growth under stress. Thus, PRKAA1 may be one of the potential candidate genes for cervical carcinogenesis. © 2006 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygynoen_HK
dc.relation.ispartofGynecologic Oncologyen_HK
dc.subjectCandidate cancer genesen_HK
dc.subjectCervical canceren_HK
dc.subjectComparative genomic hybridizationen_HK
dc.subjectPRKAA1en_HK
dc.subjectSemi-quantitative fluorescent differential PCRen_HK
dc.subject.meshAMP-Activated Protein Kinasesen_HK
dc.subject.meshAdaptor Proteins, Signal Transducing - geneticsen_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshCarcinoma, Squamous Cell - geneticsen_HK
dc.subject.meshChromosomal Proteins, Non-Histone - geneticsen_HK
dc.subject.meshChromosomes, Human, Pair 5 - geneticsen_HK
dc.subject.meshDNA, Neoplasm - geneticsen_HK
dc.subject.meshDNA, Viral - geneticsen_HK
dc.subject.meshDNA-Directed DNA Polymerase - geneticsen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Amplificationen_HK
dc.subject.meshGene Dosageen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshMultienzyme Complexes - geneticsen_HK
dc.subject.meshNuclear Proteins - geneticsen_HK
dc.subject.meshPapillomaviridae - geneticsen_HK
dc.subject.meshPapillomavirus Infections - complications - enzymology - geneticsen_HK
dc.subject.meshProtein-Serine-Threonine Kinases - geneticsen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction - methodsen_HK
dc.subject.meshUterine Cervical Neoplasms - enzymology - genetics - virologyen_HK
dc.titleSemi-quantitative fluorescent PCR analysis identifies PRKAA1 on chromosome 5 as a potential candidate cancer gene of cervical canceren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0090-8258&volume=103&issue=1&spage=219&epage=25&date=2006&atitle=Semi-quantitative+fluorescent+PCR+analysis+identifies+PRKAA1+on+chromosome+5+as+a+potential+candidate+cancer+gene+of+cervical+canceren_HK
dc.identifier.emailTang, MHY: mhytang@hkucc.hku.hken_HK
dc.identifier.emailLiu, VWS: vwsliu@hkusua.hku.hken_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hken_HK
dc.identifier.authorityTang, MHY=rp01701en_HK
dc.identifier.authorityLiu, VWS=rp00341en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ygyno.2006.02.028en_HK
dc.identifier.pmid16595147-
dc.identifier.scopuseid_2-s2.0-33748570853en_HK
dc.identifier.hkuros120435en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33748570853&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume103en_HK
dc.identifier.issue1en_HK
dc.identifier.spage219en_HK
dc.identifier.epage225en_HK
dc.identifier.isiWOS:000240887100041-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHuang, FY=8644138400en_HK
dc.identifier.scopusauthoridChiu, PM=7103182596en_HK
dc.identifier.scopusauthoridTam, KF=7201692816en_HK
dc.identifier.scopusauthoridKwok, YKY=8247106700en_HK
dc.identifier.scopusauthoridLau, ET=36006491400en_HK
dc.identifier.scopusauthoridTang, MHY=8943401300en_HK
dc.identifier.scopusauthoridNg, TY=7402229853en_HK
dc.identifier.scopusauthoridLiu, VWS=7006405113en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK

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