Article: Semi-quantitative fluorescent PCR analysis identifies PRKAA1 on chromosome 5 as a potential candidate cancer gene of cervical cancer

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TitleSemi-quantitative fluorescent PCR analysis identifies PRKAA1 on chromosome 5 as a potential candidate cancer gene of cervical cancer
AuthorsHuang, FY1
Chiu, PM1
Tam, KF1
Kwok, YKY1
Lau, ET2
Tang, MHY2
Ng, TY1
Liu, VWS1
Cheung, ANY1
Ngan, HYS1
KeywordsCandidate cancer genes
Cervical cancer
Comparative genomic hybridization
PRKAA1
Semi-quantitative fluorescent differential PCR
Issue Date2006
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygyno
CitationGynecologic Oncology, 2006, v. 103 n. 1, p. 219-225 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ygyno.2006.02.028
AbstractObjective.: Comparative genomic hybridization has frequently detected amplification of chromosome 5p in cervical cancer, but candidate cancer genes within the region are rarely known. Therefore, we pursued to identify potential candidate gene related to cervical cancer development. Methods.: A series of 128 cervical tumor samples were examined by semi-quantitative fluorescent differential PCR for copy number changes on three candidate genes (PRKAA1, CTNND2 and POLS) mapped to chromosome 5p and one gene (ERBIN) mapped to chromosome 5q12.3. The impact of gene copy number was later analyzed in relation to HPV infection, tumor stage or tumor radiosensitivity. Results.: DNA copy numbers of PRKAA1, CTNND2 and ERBIN were significantly different from normal controls (P < 0.05). DNA copy number changes did not correlate with HPV infection, tumor stages or tumor radiosensitivity. Using RT-PCR, PRKAA1 mRNA expression in seven tumor samples with known 5p amplification was amplified from 3- to 15-fold. Over-expression of PRKAA1 was further confirmed by immunohistochemical staining on 125 paraffin-embedded cervical cancer tissues. The expression level in cervical tumor was significantly higher than that in normal epithelium (P < 0.001). Conclusions.: PRKAA1 gene codes for the catalytic alpha 1 subunit of the AMP-activated protein kinase which is an important cellular metabolic stress regulator. It might assist tumor cells growth under stress. Thus, PRKAA1 may be one of the potential candidate genes for cervical carcinogenesis. © 2006 Elsevier Inc. All rights reserved.
ISSN0090-8258
2011 Impact Factor: 3.888
2011 SCImago Journal Rankings: 0.374
DOIhttp://dx.doi.org/10.1016/j.ygyno.2006.02.028
ISI Accession Number IDWOS:000240887100041
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorHuang, FY
dc.contributor.authorChiu, PM
dc.contributor.authorTam, KF
dc.contributor.authorKwok, YKY
dc.contributor.authorLau, ET
dc.contributor.authorTang, MHY
dc.contributor.authorNg, TY
dc.contributor.authorLiu, VWS
dc.contributor.authorCheung, ANY
dc.contributor.authorNgan, HYS
dc.date.accessioned2010-09-06T09:25:04Z
dc.date.available2010-09-06T09:25:04Z
dc.date.issued2006
dc.description.abstractObjective.: Comparative genomic hybridization has frequently detected amplification of chromosome 5p in cervical cancer, but candidate cancer genes within the region are rarely known. Therefore, we pursued to identify potential candidate gene related to cervical cancer development. Methods.: A series of 128 cervical tumor samples were examined by semi-quantitative fluorescent differential PCR for copy number changes on three candidate genes (PRKAA1, CTNND2 and POLS) mapped to chromosome 5p and one gene (ERBIN) mapped to chromosome 5q12.3. The impact of gene copy number was later analyzed in relation to HPV infection, tumor stage or tumor radiosensitivity. Results.: DNA copy numbers of PRKAA1, CTNND2 and ERBIN were significantly different from normal controls (P < 0.05). DNA copy number changes did not correlate with HPV infection, tumor stages or tumor radiosensitivity. Using RT-PCR, PRKAA1 mRNA expression in seven tumor samples with known 5p amplification was amplified from 3- to 15-fold. Over-expression of PRKAA1 was further confirmed by immunohistochemical staining on 125 paraffin-embedded cervical cancer tissues. The expression level in cervical tumor was significantly higher than that in normal epithelium (P < 0.001). Conclusions.: PRKAA1 gene codes for the catalytic alpha 1 subunit of the AMP-activated protein kinase which is an important cellular metabolic stress regulator. It might assist tumor cells growth under stress. Thus, PRKAA1 may be one of the potential candidate genes for cervical carcinogenesis. © 2006 Elsevier Inc. All rights reserved.
dc.description.naturelink_to_subscribed_fulltext
dc.identifier.citationGynecologic Oncology, 2006, v. 103 n. 1, p. 219-225 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ygyno.2006.02.028
dc.identifier.doihttp://dx.doi.org/10.1016/j.ygyno.2006.02.028
dc.identifier.epage225
dc.identifier.hkuros120435
dc.identifier.isiWOS:000240887100041
dc.identifier.issn0090-8258
2011 Impact Factor: 3.888
2011 SCImago Journal Rankings: 0.374
dc.identifier.issue1
dc.identifier.openurl
dc.identifier.pmid16595147
dc.identifier.scopuseid_2-s2.0-33748570853
dc.identifier.spage219
dc.identifier.urihttp://hdl.handle.net/10722/87081
dc.identifier.volume103
dc.languageeng
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygyno
dc.publisher.placeUnited States
dc.relation.ispartofGynecologic Oncology
dc.relation.referencesReferences in Scopus
dc.subject.meshAMP-Activated Protein Kinases
dc.subject.meshAdaptor Proteins, Signal Transducing - genetics
dc.subject.meshAdult
dc.subject.meshAged
dc.subject.meshCarcinoma, Squamous Cell - genetics
dc.subject.meshChromosomal Proteins, Non-Histone - genetics
dc.subject.meshChromosomes, Human, Pair 5 - genetics
dc.subject.meshDNA, Neoplasm - genetics
dc.subject.meshDNA, Viral - genetics
dc.subject.meshDNA-Directed DNA Polymerase - genetics
dc.subject.meshFemale
dc.subject.meshGene Amplification
dc.subject.meshGene Dosage
dc.subject.meshHumans
dc.subject.meshMiddle Aged
dc.subject.meshMultienzyme Complexes - genetics
dc.subject.meshNuclear Proteins - genetics
dc.subject.meshPapillomaviridae - genetics
dc.subject.meshPapillomavirus Infections - complications - enzymology - genetics
dc.subject.meshProtein-Serine-Threonine Kinases - genetics
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction - methods
dc.subject.meshUterine Cervical Neoplasms - enzymology - genetics - virology
dc.subjectCandidate cancer genes
dc.subjectCervical cancer
dc.subjectComparative genomic hybridization
dc.subjectPRKAA1
dc.subjectSemi-quantitative fluorescent differential PCR
dc.titleSemi-quantitative fluorescent PCR analysis identifies PRKAA1 on chromosome 5 as a potential candidate cancer gene of cervical cancer
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Tsan Yuk Hospital