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Article: Semi-quantitative fluorescent PCR analysis identifies PRKAA1 on chromosome 5 as a potential candidate cancer gene of cervical cancer
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TitleSemi-quantitative fluorescent PCR analysis identifies PRKAA1 on chromosome 5 as a potential candidate cancer gene of cervical cancer
 
AuthorsHuang, FY1
Chiu, PM1
Tam, KF1
Kwok, YKY1
Lau, ET2
Tang, MHY2
Ng, TY1
Liu, VWS1
Cheung, ANY1
Ngan, HYS1
 
KeywordsCandidate cancer genes
Cervical cancer
Comparative genomic hybridization
PRKAA1
Semi-quantitative fluorescent differential PCR
 
Issue Date2006
 
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygyno
 
CitationGynecologic Oncology, 2006, v. 103 n. 1, p. 219-225 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ygyno.2006.02.028
 
AbstractObjective.: Comparative genomic hybridization has frequently detected amplification of chromosome 5p in cervical cancer, but candidate cancer genes within the region are rarely known. Therefore, we pursued to identify potential candidate gene related to cervical cancer development. Methods.: A series of 128 cervical tumor samples were examined by semi-quantitative fluorescent differential PCR for copy number changes on three candidate genes (PRKAA1, CTNND2 and POLS) mapped to chromosome 5p and one gene (ERBIN) mapped to chromosome 5q12.3. The impact of gene copy number was later analyzed in relation to HPV infection, tumor stage or tumor radiosensitivity. Results.: DNA copy numbers of PRKAA1, CTNND2 and ERBIN were significantly different from normal controls (P < 0.05). DNA copy number changes did not correlate with HPV infection, tumor stages or tumor radiosensitivity. Using RT-PCR, PRKAA1 mRNA expression in seven tumor samples with known 5p amplification was amplified from 3- to 15-fold. Over-expression of PRKAA1 was further confirmed by immunohistochemical staining on 125 paraffin-embedded cervical cancer tissues. The expression level in cervical tumor was significantly higher than that in normal epithelium (P < 0.001). Conclusions.: PRKAA1 gene codes for the catalytic alpha 1 subunit of the AMP-activated protein kinase which is an important cellular metabolic stress regulator. It might assist tumor cells growth under stress. Thus, PRKAA1 may be one of the potential candidate genes for cervical carcinogenesis. © 2006 Elsevier Inc. All rights reserved.
 
ISSN0090-8258
2012 Impact Factor: 3.929
2012 SCImago Journal Rankings: 1.685
 
DOIhttp://dx.doi.org/10.1016/j.ygyno.2006.02.028
 
ISI Accession Number IDWOS:000240887100041
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorHuang, FY
 
dc.contributor.authorChiu, PM
 
dc.contributor.authorTam, KF
 
dc.contributor.authorKwok, YKY
 
dc.contributor.authorLau, ET
 
dc.contributor.authorTang, MHY
 
dc.contributor.authorNg, TY
 
dc.contributor.authorLiu, VWS
 
dc.contributor.authorCheung, ANY
 
dc.contributor.authorNgan, HYS
 
dc.date.accessioned2010-09-06T09:25:04Z
 
dc.date.available2010-09-06T09:25:04Z
 
dc.date.issued2006
 
dc.description.abstractObjective.: Comparative genomic hybridization has frequently detected amplification of chromosome 5p in cervical cancer, but candidate cancer genes within the region are rarely known. Therefore, we pursued to identify potential candidate gene related to cervical cancer development. Methods.: A series of 128 cervical tumor samples were examined by semi-quantitative fluorescent differential PCR for copy number changes on three candidate genes (PRKAA1, CTNND2 and POLS) mapped to chromosome 5p and one gene (ERBIN) mapped to chromosome 5q12.3. The impact of gene copy number was later analyzed in relation to HPV infection, tumor stage or tumor radiosensitivity. Results.: DNA copy numbers of PRKAA1, CTNND2 and ERBIN were significantly different from normal controls (P < 0.05). DNA copy number changes did not correlate with HPV infection, tumor stages or tumor radiosensitivity. Using RT-PCR, PRKAA1 mRNA expression in seven tumor samples with known 5p amplification was amplified from 3- to 15-fold. Over-expression of PRKAA1 was further confirmed by immunohistochemical staining on 125 paraffin-embedded cervical cancer tissues. The expression level in cervical tumor was significantly higher than that in normal epithelium (P < 0.001). Conclusions.: PRKAA1 gene codes for the catalytic alpha 1 subunit of the AMP-activated protein kinase which is an important cellular metabolic stress regulator. It might assist tumor cells growth under stress. Thus, PRKAA1 may be one of the potential candidate genes for cervical carcinogenesis. © 2006 Elsevier Inc. All rights reserved.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationGynecologic Oncology, 2006, v. 103 n. 1, p. 219-225 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ygyno.2006.02.028
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.ygyno.2006.02.028
 
dc.identifier.epage225
 
dc.identifier.hkuros120435
 
dc.identifier.isiWOS:000240887100041
 
dc.identifier.issn0090-8258
2012 Impact Factor: 3.929
2012 SCImago Journal Rankings: 1.685
 
dc.identifier.issue1
 
dc.identifier.openurl
 
dc.identifier.pmid16595147
 
dc.identifier.scopuseid_2-s2.0-33748570853
 
dc.identifier.spage219
 
dc.identifier.urihttp://hdl.handle.net/10722/87081
 
dc.identifier.volume103
 
dc.languageeng
 
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ygyno
 
dc.publisher.placeUnited States
 
dc.relation.ispartofGynecologic Oncology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAMP-Activated Protein Kinases
 
dc.subject.meshAdaptor Proteins, Signal Transducing - genetics
 
dc.subject.meshAdult
 
dc.subject.meshAged
 
dc.subject.meshCarcinoma, Squamous Cell - genetics
 
dc.subject.meshChromosomal Proteins, Non-Histone - genetics
 
dc.subject.meshChromosomes, Human, Pair 5 - genetics
 
dc.subject.meshDNA, Neoplasm - genetics
 
dc.subject.meshDNA, Viral - genetics
 
dc.subject.meshDNA-Directed DNA Polymerase - genetics
 
dc.subject.meshFemale
 
dc.subject.meshGene Amplification
 
dc.subject.meshGene Dosage
 
dc.subject.meshHumans
 
dc.subject.meshMiddle Aged
 
dc.subject.meshMultienzyme Complexes - genetics
 
dc.subject.meshNuclear Proteins - genetics
 
dc.subject.meshPapillomaviridae - genetics
 
dc.subject.meshPapillomavirus Infections - complications - enzymology - genetics
 
dc.subject.meshProtein-Serine-Threonine Kinases - genetics
 
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction - methods
 
dc.subject.meshUterine Cervical Neoplasms - enzymology - genetics - virology
 
dc.subjectCandidate cancer genes
 
dc.subjectCervical cancer
 
dc.subjectComparative genomic hybridization
 
dc.subjectPRKAA1
 
dc.subjectSemi-quantitative fluorescent differential PCR
 
dc.titleSemi-quantitative fluorescent PCR analysis identifies PRKAA1 on chromosome 5 as a potential candidate cancer gene of cervical cancer
 
dc.typeArticle
 
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<contributor.author>Chiu, PM</contributor.author>
<contributor.author>Tam, KF</contributor.author>
<contributor.author>Kwok, YKY</contributor.author>
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<contributor.author>Tang, MHY</contributor.author>
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<description.abstract>Objective.: Comparative genomic hybridization has frequently detected amplification of chromosome 5p in cervical cancer, but candidate cancer genes within the region are rarely known. Therefore, we pursued to identify potential candidate gene related to cervical cancer development. Methods.: A series of 128 cervical tumor samples were examined by semi-quantitative fluorescent differential PCR for copy number changes on three candidate genes (PRKAA1, CTNND2 and POLS) mapped to chromosome 5p and one gene (ERBIN) mapped to chromosome 5q12.3. The impact of gene copy number was later analyzed in relation to HPV infection, tumor stage or tumor radiosensitivity. Results.: DNA copy numbers of PRKAA1, CTNND2 and ERBIN were significantly different from normal controls (P&#160;&lt;&#160;0.05). DNA copy number changes did not correlate with HPV infection, tumor stages or tumor radiosensitivity. Using RT-PCR, PRKAA1 mRNA expression in seven tumor samples with known 5p amplification was amplified from 3- to 15-fold. Over-expression of PRKAA1 was further confirmed by immunohistochemical staining on 125 paraffin-embedded cervical cancer tissues. The expression level in cervical tumor was significantly higher than that in normal epithelium (P&#160;&lt;&#160;0.001). Conclusions.: PRKAA1 gene codes for the catalytic alpha 1 subunit of the AMP-activated protein kinase which is an important cellular metabolic stress regulator. It might assist tumor cells growth under stress. Thus, PRKAA1 may be one of the potential candidate genes for cervical carcinogenesis. &#169; 2006 Elsevier Inc. All rights reserved.</description.abstract>
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<subject.mesh>Protein-Serine-Threonine Kinases - genetics</subject.mesh>
<subject.mesh>Reverse Transcriptase Polymerase Chain Reaction - methods</subject.mesh>
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Author Affiliations
  1. The University of Hong Kong
  2. Tsan Yuk Hospital