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Article: Characterization of Guangzhou Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Field Isolates

TitleCharacterization of Guangzhou Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Field Isolates
Authors
Issue Date2003
PublisherAsian Network for Scientific Information (A N S I N E T). The Journal's web site is located at http://www.medwellonline.net/java/fp.html
Citation
Journal of Animal and Veterinary Advances, 2003, v. 2 n. 8, p. 452-456 How to Cite?
AbstractA direct method was used to detect the presence of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) in tissue samples of infected pigs collected from different farms in Guangzhou, based on reverse transcription of the viral RNA coupled to DNA amplification by polymerase chain reaction (RT-PCR). Several sets of primers were designed based on both the North American (VR-2332) and the European (LV) genome sequences within ORF1b encoding the polymerase protein in order to perform a rapid multiplex PCR assay. DNA products with unique size characteristics of each genotype were obtained. From three (named D2, D3 & AV) Guangzhou field isolates, amplified fragment of 108 bp was cloned and sequenced. Alignment with North American strain (VR-2332) sequences revealed a 95.5% homology. Among all, AV Guangzhou strain tissue homogenates were inoculated into MARC-145 cells and after the sixth passage, cytopathic effects (CPE) of infected cell were observed. Analysis of purified virions of AV Guangzhou strain by SDS-PAGE and western immunoblotting using porcine hyperimmune sera revealed that four major viral proteins: 15, 19, 26 and 45 kDa proteins.
Persistent Identifierhttp://hdl.handle.net/10722/84951
ISSN
2012 Impact Factor: 0.365
2015 SCImago Journal Rankings: 0.160

 

DC FieldValueLanguage
dc.contributor.authorWong, EYLen_HK
dc.contributor.authorWu, Hen_HK
dc.contributor.authorLeung, FCCen_HK
dc.date.accessioned2010-09-06T08:59:01Z-
dc.date.available2010-09-06T08:59:01Z-
dc.date.issued2003en_HK
dc.identifier.citationJournal of Animal and Veterinary Advances, 2003, v. 2 n. 8, p. 452-456en_HK
dc.identifier.issn1680-5593en_HK
dc.identifier.urihttp://hdl.handle.net/10722/84951-
dc.description.abstractA direct method was used to detect the presence of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) in tissue samples of infected pigs collected from different farms in Guangzhou, based on reverse transcription of the viral RNA coupled to DNA amplification by polymerase chain reaction (RT-PCR). Several sets of primers were designed based on both the North American (VR-2332) and the European (LV) genome sequences within ORF1b encoding the polymerase protein in order to perform a rapid multiplex PCR assay. DNA products with unique size characteristics of each genotype were obtained. From three (named D2, D3 & AV) Guangzhou field isolates, amplified fragment of 108 bp was cloned and sequenced. Alignment with North American strain (VR-2332) sequences revealed a 95.5% homology. Among all, AV Guangzhou strain tissue homogenates were inoculated into MARC-145 cells and after the sixth passage, cytopathic effects (CPE) of infected cell were observed. Analysis of purified virions of AV Guangzhou strain by SDS-PAGE and western immunoblotting using porcine hyperimmune sera revealed that four major viral proteins: 15, 19, 26 and 45 kDa proteins.-
dc.languageengen_HK
dc.publisherAsian Network for Scientific Information (A N S I N E T). The Journal's web site is located at http://www.medwellonline.net/java/fp.htmlen_HK
dc.relation.ispartofJournal of Animal and Veterinary Advancesen_HK
dc.titleCharacterization of Guangzhou Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Field Isolatesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1680-5593&volume=2&spage=452&epage=456&date=2003&atitle=Characterization+of+Guangzhou+Porcine+Reproductive+and+Respiratory+Syndrome+Virus+(PRRSV)+Field+Isolatesen_HK
dc.identifier.emailLeung, FCC: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityLeung, FCC=rp00731en_HK
dc.identifier.hkuros85847en_HK

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