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Article: Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice
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TitleIntranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice
 
AuthorsQu, D2
Zheng, B1
Yao, X2
Guan, Y1
Yuan, ZH2
Zhong, NS4
Lu, LW1
Xie, JP3
Wen, YM2
 
KeywordsInactivated vaccine
Intranasal immunization
SARS-CoV
 
Issue Date2005
 
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/vaccine
 
CitationVaccine, 2005, v. 23 n. 7, p. 924-931 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.vaccine.2004.07.031
 
AbstractSARS-CoV (severe acute respiratory syndrome-associated coronavirus) strain GZ50 was partially purified and inactivated with 1:2000 formaldehyde. In cell culture the inactivated virus blocked the replication of live virus by decreasing the TCID 5.0 of the live virus 10 3.6 to 10 4.6 times. Inactivated GZ50 was used to immunize mice intranasally either alone, or after precipitation with polyethylene glycol (PEG), or with CpG, or CTB as an adjuvant. The titer of serum neutralizing antibodies was up to 1:640. In mice immunized with adjuvants or PEG precipitated GZ50, specific IgA was detected in tracheal-lung wash fluid by immunofluorescence. Though serum antibodies were detected, no anti-SARS-IgA could be detected in mice immunized only with inactivated GZ50. The roles of adjuvants in intranasal immunization with inactivated. SARS-CoV is discussed. © 2004 Elsevier Ltd. All rights reserved.
 
ISSN0264-410X
2012 Impact Factor: 3.492
2012 SCImago Journal Rankings: 1.360
 
DOIhttp://dx.doi.org/10.1016/j.vaccine.2004.07.031
 
ISI Accession Number IDWOS:000226326400011
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorQu, D
 
dc.contributor.authorZheng, B
 
dc.contributor.authorYao, X
 
dc.contributor.authorGuan, Y
 
dc.contributor.authorYuan, ZH
 
dc.contributor.authorZhong, NS
 
dc.contributor.authorLu, LW
 
dc.contributor.authorXie, JP
 
dc.contributor.authorWen, YM
 
dc.date.accessioned2010-09-06T07:50:07Z
 
dc.date.available2010-09-06T07:50:07Z
 
dc.date.issued2005
 
dc.description.abstractSARS-CoV (severe acute respiratory syndrome-associated coronavirus) strain GZ50 was partially purified and inactivated with 1:2000 formaldehyde. In cell culture the inactivated virus blocked the replication of live virus by decreasing the TCID 5.0 of the live virus 10 3.6 to 10 4.6 times. Inactivated GZ50 was used to immunize mice intranasally either alone, or after precipitation with polyethylene glycol (PEG), or with CpG, or CTB as an adjuvant. The titer of serum neutralizing antibodies was up to 1:640. In mice immunized with adjuvants or PEG precipitated GZ50, specific IgA was detected in tracheal-lung wash fluid by immunofluorescence. Though serum antibodies were detected, no anti-SARS-IgA could be detected in mice immunized only with inactivated GZ50. The roles of adjuvants in intranasal immunization with inactivated. SARS-CoV is discussed. © 2004 Elsevier Ltd. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationVaccine, 2005, v. 23 n. 7, p. 924-931 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.vaccine.2004.07.031
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.vaccine.2004.07.031
 
dc.identifier.epage931
 
dc.identifier.hkuros99117
 
dc.identifier.isiWOS:000226326400011
 
dc.identifier.issn0264-410X
2012 Impact Factor: 3.492
2012 SCImago Journal Rankings: 1.360
 
dc.identifier.issue7
 
dc.identifier.openurl
 
dc.identifier.pmid15603894
 
dc.identifier.scopuseid_2-s2.0-10444272332
 
dc.identifier.spage924
 
dc.identifier.urihttp://hdl.handle.net/10722/79061
 
dc.identifier.volume23
 
dc.languageeng
 
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/vaccine
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofVaccine
 
dc.relation.referencesReferences in Scopus
 
dc.rightsVaccine. Copyright © Elsevier Ltd.
 
dc.subject.meshAdministration, Intranasal
 
dc.subject.meshAnimals
 
dc.subject.meshAntibodies, Viral - biosynthesis - blood
 
dc.subject.meshCercopithecus aethiops
 
dc.subject.meshFemale
 
dc.subject.meshHumans
 
dc.subject.meshLung - immunology - metabolism
 
dc.subject.meshMale
 
dc.subject.meshMice
 
dc.subject.meshMice, Inbred BALB C
 
dc.subject.meshSARS Virus - immunology
 
dc.subject.meshVaccines, Inactivated - administration & dosage - immunology
 
dc.subject.meshVero Cells
 
dc.subject.meshViral Vaccines - administration & dosage - immunology
 
dc.subjectInactivated vaccine
 
dc.subjectIntranasal immunization
 
dc.subjectSARS-CoV
 
dc.titleIntranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice
 
dc.typeArticle
 
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<contributor.author>Yuan, ZH</contributor.author>
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<contributor.author>Lu, LW</contributor.author>
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Author Affiliations
  1. The University of Hong Kong
  2. Fudan University
  3. Guangzhou Children's Hospital
  4. Guangzhou Medical College