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Article: Silver nanoparticles inhibit hepatitis B virus replication

TitleSilver nanoparticles inhibit hepatitis B virus replication
Authors
Issue Date2008
PublisherInternational Medical Press. The Journal's web site is located at http://www.intmedpress.com/Journals/AVT/journals_avt_home.cfm
Citation
Antiviral Therapy, 2008, v. 13 n. 2, p. 252-262 How to Cite?
AbstractBackground: Silver nanoparticles have been shown to exhibit promising cytoprotective activities towards HIV-infected T-cells; however, the effects of these nanoparticles towards other kinds of viruses remain largely unexplored. The aim of the present study was to investigate the effects of silver nanoparticles on hepatitis B virus (HBV). Methods: Monodisperse silver nanoparticles with mean particle diameters of ∼10 nm (Ag10Ns) and ∼50 nm (Ag50Ns) were prepared from AgNO 3 in HEPES buffer. The in vitro anti-HBV activities of these particles were determined using the HepAD38 cell line as infection model. Results: Ag10Ns and Ag50Ns were able to reduce the extracellular HBV DNA formation of HepAD38 cells by >50% compared with the vehicle control (that is, HepAD38 cells in the absence of silver nanoparticles). Silver nanoparticles had little effect on the amount of HBV covalently closed circular DNA (cccDNA), but could inhibit the formation of intracellular HBV RNA. Gel mobility shift assays indicated that Ag10Ns bound HBV double-stranded DNA at a DNA:silver molar ratio of 1:50; an absorption titration assay showed that the nanoparticles have good binding affinity for HBV DNA with a binding constant (K b) of (8.8 ± 1.0)×10 5 dm 3mol -1. As both the viral and Ag10Ns systems are in the nanometer size range, we found that Ag10Ns could directly interact with the HBV viral particles as revealed by transmission electronic microscopy. Conclusions: Silver nanoparticles could inhibit the in vitro production of HBV RNA and extracellular virions. We hypothesize that the direct interaction between these nanoparticles and HBV double-stranded DNA or viral particles is responsible for their antiviral mechanism. © 2008 International Medical Press.
Persistent Identifierhttp://hdl.handle.net/10722/70405
ISSN
2015 Impact Factor: 2.916
2015 SCImago Journal Rankings: 1.361
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLu, Len_HK
dc.contributor.authorSun, RWYen_HK
dc.contributor.authorChen, Ren_HK
dc.contributor.authorHui, CKen_HK
dc.contributor.authorHo, CMen_HK
dc.contributor.authorLuk, JMen_HK
dc.contributor.authorLau, GKKen_HK
dc.contributor.authorChe, CMen_HK
dc.date.accessioned2010-09-06T06:22:34Z-
dc.date.available2010-09-06T06:22:34Z-
dc.date.issued2008en_HK
dc.identifier.citationAntiviral Therapy, 2008, v. 13 n. 2, p. 252-262en_HK
dc.identifier.issn1359-6535en_HK
dc.identifier.urihttp://hdl.handle.net/10722/70405-
dc.description.abstractBackground: Silver nanoparticles have been shown to exhibit promising cytoprotective activities towards HIV-infected T-cells; however, the effects of these nanoparticles towards other kinds of viruses remain largely unexplored. The aim of the present study was to investigate the effects of silver nanoparticles on hepatitis B virus (HBV). Methods: Monodisperse silver nanoparticles with mean particle diameters of ∼10 nm (Ag10Ns) and ∼50 nm (Ag50Ns) were prepared from AgNO 3 in HEPES buffer. The in vitro anti-HBV activities of these particles were determined using the HepAD38 cell line as infection model. Results: Ag10Ns and Ag50Ns were able to reduce the extracellular HBV DNA formation of HepAD38 cells by >50% compared with the vehicle control (that is, HepAD38 cells in the absence of silver nanoparticles). Silver nanoparticles had little effect on the amount of HBV covalently closed circular DNA (cccDNA), but could inhibit the formation of intracellular HBV RNA. Gel mobility shift assays indicated that Ag10Ns bound HBV double-stranded DNA at a DNA:silver molar ratio of 1:50; an absorption titration assay showed that the nanoparticles have good binding affinity for HBV DNA with a binding constant (K b) of (8.8 ± 1.0)×10 5 dm 3mol -1. As both the viral and Ag10Ns systems are in the nanometer size range, we found that Ag10Ns could directly interact with the HBV viral particles as revealed by transmission electronic microscopy. Conclusions: Silver nanoparticles could inhibit the in vitro production of HBV RNA and extracellular virions. We hypothesize that the direct interaction between these nanoparticles and HBV double-stranded DNA or viral particles is responsible for their antiviral mechanism. © 2008 International Medical Press.en_HK
dc.languageengen_HK
dc.publisherInternational Medical Press. The Journal's web site is located at http://www.intmedpress.com/Journals/AVT/journals_avt_home.cfmen_HK
dc.relation.ispartofAntiviral Therapyen_HK
dc.titleSilver nanoparticles inhibit hepatitis B virus replicationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1359-6535&volume=13&issue=2&spage=253&epage=262&date=2008&atitle=Silver+nanoparticles+inhibit+hepatitis+B+virus+replicationen_HK
dc.identifier.emailSun, RWY: rwysun@hku.hken_HK
dc.identifier.emailHo, CM: rickyho@hkucc.hku.hken_HK
dc.identifier.emailLuk, JM: jmluk@hkucc.hku.hken_HK
dc.identifier.emailChe, CM: cmche@hku.hken_HK
dc.identifier.authoritySun, RWY=rp00781en_HK
dc.identifier.authorityHo, CM=rp00705en_HK
dc.identifier.authorityLuk, JM=rp00349en_HK
dc.identifier.authorityChe, CM=rp00670en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid18505176-
dc.identifier.scopuseid_2-s2.0-42149148881en_HK
dc.identifier.hkuros146017en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-42149148881&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume13en_HK
dc.identifier.issue2en_HK
dc.identifier.spage252en_HK
dc.identifier.epage262en_HK
dc.identifier.isiWOS:000254969200010-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLu, L=55257185900en_HK
dc.identifier.scopusauthoridSun, RWY=26325835800en_HK
dc.identifier.scopusauthoridChen, R=9275915200en_HK
dc.identifier.scopusauthoridHui, CK=7202876933en_HK
dc.identifier.scopusauthoridHo, CM=12807243800en_HK
dc.identifier.scopusauthoridLuk, JM=7006777791en_HK
dc.identifier.scopusauthoridLau, GKK=7102301257en_HK
dc.identifier.scopusauthoridChe, CM=7102442791en_HK

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