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Article: Expression of cysteine proteinase during developmental events associated with programmed cell death in brinjal

TitleExpression of cysteine proteinase during developmental events associated with programmed cell death in brinjal
Authors
Issue Date1999
PublisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0960-7412
Citation
Plant Journal, 1999, v. 17 n. 3, p. 321-327 How to Cite?
AbstractHere we show that the expression of a cysteine proteinase coincides with several developmental events associated with programmed cell death (PCD) in Solanum melongena (brinjal), i.e. during leaf senescence, fruit senescence, xylogenesis, nucellar cell degeneration and anther senescence. We have isolated a cDNA encoding brinjal cysteine proteinase (SmCP) that shares high (90-92%) amino acid identity to cysteine proteinases of tobacco (CYP-8) and tomato (LCYP-2) that have not been previously reported to be senescence-associated. In contrast, SmCP shows lower (39-41%) amino acid identity to other senescence-related cysteine proteinases and, unlike most of them, it is not preferentially expressed in certain organs or cell types. Northern analysis of leaves, fruits and flowers at different stages of development showed that SmCP expression increased significantly at senescence in leaf and fruit, but was highly expressed throughout flower development. In situ hybridization studies on flower sections using an antisense RNA probe localized the SmCP mRNA to the xylem, the epidermis and the endothecium of the anther and the nucellar cells, suggesting its involvement in PCD during xylogenesis, anther senescence and ovule development, respectively. Its expression during nucellar cell degeneration suggests that protein reserves of the nucellus are released to the developing embryo. Polarity in its pattern of expression in the nucellus of the developing seed (40DAP) further implies a directional flow of these nutrients.
Persistent Identifierhttp://hdl.handle.net/10722/68424
ISSN
2015 Impact Factor: 5.468
2015 SCImago Journal Rankings: 3.457
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXu, FXen_HK
dc.contributor.authorChye, MLen_HK
dc.date.accessioned2010-09-06T06:04:30Z-
dc.date.available2010-09-06T06:04:30Z-
dc.date.issued1999en_HK
dc.identifier.citationPlant Journal, 1999, v. 17 n. 3, p. 321-327en_HK
dc.identifier.issn0960-7412en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68424-
dc.description.abstractHere we show that the expression of a cysteine proteinase coincides with several developmental events associated with programmed cell death (PCD) in Solanum melongena (brinjal), i.e. during leaf senescence, fruit senescence, xylogenesis, nucellar cell degeneration and anther senescence. We have isolated a cDNA encoding brinjal cysteine proteinase (SmCP) that shares high (90-92%) amino acid identity to cysteine proteinases of tobacco (CYP-8) and tomato (LCYP-2) that have not been previously reported to be senescence-associated. In contrast, SmCP shows lower (39-41%) amino acid identity to other senescence-related cysteine proteinases and, unlike most of them, it is not preferentially expressed in certain organs or cell types. Northern analysis of leaves, fruits and flowers at different stages of development showed that SmCP expression increased significantly at senescence in leaf and fruit, but was highly expressed throughout flower development. In situ hybridization studies on flower sections using an antisense RNA probe localized the SmCP mRNA to the xylem, the epidermis and the endothecium of the anther and the nucellar cells, suggesting its involvement in PCD during xylogenesis, anther senescence and ovule development, respectively. Its expression during nucellar cell degeneration suggests that protein reserves of the nucellus are released to the developing embryo. Polarity in its pattern of expression in the nucellus of the developing seed (40DAP) further implies a directional flow of these nutrients.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0960-7412en_HK
dc.relation.ispartofPlant Journalen_HK
dc.rightsThe Plant Journal. Copyright © Blackwell Publishing Ltd.en_HK
dc.titleExpression of cysteine proteinase during developmental events associated with programmed cell death in brinjalen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0960-7412&volume=17&spage=321&epage=327&date=1999&atitle=Expression+of+cysteine+proteinase+during+developmental+events+associated+with+programmed+cell+death+in+brinjalen_HK
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_HK
dc.identifier.authorityChye, ML=rp00687en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1365-313X.1999.00370.xen_HK
dc.identifier.pmid10097390-
dc.identifier.scopuseid_2-s2.0-0033083491en_HK
dc.identifier.hkuros46517en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033083491&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume17en_HK
dc.identifier.issue3en_HK
dc.identifier.spage321en_HK
dc.identifier.epage327en_HK
dc.identifier.isiWOS:000078780600010-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridXu, FX=7401695108en_HK
dc.identifier.scopusauthoridChye, ML=7003905460en_HK

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