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Article: Prokineticin-1 modulates proliferation and differentiation of enteric neural crest cells

TitleProkineticin-1 modulates proliferation and differentiation of enteric neural crest cells
Authors
KeywordsDifferentiation
Neural crest cell
Prokineticin-1
Proliferation
Issue Date2007
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbamcr
Citation
Biochimica Et Biophysica Acta - Molecular Cell Research, 2007, v. 1773 n. 4, p. 536-545 How to Cite?
AbstractProkineticins (Prok-1 and Prok-2) belong to a newly identified AVIT protein family. They are involved in variety of activities in various tissues, including smooth muscle contraction of the gastrointestinal tract and promoting proliferation of endothelial cells derived from adrenal gland. Importantly, they also act as the survival factors to modulate growth and survival of neurons and hematopoietic stem cells. In this study we demonstrated that Prok-1 (but not Prok-2) protein is expressed in the mucosa and mesenchyme of the mouse embryonic gut during enteric nervous system development. Its receptor, PK-R1 is expressed in the enteric neural crest cells (NCCs). To elucidate the physiological role(s) of Prok-1 in NCCs, we isolated the NCCs from the mouse embryonic gut (E11.5) and cultured them in the form of neurospheres. In an in vitro NCC culture, Prok-1 was able to activate both Akt and MAPK pathways and induce the proliferation and differentiation (but not migration) of NCCs via PK-R1. Knock-down of PK-R1 using siRNA resulted in a complete abolishment of Prok-1 induced proliferation. Taken together, it is the first report demonstrating that Prok-1 acts as a gut mucosa/mesenchyme-derived factor and maintains proliferation and differentiation of enteric NCCs. © 2007 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68290
ISSN
2015 Impact Factor: 5.128
2015 SCImago Journal Rankings: 3.043
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNgan, ESWen_HK
dc.contributor.authorLee, KYen_HK
dc.contributor.authorSit, FYLen_HK
dc.contributor.authorPoon, HCen_HK
dc.contributor.authorChan, JKYen_HK
dc.contributor.authorSham, MHen_HK
dc.contributor.authorLui, VCHen_HK
dc.contributor.authorTam, PKHen_HK
dc.date.accessioned2010-09-06T06:03:10Z-
dc.date.available2010-09-06T06:03:10Z-
dc.date.issued2007en_HK
dc.identifier.citationBiochimica Et Biophysica Acta - Molecular Cell Research, 2007, v. 1773 n. 4, p. 536-545en_HK
dc.identifier.issn0167-4889en_HK
dc.identifier.urihttp://hdl.handle.net/10722/68290-
dc.description.abstractProkineticins (Prok-1 and Prok-2) belong to a newly identified AVIT protein family. They are involved in variety of activities in various tissues, including smooth muscle contraction of the gastrointestinal tract and promoting proliferation of endothelial cells derived from adrenal gland. Importantly, they also act as the survival factors to modulate growth and survival of neurons and hematopoietic stem cells. In this study we demonstrated that Prok-1 (but not Prok-2) protein is expressed in the mucosa and mesenchyme of the mouse embryonic gut during enteric nervous system development. Its receptor, PK-R1 is expressed in the enteric neural crest cells (NCCs). To elucidate the physiological role(s) of Prok-1 in NCCs, we isolated the NCCs from the mouse embryonic gut (E11.5) and cultured them in the form of neurospheres. In an in vitro NCC culture, Prok-1 was able to activate both Akt and MAPK pathways and induce the proliferation and differentiation (but not migration) of NCCs via PK-R1. Knock-down of PK-R1 using siRNA resulted in a complete abolishment of Prok-1 induced proliferation. Taken together, it is the first report demonstrating that Prok-1 acts as a gut mucosa/mesenchyme-derived factor and maintains proliferation and differentiation of enteric NCCs. © 2007 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/bbamcren_HK
dc.relation.ispartofBiochimica et Biophysica Acta - Molecular Cell Researchen_HK
dc.rightsBiochimica et Biophysica Acta. Copyright © Elsevier BV.en_HK
dc.subjectDifferentiationen_HK
dc.subjectNeural crest cellen_HK
dc.subjectProkineticin-1en_HK
dc.subjectProliferationen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Differentiationen_HK
dc.subject.meshCell Movementen_HK
dc.subject.meshCell Proliferationen_HK
dc.subject.meshGastric Mucosa - cytology - metabolismen_HK
dc.subject.meshGastrointestinal Tract - cytology - embryology - metabolismen_HK
dc.subject.meshGene Expression Regulationen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMesoderm - cytology - metabolismen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred ICRen_HK
dc.subject.meshMitogen-Activated Protein Kinases - metabolismen_HK
dc.subject.meshNeural Crest - cytology - metabolismen_HK
dc.subject.meshPhosphatidylinositol 3-Kinases - metabolismen_HK
dc.subject.meshPhosphorylationen_HK
dc.subject.meshProtein Transporten_HK
dc.subject.meshProto-Oncogene Proteins c-akt - metabolismen_HK
dc.subject.meshReceptors, G-Protein-Coupled - genetics - metabolismen_HK
dc.subject.meshVascular Endothelial Growth Factor, Endocrine-Gland-Derived - genetics - metabolismen_HK
dc.titleProkineticin-1 modulates proliferation and differentiation of enteric neural crest cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-3002&volume=1773&issue=4&spage=536&epage=545&date=2007&atitle=Prokineticin-1+modulates+proliferation+and+differentiation+of+enteric+neural+crest+cellsen_HK
dc.identifier.emailNgan, ESW: engan@hkucc.hku.hken_HK
dc.identifier.emailSham, MH: mhsham@hkucc.hku.hken_HK
dc.identifier.emailLui, VCH: vchlui@hkucc.hku.hken_HK
dc.identifier.emailTam, PKH: paultam@hkucc.hku.hken_HK
dc.identifier.authorityNgan, ESW=rp00422en_HK
dc.identifier.authoritySham, MH=rp00380en_HK
dc.identifier.authorityLui, VCH=rp00363en_HK
dc.identifier.authorityTam, PKH=rp00060en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.bbamcr.2007.01.013en_HK
dc.identifier.pmid17324478-
dc.identifier.scopuseid_2-s2.0-33947427173en_HK
dc.identifier.hkuros126888en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33947427173&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1773en_HK
dc.identifier.issue4en_HK
dc.identifier.spage536en_HK
dc.identifier.epage545en_HK
dc.identifier.isiWOS:000245784700008-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridNgan, ESW=22234827500en_HK
dc.identifier.scopusauthoridLee, KY=10340983900en_HK
dc.identifier.scopusauthoridSit, FYL=16025577300en_HK
dc.identifier.scopusauthoridPoon, HC=35084222000en_HK
dc.identifier.scopusauthoridChan, JKY=15730226000en_HK
dc.identifier.scopusauthoridSham, MH=7003729109en_HK
dc.identifier.scopusauthoridLui, VCH=7004231344en_HK
dc.identifier.scopusauthoridTam, PKH=7202539421en_HK

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