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Article: Cleavage of spike protein of SARS coronavirus by protease factor Xa is associated with viral infectivity

TitleCleavage of spike protein of SARS coronavirus by protease factor Xa is associated with viral infectivity
Authors
KeywordsCleavage of S protein
Protease Factor Xa
Protease inhibitor
SARS-CoV
Spike protein
Issue Date2007
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2007, v. 359 n. 1, p. 174-179 How to Cite?
AbstractThe spike (S) protein of SARS coronavirus (SARS-CoV) has been known to recognize and bind to host receptors, whose conformational changes then facilitate fusion between the viral envelope and host cell membrane, leading to viral entry into target cells. However, other functions of SARS-CoV S protein such as proteolytic cleavage and its implications to viral infection are incompletely understood. In this study, we demonstrated that the infection of SARS-CoV and a pseudovirus bearing the S protein of SARS-CoV was inhibited by a protease inhibitor Ben-HCl. Also, the protease Factor Xa, a target of Ben-HCl abundantly expressed in infected cells, was able to cleave the recombinant and pseudoviral S protein into S1 and S2 subunits, and the cleavage was inhibited by Ben-HCl. Furthermore, this cleavage correlated with the infectivity of the pseudovirus. Taken together, our study suggests a plausible mechanism by which SARS-CoV cleaves its S protein to facilitate viral infection. © 2007 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/68160
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDu, Len_HK
dc.contributor.authorKao, RYen_HK
dc.contributor.authorZhou, Yen_HK
dc.contributor.authorHe, Yen_HK
dc.contributor.authorZhao, Gen_HK
dc.contributor.authorWong, Cen_HK
dc.contributor.authorJiang, Sen_HK
dc.contributor.authorYuen, KYen_HK
dc.contributor.authorJin, DYen_HK
dc.contributor.authorZheng, BJen_HK
dc.date.accessioned2010-09-06T06:01:56Z-
dc.date.available2010-09-06T06:01:56Z-
dc.date.issued2007en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2007, v. 359 n. 1, p. 174-179en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/68160-
dc.description.abstractThe spike (S) protein of SARS coronavirus (SARS-CoV) has been known to recognize and bind to host receptors, whose conformational changes then facilitate fusion between the viral envelope and host cell membrane, leading to viral entry into target cells. However, other functions of SARS-CoV S protein such as proteolytic cleavage and its implications to viral infection are incompletely understood. In this study, we demonstrated that the infection of SARS-CoV and a pseudovirus bearing the S protein of SARS-CoV was inhibited by a protease inhibitor Ben-HCl. Also, the protease Factor Xa, a target of Ben-HCl abundantly expressed in infected cells, was able to cleave the recombinant and pseudoviral S protein into S1 and S2 subunits, and the cleavage was inhibited by Ben-HCl. Furthermore, this cleavage correlated with the infectivity of the pseudovirus. Taken together, our study suggests a plausible mechanism by which SARS-CoV cleaves its S protein to facilitate viral infection. © 2007 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectCleavage of S proteinen_HK
dc.subjectProtease Factor Xaen_HK
dc.subjectProtease inhibitoren_HK
dc.subjectSARS-CoVen_HK
dc.subjectSpike proteinen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshFactor Xa - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshKidney - virologyen_HK
dc.subject.meshMembrane Glycoproteins - metabolismen_HK
dc.subject.meshProtein Bindingen_HK
dc.subject.meshViral Envelope Proteins - metabolismen_HK
dc.subject.meshVirus Activation - physiologyen_HK
dc.subject.meshVirus Replication - physiologyen_HK
dc.titleCleavage of spike protein of SARS coronavirus by protease factor Xa is associated with viral infectivityen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-291X&volume=359&issue=1&spage=174&epage=179.&date=2007&atitle=Cleavage+of+spike+protein+of+SARS+coronavirus+by+protease+factor+Xa+is+associated+with+viral+infectivity.en_HK
dc.identifier.emailKao, RY:rytkao@hkucc.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.emailJin, DY:dyjin@hkucc.hku.hken_HK
dc.identifier.emailZheng, BJ:bzheng@hkucc.hku.hken_HK
dc.identifier.authorityKao, RY=rp00481en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.identifier.authorityJin, DY=rp00452en_HK
dc.identifier.authorityZheng, BJ=rp00353en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.bbrc.2007.05.092en_HK
dc.identifier.pmid17533109-
dc.identifier.scopuseid_2-s2.0-34249740996en_HK
dc.identifier.hkuros132267en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34249740996&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume359en_HK
dc.identifier.issue1en_HK
dc.identifier.spage174en_HK
dc.identifier.epage179en_HK
dc.identifier.isiWOS:000247257600028-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridDu, L=8686996200en_HK
dc.identifier.scopusauthoridKao, RY=7101675499en_HK
dc.identifier.scopusauthoridZhou, Y=8791655300en_HK
dc.identifier.scopusauthoridHe, Y=8742157400en_HK
dc.identifier.scopusauthoridZhao, G=8684553000en_HK
dc.identifier.scopusauthoridWong, C=14824318400en_HK
dc.identifier.scopusauthoridJiang, S=7404453146en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.scopusauthoridJin, DY=7201973614en_HK
dc.identifier.scopusauthoridZheng, BJ=7201780588en_HK

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