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Article: The quantitative analysis of cyclin D1 expression in RA-treated and non-RA-treated ectomesenchymal cells in vitro

TitleThe quantitative analysis of cyclin D1 expression in RA-treated and non-RA-treated ectomesenchymal cells in vitro
Authors
Issue Date2000
PublisherSichuan Daxue, Huaxi Kouqiang Xueyuan. The Journal's web site is located at http://hxkqyxzz.periodicals.net.cn/
Citation
Hua Xi Kou Qiang Yi Xue Za Zhi = Huaxi Kouqiang Yixue Zazhi = West China Journal Of Stomatology, 2000, v. 18 n. 3, p. 150-152 How to Cite?
AbstractOBJECTIVE: To elucidate the alteration of Cyclin D1 protein between RA-treated and the non-RA-treated ectomesenchymal cells in embryonic mice. METHODS: Ectomesenchymal cells in the developing orofacial processes were explanted from embryonic BALB/c mice on generation day 12(15) (the 15th hour of the 12th day). Primary cells were cultured using the tissue mass method. The variants of growth curves were examined in the RA-treated and normal groups. Protein of Cyclin D1 was detected using immunohistochemistry. The positive degree of Cyclin D1 staining was analyzed using image analysis. RESULTS: The findings showed that the growth of ectomesenchymal cells was inhibited dramatically. Cyclin D1 expressed strongly in normal ectomesenchymal cells. However, its expression decreased evidently after RA-treated. There was significant difference between the test group and the normal group (P < 0.01). CONCLUSION: Low expression of Cyclin D1 may contribute to the inhibition of ectomesenchymal cells. It is necessary for Cyclin D1 to express appropriately during the normal meiosis of ectomesenchymal cells.
Persistent Identifierhttp://hdl.handle.net/10722/67912
ISSN
2015 SCImago Journal Rankings: 0.121

 

DC FieldValueLanguage
dc.contributor.authorLu, Hen_HK
dc.contributor.authorJin, Yen_HK
dc.contributor.authorTipoe, GLen_HK
dc.date.accessioned2010-09-06T05:59:24Z-
dc.date.available2010-09-06T05:59:24Z-
dc.date.issued2000en_HK
dc.identifier.citationHua Xi Kou Qiang Yi Xue Za Zhi = Huaxi Kouqiang Yixue Zazhi = West China Journal Of Stomatology, 2000, v. 18 n. 3, p. 150-152en_HK
dc.identifier.issn1000-1182en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67912-
dc.description.abstractOBJECTIVE: To elucidate the alteration of Cyclin D1 protein between RA-treated and the non-RA-treated ectomesenchymal cells in embryonic mice. METHODS: Ectomesenchymal cells in the developing orofacial processes were explanted from embryonic BALB/c mice on generation day 12(15) (the 15th hour of the 12th day). Primary cells were cultured using the tissue mass method. The variants of growth curves were examined in the RA-treated and normal groups. Protein of Cyclin D1 was detected using immunohistochemistry. The positive degree of Cyclin D1 staining was analyzed using image analysis. RESULTS: The findings showed that the growth of ectomesenchymal cells was inhibited dramatically. Cyclin D1 expressed strongly in normal ectomesenchymal cells. However, its expression decreased evidently after RA-treated. There was significant difference between the test group and the normal group (P < 0.01). CONCLUSION: Low expression of Cyclin D1 may contribute to the inhibition of ectomesenchymal cells. It is necessary for Cyclin D1 to express appropriately during the normal meiosis of ectomesenchymal cells.en_HK
dc.languageengen_HK
dc.publisherSichuan Daxue, Huaxi Kouqiang Xueyuan. The Journal's web site is located at http://hxkqyxzz.periodicals.net.cn/en_HK
dc.relation.ispartofHua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Culture Techniquesen_HK
dc.subject.meshCell Divisionen_HK
dc.subject.meshCyclin D1 - biosynthesisen_HK
dc.subject.meshEmbryo, Mammalianen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshMiceen_HK
dc.subject.meshPalate - embryologyen_HK
dc.subject.meshPregnancyen_HK
dc.subject.meshTretinoin - pharmacologyen_HK
dc.titleThe quantitative analysis of cyclin D1 expression in RA-treated and non-RA-treated ectomesenchymal cells in vitroen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1000-1182&volume=18&issue=3&spage=150&epage=152&date=2000&atitle=The+quantitative+analysis+of+cyclin+D1+expression+in+RA-treated+and+non-RA-treated+ectomesenchymal+cells+in+vitroen_HK
dc.identifier.emailTipoe, GL:tgeorge@hkucc.hku.hken_HK
dc.identifier.authorityTipoe, GL=rp00371en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.pmid12539663-
dc.identifier.scopuseid_2-s2.0-0037714392en_HK
dc.identifier.hkuros54608en_HK
dc.identifier.hkuros74735-
dc.identifier.volume18en_HK
dc.identifier.issue3en_HK
dc.identifier.spage150en_HK
dc.identifier.epage152en_HK
dc.publisher.placeChinaen_HK
dc.identifier.scopusauthoridLu, H=7404843896en_HK
dc.identifier.scopusauthoridJin, Y=26643271200en_HK
dc.identifier.scopusauthoridTipoe, GL=7003550610en_HK

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