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Article: NADPH-diaphorase neurons in the retina of the hamster

TitleNADPH-diaphorase neurons in the retina of the hamster
Authors
Issue Date1994
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31248
Citation
Journal Of Comparative Neurology, 1994, v. 350 n. 4, p. 550-558 How to Cite?
AbstractNADPH-diaphorase-positive neurons have been demonstrated in the inner nuclear layer and ganglion cell layer of the retina of different mammalian species, but so far no experiments have been conducted to identify whether these cells are amacrine cells and/or retinal ganglion cells. We attempted to solve this problem by studying the NADPH-diaphorase-positive neurons in the hamster retina. From the NADPH-diaphorase histochemical reaction, two distinct types of neurons in the hamster retina were identified. They were named ND(g) and ND(i) cells. The ND(g) cells were cells with larger somata, ranging from 10 to 21 μm in diameter with a mean of 15.58 μm (S.D. = 2.59). They were found in the ganglion cell layer only. The ND(i) cells were smaller, with the somata ranging from 7 to 11 μm and having the mean diameter of 8.77 μm (S.D. = 1.24). Most of the ND(i) cells were found in the inner nuclear layer, and only very few could be observed in the inner plexiform layer. On average, there were 8,033 ND(g) and 5,051 ND(i) cells in the ganglion cell layer and inner nuclear layer, respectively. Two experiments were performed to clarify whether any of the NADPH-diaphorase neurons were retinal ganglion cells. Following unilateral optic nerve section, which leads to the retrograde degeneration of retinal ganglion cells, the numbers of both ND(g) and ND(i) cells did not change significantly for up to 4 months. In addition, when retinal ganglion cells were prelabeled retrogradely (horseradish peroxidase or fluorescent microspheres) and retinas were then stained for NADPH diaphorase, no double-labeled neurons were detected. These results indicated that the NADPH-diaphorase neurons in the hamster retina were the amacrine cells in the inner nuclear layer and displaced amacrine cells in the ganglion cell layer. Dendrites of the ND(g) and ND(i) cells were found to stratify in sublaminae 1, 3, and 5 of the inner plexiform layer, with a prominent staining in the sublamina 5. The possible importance of this arrangement in the rod pathway is also discussed.
Persistent Identifierhttp://hdl.handle.net/10722/67455
ISSN
2015 Impact Factor: 3.331
2015 SCImago Journal Rankings: 2.345
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorKam Cheung Lauen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorTay, Den_HK
dc.contributor.authorLeung, MCPen_HK
dc.date.accessioned2010-09-06T05:55:17Z-
dc.date.available2010-09-06T05:55:17Z-
dc.date.issued1994en_HK
dc.identifier.citationJournal Of Comparative Neurology, 1994, v. 350 n. 4, p. 550-558en_HK
dc.identifier.issn0021-9967en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67455-
dc.description.abstractNADPH-diaphorase-positive neurons have been demonstrated in the inner nuclear layer and ganglion cell layer of the retina of different mammalian species, but so far no experiments have been conducted to identify whether these cells are amacrine cells and/or retinal ganglion cells. We attempted to solve this problem by studying the NADPH-diaphorase-positive neurons in the hamster retina. From the NADPH-diaphorase histochemical reaction, two distinct types of neurons in the hamster retina were identified. They were named ND(g) and ND(i) cells. The ND(g) cells were cells with larger somata, ranging from 10 to 21 μm in diameter with a mean of 15.58 μm (S.D. = 2.59). They were found in the ganglion cell layer only. The ND(i) cells were smaller, with the somata ranging from 7 to 11 μm and having the mean diameter of 8.77 μm (S.D. = 1.24). Most of the ND(i) cells were found in the inner nuclear layer, and only very few could be observed in the inner plexiform layer. On average, there were 8,033 ND(g) and 5,051 ND(i) cells in the ganglion cell layer and inner nuclear layer, respectively. Two experiments were performed to clarify whether any of the NADPH-diaphorase neurons were retinal ganglion cells. Following unilateral optic nerve section, which leads to the retrograde degeneration of retinal ganglion cells, the numbers of both ND(g) and ND(i) cells did not change significantly for up to 4 months. In addition, when retinal ganglion cells were prelabeled retrogradely (horseradish peroxidase or fluorescent microspheres) and retinas were then stained for NADPH diaphorase, no double-labeled neurons were detected. These results indicated that the NADPH-diaphorase neurons in the hamster retina were the amacrine cells in the inner nuclear layer and displaced amacrine cells in the ganglion cell layer. Dendrites of the ND(g) and ND(i) cells were found to stratify in sublaminae 1, 3, and 5 of the inner plexiform layer, with a prominent staining in the sublamina 5. The possible importance of this arrangement in the rod pathway is also discussed.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/31248en_HK
dc.relation.ispartofJournal of Comparative Neurologyen_HK
dc.subject.meshAmino Acid Oxidoreductases - metabolismen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCricetinaeen_HK
dc.subject.meshDendrites - enzymology - ultrastructureen_HK
dc.subject.meshHorseradish Peroxidaseen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMesocricetusen_HK
dc.subject.meshMicroscopy, Fluorescenceen_HK
dc.subject.meshMicrospheresen_HK
dc.subject.meshNADPH Dehydrogenase - metabolismen_HK
dc.subject.meshNerve Degeneration - physiologyen_HK
dc.subject.meshNeurons - enzymologyen_HK
dc.subject.meshNitric Oxide Synthaseen_HK
dc.subject.meshOptic Nerve - physiologyen_HK
dc.subject.meshRetina - cytology - enzymologyen_HK
dc.subject.meshRetinal Ganglion Cells - enzymologyen_HK
dc.titleNADPH-diaphorase neurons in the retina of the hamsteren_HK
dc.typeArticleen_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailTay, D:dkctay@hkucc.hku.hken_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityTay, D=rp00336en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/cne.903500404en_HK
dc.identifier.pmid7534316-
dc.identifier.scopuseid_2-s2.0-0028131572en_HK
dc.identifier.hkuros4615en_HK
dc.identifier.volume350en_HK
dc.identifier.issue4en_HK
dc.identifier.spage550en_HK
dc.identifier.epage558en_HK
dc.identifier.isiWOS:A1994QF84900003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKam Cheung Lau=7409804682en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK
dc.identifier.scopusauthoridTay, D=7006796825en_HK
dc.identifier.scopusauthoridLeung, MCP=7201943351en_HK

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