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Article: Up-regulated endogenous erythropoietin/erythropoietin receptor system and exogenous erythropoietin rescue retinal ganglion cells after chronic ocular hypertension

TitleUp-regulated endogenous erythropoietin/erythropoietin receptor system and exogenous erythropoietin rescue retinal ganglion cells after chronic ocular hypertension
Authors
Issue Date2008
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0272-4340
Citation
Cellular And Molecular Neurobiology, 2008, v. 28 n. 2, p. 317-329 How to Cite?
AbstractAims: Recent studies have showed that erythropoietin (EPO) is a neuroprotectant for central nerve system neurons in addition to being a hematopoietic cytokine in response to hypoxia. In this study, we investigate the role of the EPO/EPO receptor (EPOR) system in the rat retina after ocular hypertension injury that mimics glaucoma. Methods: Elevated intraocular pressure was induced by laser coagulation of the episcleral and limbal veins. Expression of EPO and EPOR in the normal and glaucomous retinas was investigated by immunohistochemistry and Western blot. To examine the effects of endogenous EPO on the survival of retinal ganglion cells (RGCs) subjected to hypertensive injury, soluble EPOR was directly injected into the vitreous body. Recombinant human EPO was both intravitreally and systemically administrated to study the effect of exogenous EPO on the survival of RGCs after ocular hypertension injury. Results: Immunohistochemistry studies identified Müller cells as the main source of EPO in the normal retina. Expression of EPO and EPOR proteins was increased significantly 2 weeks after ocular hypertension. RGCs, amacrine and bipolar cells all demonstrated an increased expression of EPOR after ocular hypertension. Neutralization of endogenous EPO with soluble EPOR exacerbated ocular hypertensive injury, suggesting a role of the EPO/EPOR system in the survival of RGCs after injury. Similarly, topical and systemic administration of recombinant human EPO rescues RGCs after chronic ocular hypertension. Conclusions: These results indicate that an endogenous EPO/EPOR system participates in intrinsic recovery mechanisms after retina injury and RGCs might be rescued by exogenous administration of EPO. © 2007 Springer Science+Business Media, LLC.
Persistent Identifierhttp://hdl.handle.net/10722/67420
ISSN
2015 Impact Factor: 2.328
2015 SCImago Journal Rankings: 1.005
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorFu, QLen_HK
dc.contributor.authorWu, Wen_HK
dc.contributor.authorWang, Hen_HK
dc.contributor.authorLi, Xen_HK
dc.contributor.authorLee, VWHen_HK
dc.contributor.authorSo, KFen_HK
dc.date.accessioned2010-09-06T05:54:59Z-
dc.date.available2010-09-06T05:54:59Z-
dc.date.issued2008en_HK
dc.identifier.citationCellular And Molecular Neurobiology, 2008, v. 28 n. 2, p. 317-329en_HK
dc.identifier.issn0272-4340en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67420-
dc.description.abstractAims: Recent studies have showed that erythropoietin (EPO) is a neuroprotectant for central nerve system neurons in addition to being a hematopoietic cytokine in response to hypoxia. In this study, we investigate the role of the EPO/EPO receptor (EPOR) system in the rat retina after ocular hypertension injury that mimics glaucoma. Methods: Elevated intraocular pressure was induced by laser coagulation of the episcleral and limbal veins. Expression of EPO and EPOR in the normal and glaucomous retinas was investigated by immunohistochemistry and Western blot. To examine the effects of endogenous EPO on the survival of retinal ganglion cells (RGCs) subjected to hypertensive injury, soluble EPOR was directly injected into the vitreous body. Recombinant human EPO was both intravitreally and systemically administrated to study the effect of exogenous EPO on the survival of RGCs after ocular hypertension injury. Results: Immunohistochemistry studies identified Müller cells as the main source of EPO in the normal retina. Expression of EPO and EPOR proteins was increased significantly 2 weeks after ocular hypertension. RGCs, amacrine and bipolar cells all demonstrated an increased expression of EPOR after ocular hypertension. Neutralization of endogenous EPO with soluble EPOR exacerbated ocular hypertensive injury, suggesting a role of the EPO/EPOR system in the survival of RGCs after injury. Similarly, topical and systemic administration of recombinant human EPO rescues RGCs after chronic ocular hypertension. Conclusions: These results indicate that an endogenous EPO/EPOR system participates in intrinsic recovery mechanisms after retina injury and RGCs might be rescued by exogenous administration of EPO. © 2007 Springer Science+Business Media, LLC.en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0272-4340en_HK
dc.relation.ispartofCellular and Molecular Neurobiologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Survival - drug effectsen_HK
dc.subject.meshChronic Diseaseen_HK
dc.subject.meshErythropoietin - metabolism - pharmacologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshNeuroprotective Agents - pharmacologyen_HK
dc.subject.meshOcular Hypertension - drug therapy - metabolism - pathologyen_HK
dc.subject.meshOptic Nerve Injuriesen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshReceptors, Erythropoietin - metabolismen_HK
dc.subject.meshRetinal Ganglion Cells - cytology - drug effects - metabolismen_HK
dc.subject.meshSolubilityen_HK
dc.subject.meshUp-Regulation - physiologyen_HK
dc.titleUp-regulated endogenous erythropoietin/erythropoietin receptor system and exogenous erythropoietin rescue retinal ganglion cells after chronic ocular hypertensionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1672-7681&volume=28&spage=317&epage=329&date=2008&atitle=Up-regulated+endogenous+erythropoietin/erythropoietin+receptor+system+and+exogenous+erythropoietin+rescue+retinal+ganglion+cells+after+chronic+ocular+hypertensionen_HK
dc.identifier.emailWu, W:wtwu@hkucc.hku.hken_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.authorityWu, W=rp00419en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s10571-007-9155-zen_HK
dc.identifier.pmid17554621en_HK
dc.identifier.scopuseid_2-s2.0-39149092649en_HK
dc.identifier.hkuros141223en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-39149092649&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume28en_HK
dc.identifier.issue2en_HK
dc.identifier.spage317en_HK
dc.identifier.epage329en_HK
dc.identifier.isiWOS:000253191900012-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridFu, QL=23388762000en_HK
dc.identifier.scopusauthoridWu, W=7407081122en_HK
dc.identifier.scopusauthoridWang, H=23502392800en_HK
dc.identifier.scopusauthoridLi, X=12139563100en_HK
dc.identifier.scopusauthoridLee, VWH=7402507569en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK

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