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Article: Inhibitor of apoptosis proteins and ovarian dysfunction in galactosemic rats

TitleInhibitor of apoptosis proteins and ovarian dysfunction in galactosemic rats
Authors
KeywordsApoptosis
Galactosemia
Granulosa cells
Inhibitor of apoptosis proteins
Ovulation
Rat (Sprague Dawley)
Issue Date2003
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htm
Citation
Cell And Tissue Research, 2003, v. 311 n. 3, p. 417-425 How to Cite?
AbstractGalactosemia is a genetic disease with deficiency of galactose-1-uridyltransferase, resulting in the accumulation of galactose or galactose-1-phosphate in the blood and tissues. Rats were fed with normal rat chow and with a high-galactose diet for 4 weeks to give control and galactosemic groups, and their ovarian function was studied. The two groups of rats were injected with pregnant mare's serum gonadotrophin (PMSG) and were killed at different time points after human chorionic gonadotrophin (hCG) injection. The number of oocytes ovulated in the controls was significantly higher than in the galactosemic group. Morphometric studies of the ovaries also showed a higher number of corpora lutea in the controls. Western blot analysis of granulosa cells showed that the overall expressions of Fas and FasL were lower in the control group and their expressions of inhibitor of apoptosis proteins (IAPs) were higher than in the galactosemic group, especially at 8 h post hCG injection. TDT-mediated dUTP-biotin nick end-labeling (TUNEL) and immunohistochemical staining of ovarian sections with Ki-67 and IAPs showed more apoptotic granulosa cells in the galactosemic group and the expressions of IAPs in granulosa cells also confirmed the result of the Western blot. These findings support our hypothesis that ovarian dysfunction in galactosemic rats is due to increased apoptosis in granulosa cells of maturing follicles.
Persistent Identifierhttp://hdl.handle.net/10722/67411
ISSN
2015 Impact Factor: 2.948
2015 SCImago Journal Rankings: 1.539
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLai, KWen_HK
dc.contributor.authorCheng, LYLen_HK
dc.contributor.authorCheung, ALMen_HK
dc.contributor.authorO, WSen_HK
dc.date.accessioned2010-09-06T05:54:54Z-
dc.date.available2010-09-06T05:54:54Z-
dc.date.issued2003en_HK
dc.identifier.citationCell And Tissue Research, 2003, v. 311 n. 3, p. 417-425en_HK
dc.identifier.issn0302-766Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/67411-
dc.description.abstractGalactosemia is a genetic disease with deficiency of galactose-1-uridyltransferase, resulting in the accumulation of galactose or galactose-1-phosphate in the blood and tissues. Rats were fed with normal rat chow and with a high-galactose diet for 4 weeks to give control and galactosemic groups, and their ovarian function was studied. The two groups of rats were injected with pregnant mare's serum gonadotrophin (PMSG) and were killed at different time points after human chorionic gonadotrophin (hCG) injection. The number of oocytes ovulated in the controls was significantly higher than in the galactosemic group. Morphometric studies of the ovaries also showed a higher number of corpora lutea in the controls. Western blot analysis of granulosa cells showed that the overall expressions of Fas and FasL were lower in the control group and their expressions of inhibitor of apoptosis proteins (IAPs) were higher than in the galactosemic group, especially at 8 h post hCG injection. TDT-mediated dUTP-biotin nick end-labeling (TUNEL) and immunohistochemical staining of ovarian sections with Ki-67 and IAPs showed more apoptotic granulosa cells in the galactosemic group and the expressions of IAPs in granulosa cells also confirmed the result of the Western blot. These findings support our hypothesis that ovarian dysfunction in galactosemic rats is due to increased apoptosis in granulosa cells of maturing follicles.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00441/index.htmen_HK
dc.relation.ispartofCell and Tissue Researchen_HK
dc.subjectApoptosisen_HK
dc.subjectGalactosemiaen_HK
dc.subjectGranulosa cellsen_HK
dc.subjectInhibitor of apoptosis proteinsen_HK
dc.subjectOvulationen_HK
dc.subjectRat (Sprague Dawley)en_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAntigens, CD95 - metabolismen_HK
dc.subject.meshApoptosis - physiologyen_HK
dc.subject.meshCell Survival - physiologyen_HK
dc.subject.meshChorionic Gonadotropin - pharmacologyen_HK
dc.subject.meshDisease Models, Animalen_HK
dc.subject.meshFas Ligand Proteinen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshFood, Formulated - adverse effectsen_HK
dc.subject.meshGalactose - adverse effects - metabolismen_HK
dc.subject.meshGalactosemias - complications - metabolism - pathologyen_HK
dc.subject.meshGonadotropins, Equine - pharmacologyen_HK
dc.subject.meshGranulosa Cells - metabolism - pathologyen_HK
dc.subject.meshInhibitor of Apoptosis Proteinsen_HK
dc.subject.meshMembrane Glycoproteins - metabolismen_HK
dc.subject.meshOvarian Diseases - etiology - metabolism - pathologyen_HK
dc.subject.meshProteins - metabolismen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.titleInhibitor of apoptosis proteins and ovarian dysfunction in galactosemic ratsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0302-766X&volume=311&spage=417&epage=425&date=2003&atitle=Inhibitor+of+apoptosis+proteins+and+ovarian+dysfunction+in+galactosemic+ratsen_HK
dc.identifier.emailCheng, LYL:lcheng@hkucc.hku.hken_HK
dc.identifier.emailCheung, ALM:lmcheung@hkucc.hku.hken_HK
dc.identifier.emailO, WS:owaisum@hkucc.hku.hken_HK
dc.identifier.authorityCheng, LYL=rp00317en_HK
dc.identifier.authorityCheung, ALM=rp00332en_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00441-002-0689-6-
dc.identifier.pmid12658449-
dc.identifier.scopuseid_2-s2.0-0037365289en_HK
dc.identifier.hkuros76297en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037365289&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume311en_HK
dc.identifier.issue3en_HK
dc.identifier.spage417en_HK
dc.identifier.epage425en_HK
dc.identifier.isiWOS:000182409600015-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridLai, KW=7402135541en_HK
dc.identifier.scopusauthoridCheng, LYL=7403337122en_HK
dc.identifier.scopusauthoridCheung, ALM=7401806497en_HK
dc.identifier.scopusauthoridO, WS=6701729369en_HK

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