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Article: Interaction between SARS-CoV helicase and a multifunctional cellular protein (Ddx5) revealed by yeast and mammalian cell two-hybrid systems

TitleInteraction between SARS-CoV helicase and a multifunctional cellular protein (Ddx5) revealed by yeast and mammalian cell two-hybrid systems
Authors
Issue Date2009
PublisherSpringer Wien. The Journal's web site is located at http://www.springer.com/springerwiennewyork/medicine/journal/705
Citation
Archives Of Virology, 2009, v. 154 n. 3, p. 507-512 How to Cite?
AbstractTo reveal the putative cellular factors involved in SARS coronavirus replication, the helicase (Hel, nsp13) of SARS coronavirus was used to screen the cDNA library of rat pulmonary epithelial cells using the yeast two-hybrid system. Positively interacting proteins were further tested using a mammalian cell hybrid system and co-immunoprecipitation in the human A549 cell line, which has been shown to support SARS coronavirus replication. Out of the seven positive clones observed by yeast two-hybrid assay, only the Ddx5 (Asp-Glu-Ala-Asp box polypeptide 5) protein showed specific interaction with SARS-CoV helicase. When expression of DdX5 was knocked down by small interfering RNA (siRNA), SARS coronavirus replication was significantly inhibited in fetal rhesus kidney (FRhK-4) cells. Since Ddx5 is a multifunctional protein that plays important roles in transcriptional regulation, its interaction with SARS coronavirus helicase provides interesting clues for studying virus-host cell interactions in SARS-CoV infections. © 2009 Springer-Verlag.
Persistent Identifierhttp://hdl.handle.net/10722/59384
ISSN
2014 Impact Factor: 2.390
ISI Accession Number ID
Funding AgencyGrant Number
Sino-GermanGZ230 (202/3)
Funding Information:

This work was supported by Sino-German collaborative grant GZ230 (202/3).

References

 

DC FieldValueLanguage
dc.contributor.authorChen, JYen_HK
dc.contributor.authorChen, WNen_HK
dc.contributor.authorPoon, KMVen_HK
dc.contributor.authorZheng, BJen_HK
dc.contributor.authorLin, Xen_HK
dc.contributor.authorWang, YXen_HK
dc.contributor.authorWen, YMen_HK
dc.date.accessioned2010-05-31T03:49:00Z-
dc.date.available2010-05-31T03:49:00Z-
dc.date.issued2009en_HK
dc.identifier.citationArchives Of Virology, 2009, v. 154 n. 3, p. 507-512en_HK
dc.identifier.issn0304-8608en_HK
dc.identifier.urihttp://hdl.handle.net/10722/59384-
dc.description.abstractTo reveal the putative cellular factors involved in SARS coronavirus replication, the helicase (Hel, nsp13) of SARS coronavirus was used to screen the cDNA library of rat pulmonary epithelial cells using the yeast two-hybrid system. Positively interacting proteins were further tested using a mammalian cell hybrid system and co-immunoprecipitation in the human A549 cell line, which has been shown to support SARS coronavirus replication. Out of the seven positive clones observed by yeast two-hybrid assay, only the Ddx5 (Asp-Glu-Ala-Asp box polypeptide 5) protein showed specific interaction with SARS-CoV helicase. When expression of DdX5 was knocked down by small interfering RNA (siRNA), SARS coronavirus replication was significantly inhibited in fetal rhesus kidney (FRhK-4) cells. Since Ddx5 is a multifunctional protein that plays important roles in transcriptional regulation, its interaction with SARS coronavirus helicase provides interesting clues for studying virus-host cell interactions in SARS-CoV infections. © 2009 Springer-Verlag.en_HK
dc.languageengen_HK
dc.publisherSpringer Wien. The Journal's web site is located at http://www.springer.com/springerwiennewyork/medicine/journal/705en_HK
dc.relation.ispartofArchives of Virologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshDEAD-box RNA Helicases - metabolismen_HK
dc.subject.meshGene Knockdown Techniquesen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMacaca mulattaen_HK
dc.subject.meshMethyltransferases - metabolismen_HK
dc.subject.meshProtein Bindingen_HK
dc.subject.meshProtein Interaction Mappingen_HK
dc.subject.meshRatsen_HK
dc.subject.meshSARS Virus - physiologyen_HK
dc.subject.meshTwo-Hybrid System Techniquesen_HK
dc.subject.meshVirus Replicationen_HK
dc.titleInteraction between SARS-CoV helicase and a multifunctional cellular protein (Ddx5) revealed by yeast and mammalian cell two-hybrid systemsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0304-8608&volume=154&issue=3&spage=507&epage=512&date=2009&atitle=Interaction+between+SARS-CoV+helicase+and+a+multifunctional+cellular+protein+(Ddx5)+revealed+by+yeast+and+mammalian+cell+two-hybrid+systems.++en_HK
dc.identifier.emailZheng, BJ:bzheng@hkucc.hku.hken_HK
dc.identifier.authorityZheng, BJ=rp00353en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00705-009-0323-yen_HK
dc.identifier.pmid19224332en_HK
dc.identifier.scopuseid_2-s2.0-62149134190en_HK
dc.identifier.hkuros156486en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-62149134190&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume154en_HK
dc.identifier.issue3en_HK
dc.identifier.spage507en_HK
dc.identifier.epage512en_HK
dc.identifier.isiWOS:000263915400015-
dc.publisher.placeAustriaen_HK
dc.identifier.scopusauthoridChen, JY=25621052600en_HK
dc.identifier.scopusauthoridChen, WN=8715827200en_HK
dc.identifier.scopusauthoridPoon, KMV=36776892700en_HK
dc.identifier.scopusauthoridZheng, BJ=7201780588en_HK
dc.identifier.scopusauthoridLin, X=24729123000en_HK
dc.identifier.scopusauthoridWang, YX=9942220100en_HK
dc.identifier.scopusauthoridWen, YM=7401776949en_HK
dc.identifier.citeulike4088662-

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