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Article: Interaction between SARS-CoV helicase and a multifunctional cellular protein (Ddx5) revealed by yeast and mammalian cell two-hybrid systems
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TitleInteraction between SARS-CoV helicase and a multifunctional cellular protein (Ddx5) revealed by yeast and mammalian cell two-hybrid systems
 
AuthorsChen, JY2
Chen, WN2
Poon, KMV1
Zheng, BJ1
Lin, X2
Wang, YX3
Wen, YM3
 
Issue Date2009
 
PublisherSpringer Wien. The Journal's web site is located at http://www.springer.com/springerwiennewyork/medicine/journal/705
 
CitationArchives Of Virology, 2009, v. 154 n. 3, p. 507-512 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00705-009-0323-y
 
AbstractTo reveal the putative cellular factors involved in SARS coronavirus replication, the helicase (Hel, nsp13) of SARS coronavirus was used to screen the cDNA library of rat pulmonary epithelial cells using the yeast two-hybrid system. Positively interacting proteins were further tested using a mammalian cell hybrid system and co-immunoprecipitation in the human A549 cell line, which has been shown to support SARS coronavirus replication. Out of the seven positive clones observed by yeast two-hybrid assay, only the Ddx5 (Asp-Glu-Ala-Asp box polypeptide 5) protein showed specific interaction with SARS-CoV helicase. When expression of DdX5 was knocked down by small interfering RNA (siRNA), SARS coronavirus replication was significantly inhibited in fetal rhesus kidney (FRhK-4) cells. Since Ddx5 is a multifunctional protein that plays important roles in transcriptional regulation, its interaction with SARS coronavirus helicase provides interesting clues for studying virus-host cell interactions in SARS-CoV infections. © 2009 Springer-Verlag.
 
ISSN0304-8608
2013 Impact Factor: 2.282
 
DOIhttp://dx.doi.org/10.1007/s00705-009-0323-y
 
ISI Accession Number IDWOS:000263915400015
Funding AgencyGrant Number
Sino-GermanGZ230 (202/3)
Funding Information:

This work was supported by Sino-German collaborative grant GZ230 (202/3).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorChen, JY
 
dc.contributor.authorChen, WN
 
dc.contributor.authorPoon, KMV
 
dc.contributor.authorZheng, BJ
 
dc.contributor.authorLin, X
 
dc.contributor.authorWang, YX
 
dc.contributor.authorWen, YM
 
dc.date.accessioned2010-05-31T03:49:00Z
 
dc.date.available2010-05-31T03:49:00Z
 
dc.date.issued2009
 
dc.description.abstractTo reveal the putative cellular factors involved in SARS coronavirus replication, the helicase (Hel, nsp13) of SARS coronavirus was used to screen the cDNA library of rat pulmonary epithelial cells using the yeast two-hybrid system. Positively interacting proteins were further tested using a mammalian cell hybrid system and co-immunoprecipitation in the human A549 cell line, which has been shown to support SARS coronavirus replication. Out of the seven positive clones observed by yeast two-hybrid assay, only the Ddx5 (Asp-Glu-Ala-Asp box polypeptide 5) protein showed specific interaction with SARS-CoV helicase. When expression of DdX5 was knocked down by small interfering RNA (siRNA), SARS coronavirus replication was significantly inhibited in fetal rhesus kidney (FRhK-4) cells. Since Ddx5 is a multifunctional protein that plays important roles in transcriptional regulation, its interaction with SARS coronavirus helicase provides interesting clues for studying virus-host cell interactions in SARS-CoV infections. © 2009 Springer-Verlag.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationArchives Of Virology, 2009, v. 154 n. 3, p. 507-512 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00705-009-0323-y
 
dc.identifier.citeulike4088662
 
dc.identifier.doihttp://dx.doi.org/10.1007/s00705-009-0323-y
 
dc.identifier.epage512
 
dc.identifier.hkuros156486
 
dc.identifier.isiWOS:000263915400015
Funding AgencyGrant Number
Sino-GermanGZ230 (202/3)
Funding Information:

This work was supported by Sino-German collaborative grant GZ230 (202/3).

 
dc.identifier.issn0304-8608
2013 Impact Factor: 2.282
 
dc.identifier.issue3
 
dc.identifier.openurl
 
dc.identifier.pmid19224332
 
dc.identifier.scopuseid_2-s2.0-62149134190
 
dc.identifier.spage507
 
dc.identifier.urihttp://hdl.handle.net/10722/59384
 
dc.identifier.volume154
 
dc.languageeng
 
dc.publisherSpringer Wien. The Journal's web site is located at http://www.springer.com/springerwiennewyork/medicine/journal/705
 
dc.publisher.placeAustria
 
dc.relation.ispartofArchives of Virology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshCell Line
 
dc.subject.meshDEAD-box RNA Helicases - metabolism
 
dc.subject.meshGene Knockdown Techniques
 
dc.subject.meshHumans
 
dc.subject.meshMacaca mulatta
 
dc.subject.meshMethyltransferases - metabolism
 
dc.subject.meshProtein Binding
 
dc.subject.meshProtein Interaction Mapping
 
dc.subject.meshRats
 
dc.subject.meshSARS Virus - physiology
 
dc.subject.meshTwo-Hybrid System Techniques
 
dc.subject.meshVirus Replication
 
dc.titleInteraction between SARS-CoV helicase and a multifunctional cellular protein (Ddx5) revealed by yeast and mammalian cell two-hybrid systems
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Fujian Medical University
  3. Fudan University Shanghai Medical College