Article: Interaction between SARS-CoV helicase and a multifunctional cellular protein (Ddx5) revealed by yeast and mammalian cell two-hybrid systems

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TitleInteraction between SARS-CoV helicase and a multifunctional cellular protein (Ddx5) revealed by yeast and mammalian cell two-hybrid systems
AuthorsChen, JY2
Chen, WN2
Poon, KMV1
Zheng, BJ1
Lin, X2
Wang, YX3
Wen, YM3
Issue Date2009
PublisherSpringer Wien. The Journal's web site is located at http://www.springer.com/springerwiennewyork/medicine/journal/705
CitationArchives Of Virology, 2009, v. 154 n. 3, p. 507-512 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00705-009-0323-y
AbstractTo reveal the putative cellular factors involved in SARS coronavirus replication, the helicase (Hel, nsp13) of SARS coronavirus was used to screen the cDNA library of rat pulmonary epithelial cells using the yeast two-hybrid system. Positively interacting proteins were further tested using a mammalian cell hybrid system and co-immunoprecipitation in the human A549 cell line, which has been shown to support SARS coronavirus replication. Out of the seven positive clones observed by yeast two-hybrid assay, only the Ddx5 (Asp-Glu-Ala-Asp box polypeptide 5) protein showed specific interaction with SARS-CoV helicase. When expression of DdX5 was knocked down by small interfering RNA (siRNA), SARS coronavirus replication was significantly inhibited in fetal rhesus kidney (FRhK-4) cells. Since Ddx5 is a multifunctional protein that plays important roles in transcriptional regulation, its interaction with SARS coronavirus helicase provides interesting clues for studying virus-host cell interactions in SARS-CoV infections. © 2009 Springer-Verlag.
ISSN0304-8608
2011 Impact Factor: 2.111
2011 SCImago Journal Rankings: 0.162
DOIhttp://dx.doi.org/10.1007/s00705-009-0323-y
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorChen, JY
dc.contributor.authorChen, WN
dc.contributor.authorPoon, KMV
dc.contributor.authorZheng, BJ
dc.contributor.authorLin, X
dc.contributor.authorWang, YX
dc.contributor.authorWen, YM
dc.date.accessioned2010-05-31T03:49:00Z
dc.date.available2010-05-31T03:49:00Z
dc.date.issued2009
dc.description.abstractTo reveal the putative cellular factors involved in SARS coronavirus replication, the helicase (Hel, nsp13) of SARS coronavirus was used to screen the cDNA library of rat pulmonary epithelial cells using the yeast two-hybrid system. Positively interacting proteins were further tested using a mammalian cell hybrid system and co-immunoprecipitation in the human A549 cell line, which has been shown to support SARS coronavirus replication. Out of the seven positive clones observed by yeast two-hybrid assay, only the Ddx5 (Asp-Glu-Ala-Asp box polypeptide 5) protein showed specific interaction with SARS-CoV helicase. When expression of DdX5 was knocked down by small interfering RNA (siRNA), SARS coronavirus replication was significantly inhibited in fetal rhesus kidney (FRhK-4) cells. Since Ddx5 is a multifunctional protein that plays important roles in transcriptional regulation, its interaction with SARS coronavirus helicase provides interesting clues for studying virus-host cell interactions in SARS-CoV infections. © 2009 Springer-Verlag.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationArchives Of Virology, 2009, v. 154 n. 3, p. 507-512 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s00705-009-0323-y
dc.identifier.citeulike4088662
dc.identifier.doihttp://dx.doi.org/10.1007/s00705-009-0323-y
dc.identifier.epage512
dc.identifier.hkuros156486
dc.identifier.isiWOS:000263915400015
Funding AgencyGrant Number
Sino-GermanGZ230 (202/3)
Funding Information:

This work was supported by Sino-German collaborative grant GZ230 (202/3).

dc.identifier.issn0304-8608
2011 Impact Factor: 2.111
2011 SCImago Journal Rankings: 0.162
dc.identifier.issue3
dc.identifier.openurl
dc.identifier.pmid19224332
dc.identifier.scopuseid_2-s2.0-62149134190
dc.identifier.spage507
dc.identifier.urihttp://hdl.handle.net/10722/59384
dc.identifier.volume154
dc.languageeng
dc.publisherSpringer Wien. The Journal's web site is located at http://www.springer.com/springerwiennewyork/medicine/journal/705
dc.publisher.placeAustria
dc.relation.ispartofArchives of Virology
dc.relation.referencesReferences in Scopus
dc.subject.meshAnimals
dc.subject.meshCell Line
dc.subject.meshDEAD-box RNA Helicases - metabolism
dc.subject.meshGene Knockdown Techniques
dc.subject.meshHumans
dc.subject.meshMacaca mulatta
dc.subject.meshMethyltransferases - metabolism
dc.subject.meshProtein Binding
dc.subject.meshProtein Interaction Mapping
dc.subject.meshRats
dc.subject.meshSARS Virus - physiology
dc.subject.meshTwo-Hybrid System Techniques
dc.subject.meshVirus Replication
dc.titleInteraction between SARS-CoV helicase and a multifunctional cellular protein (Ddx5) revealed by yeast and mammalian cell two-hybrid systems
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Fujian Medical University
  3. Fudan University Shanghai Medical College