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Article: Synergistic activity of lysozyme and antifungal agents against Candida albicans biofilms on denture acrylic surfaces

TitleSynergistic activity of lysozyme and antifungal agents against Candida albicans biofilms on denture acrylic surfaces
Authors
Issue Date2009
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio
Citation
Archives Of Oral Biology, 2009, v. 54 n. 2, p. 115-126 How to Cite?
AbstractDenture related oral candidiasis is a recalcitrant fungal infection not easily resolved by topical antifungals. The antimycotic protein lysozyme, in saliva is an important host defense mechanism although its activity against Candida biofilms on denture acrylic has not been evaluated. Objectives: (i) To establish a clinically relevant denture acrylic assay model to develop standardized Candida albicans biofilms, and (ii) assess the inhibitory effects of lysozyme alone and, the latter combined with antifungals (nystatin, amphotericin B, ketoconazole and 5-fluorocytosine) on sessile Candida cells and, finally (iii) to visualize the accompanying ultrastructural changes. Design: The rotating-disc biofilm reactor was used to develop standardized 48 h Candida biofilms on acrylic discs in YNB/100 mM glucose medium and the biofilm metabolic activity was monitored using a tetrazolium reduction assay. Results: The biofilm metabolic activity was similar in 18 identical denture acrylic discs (p < 0.05) thus validating the rotating-disc biofilm model. Very low concentrations of lysozyme (6.25 μg/ml) significantly (p < 0.01) inhibited Candida biofilm formation indicating that lysozyme may likely regulate intra-oral Candida biofilm development. Although 100 μg/ml lysozyme killed 45% of sessile Candida cells, further increasing its concentration (up to 240 μg/ml) had no such effect. Nystatin, amphotericin B, and ketoconazole in association with 100 μg/ml lysozyme exhibited effective synergistic killing of biofilm Candida in comparison to drug-free controls. Scanning electron and confocal scanning laser microscopy analysis confirmed the latter trends. Conclusion: Our results indicate that agents found in biological fluids such as lysozyme could be a safe adjunct to antifungals in future treatment strategies for recalcitrant candidal infections. © 2008 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/58123
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.713
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSamaranayake, YHen_HK
dc.contributor.authorCheung, BPKen_HK
dc.contributor.authorParahitiyawa, Nen_HK
dc.contributor.authorSeneviratne, CJen_HK
dc.contributor.authorYau, JYYen_HK
dc.contributor.authorYeung, KWSen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2010-05-31T03:24:11Z-
dc.date.available2010-05-31T03:24:11Z-
dc.date.issued2009en_HK
dc.identifier.citationArchives Of Oral Biology, 2009, v. 54 n. 2, p. 115-126en_HK
dc.identifier.issn0003-9969en_HK
dc.identifier.urihttp://hdl.handle.net/10722/58123-
dc.description.abstractDenture related oral candidiasis is a recalcitrant fungal infection not easily resolved by topical antifungals. The antimycotic protein lysozyme, in saliva is an important host defense mechanism although its activity against Candida biofilms on denture acrylic has not been evaluated. Objectives: (i) To establish a clinically relevant denture acrylic assay model to develop standardized Candida albicans biofilms, and (ii) assess the inhibitory effects of lysozyme alone and, the latter combined with antifungals (nystatin, amphotericin B, ketoconazole and 5-fluorocytosine) on sessile Candida cells and, finally (iii) to visualize the accompanying ultrastructural changes. Design: The rotating-disc biofilm reactor was used to develop standardized 48 h Candida biofilms on acrylic discs in YNB/100 mM glucose medium and the biofilm metabolic activity was monitored using a tetrazolium reduction assay. Results: The biofilm metabolic activity was similar in 18 identical denture acrylic discs (p < 0.05) thus validating the rotating-disc biofilm model. Very low concentrations of lysozyme (6.25 μg/ml) significantly (p < 0.01) inhibited Candida biofilm formation indicating that lysozyme may likely regulate intra-oral Candida biofilm development. Although 100 μg/ml lysozyme killed 45% of sessile Candida cells, further increasing its concentration (up to 240 μg/ml) had no such effect. Nystatin, amphotericin B, and ketoconazole in association with 100 μg/ml lysozyme exhibited effective synergistic killing of biofilm Candida in comparison to drug-free controls. Scanning electron and confocal scanning laser microscopy analysis confirmed the latter trends. Conclusion: Our results indicate that agents found in biological fluids such as lysozyme could be a safe adjunct to antifungals in future treatment strategies for recalcitrant candidal infections. © 2008 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbioen_HK
dc.relation.ispartofArchives of Oral Biologyen_HK
dc.subject.meshAcrylic Resinsen_HK
dc.subject.meshAntifungal Agents - pharmacologyen_HK
dc.subject.meshBiofilms - drug effects - growth & developmenten_HK
dc.subject.meshCandida albicans - drug effects - growth & development - ultrastructureen_HK
dc.subject.meshDenture Bases - microbiologyen_HK
dc.subject.meshDose-Response Relationship, Drugen_HK
dc.subject.meshDrug Combinationsen_HK
dc.subject.meshDrug Evaluation, Preclinical - methodsen_HK
dc.subject.meshDrug Synergismen_HK
dc.subject.meshEquipment Contaminationen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMicrobial Sensitivity Tests - methodsen_HK
dc.subject.meshMicroscopy, Confocalen_HK
dc.subject.meshMicroscopy, Electron, Scanningen_HK
dc.subject.meshMuramidase - pharmacologyen_HK
dc.titleSynergistic activity of lysozyme and antifungal agents against Candida albicans biofilms on denture acrylic surfacesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0003-9969&volume=54&spage=115&epage=126&date=2009&atitle=Synergistic+activity+of+lysozyme+and+antifungal+agents+against+Candida+albicans+biofilms+on+denture+acrylic+surfacesen_HK
dc.identifier.emailSamaranayake, YH:hema@hkucc.hku.hken_HK
dc.identifier.emailSeneviratne, CJ:jaya@hku.hken_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySamaranayake, YH=rp00025en_HK
dc.identifier.authoritySeneviratne, CJ=rp01372en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.archoralbio.2008.09.015en_HK
dc.identifier.pmid19038377-
dc.identifier.scopuseid_2-s2.0-57549097610en_HK
dc.identifier.hkuros155260en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-57549097610&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume54en_HK
dc.identifier.issue2en_HK
dc.identifier.spage115en_HK
dc.identifier.epage126en_HK
dc.identifier.isiWOS:000263189600003-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridSamaranayake, YH=6602677237en_HK
dc.identifier.scopusauthoridCheung, BPK=7103294773en_HK
dc.identifier.scopusauthoridParahitiyawa, N=13408948800en_HK
dc.identifier.scopusauthoridSeneviratne, CJ=6701897753en_HK
dc.identifier.scopusauthoridYau, JYY=7102167568en_HK
dc.identifier.scopusauthoridYeung, KWS=25931923800en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK

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