File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Altered expression and deletion of RMO1 in osteosarcoma

TitleAltered expression and deletion of RMO1 in osteosarcoma
Authors
KeywordsLoss of heterozygosity
Metastasis
mRNA expression
Osteosarcoma
RMO1
Issue Date2005
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
Citation
International Journal Of Cancer, 2005, v. 114 n. 5, p. 738-746 How to Cite?
Abstract
In order to increase our understanding of the molecular events underlying osteosarcoma progression, the expression of approximately 950 genes was examined in 24 primary and metastatic osteosarcoma tumor specimens. A gene, RMO1, was isolated with decreased expression in metastatic samples. Real-Time PCR corroborated this pattern, revealing lower expression in the primary sample in 6 of 7 cases for which both primary and metastatic osteosarcoma samples were available from the same patient (p = 0.034). RMO1 is located at 2q33, a region of frequent loss of heterozygosity in cancer, and exhibited loss of heterozygosity in 6 out of 9 primary osteosarcoma tumor samples (67%). Loss of heterozygosity is evident in primary tumors while the decrease in gene expression is seen in the metastatic samples, indicating that these 2 events are separately implicated in cancer progression. Cloning of RMO1 revealed an open reading frame with multiple splice forms with significant homology to GRB7, 10 and 14 and MIG10 in the region containing a Pleckstrin homology domain and a Ras association domain, suggestive of a role in cell signaling and migration. Northern blot analysis indicated that RMO1 mRNA is ubiquitously expressed in tissues except for peripheral blood leukocytes. These data suggest that RMO1 may be a candidate for a protein involved in inhibiting tumor progression. © 2004 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/44391
ISSN
2013 Impact Factor: 5.007
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorEppert, Ken_HK
dc.contributor.authorWunder, JSen_HK
dc.contributor.authorAneliunas, Ven_HK
dc.contributor.authorTsui, LCen_HK
dc.contributor.authorScherer, SWen_HK
dc.contributor.authorAndrulis, ILen_HK
dc.date.accessioned2007-09-12T03:52:38Z-
dc.date.available2007-09-12T03:52:38Z-
dc.date.issued2005en_HK
dc.identifier.citationInternational Journal Of Cancer, 2005, v. 114 n. 5, p. 738-746en_HK
dc.identifier.issn0020-7136en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44391-
dc.description.abstractIn order to increase our understanding of the molecular events underlying osteosarcoma progression, the expression of approximately 950 genes was examined in 24 primary and metastatic osteosarcoma tumor specimens. A gene, RMO1, was isolated with decreased expression in metastatic samples. Real-Time PCR corroborated this pattern, revealing lower expression in the primary sample in 6 of 7 cases for which both primary and metastatic osteosarcoma samples were available from the same patient (p = 0.034). RMO1 is located at 2q33, a region of frequent loss of heterozygosity in cancer, and exhibited loss of heterozygosity in 6 out of 9 primary osteosarcoma tumor samples (67%). Loss of heterozygosity is evident in primary tumors while the decrease in gene expression is seen in the metastatic samples, indicating that these 2 events are separately implicated in cancer progression. Cloning of RMO1 revealed an open reading frame with multiple splice forms with significant homology to GRB7, 10 and 14 and MIG10 in the region containing a Pleckstrin homology domain and a Ras association domain, suggestive of a role in cell signaling and migration. Northern blot analysis indicated that RMO1 mRNA is ubiquitously expressed in tissues except for peripheral blood leukocytes. These data suggest that RMO1 may be a candidate for a protein involved in inhibiting tumor progression. © 2004 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/homeen_HK
dc.relation.ispartofInternational Journal of Canceren_HK
dc.subjectLoss of heterozygosityen_HK
dc.subjectMetastasisen_HK
dc.subjectmRNA expressionen_HK
dc.subjectOsteosarcomaen_HK
dc.subjectRMO1en_HK
dc.subject.meshMedical sciences - oncologyen_HK
dc.subject.meshMetastasisen_HK
dc.subject.meshRmo1en_HK
dc.subject.meshMrna expressionen_HK
dc.subject.meshLoss of heterozygosityen_HK
dc.titleAltered expression and deletion of RMO1 in osteosarcomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0020-7136&volume=114&issue=5&spage=738&epage=746&date=2005&atitle=Altered+expression+and+deletion+of+RMO1+in+osteosarcomaen_HK
dc.identifier.emailTsui, LC: tsuilc@hkucc.hku.hken_HK
dc.identifier.authorityTsui, LC=rp00058en_HK
dc.description.natureabstracten_HK
dc.identifier.doi10.1002/ijc.20786en_HK
dc.identifier.pmid15609301en_HK
dc.identifier.scopuseid_2-s2.0-14844311294en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-14844311294&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume114en_HK
dc.identifier.issue5en_HK
dc.identifier.spage738en_HK
dc.identifier.epage746en_HK
dc.identifier.isiWOS:000227535600008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridEppert, K=8307015700en_HK
dc.identifier.scopusauthoridWunder, JS=7004882354en_HK
dc.identifier.scopusauthoridAneliunas, V=6505848558en_HK
dc.identifier.scopusauthoridTsui, LC=7102754167en_HK
dc.identifier.scopusauthoridScherer, SW=35374654500en_HK
dc.identifier.scopusauthoridAndrulis, IL=7005655442en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats