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Article: Introduction and recovery of a selectable bacterial gene from the genome of mammalian cells

TitleIntroduction and recovery of a selectable bacterial gene from the genome of mammalian cells
Authors
Issue Date1982
PublisherAmerican Society for Microbiology.
Citation
Molecular and cellular biology, 1982, v. 2 n. 8, p. 966-976 How to Cite?
AbstractThe simian virus 40 (SV40)-pBR322 recombinant, pSV2, carrying the origin of SV40 replication and the gpt gene of Escherichia coli, has been stably introduced into Chinese hamster ovary hprt- cells. All gpt-transformed cell lines were found to contain one or more insertions of pSV2 sequences exclusively associated with high-molecular-weight DNA. Additional analyses showed that at least one integrated copy in each cell line retained an intact gpt gene and flanking SV40 sequences required for expression of xanthine-guanine phosphoribosyltransferase. Most cell lines contained pSV2 sequences which had integrated with partial sequence duplication. Upon fusion with COS-1 cells, a simian cell line permissive for autonomous pSV2 replication, most gpt-transformed cell lines produced low-molecular-weight DNA molecules related to pSV2. The majority of these replicating DNAs were indistinguishable from the original transfecting plasmid in both size and restriction enzyme cleavage pattern. In addition, the recovered DNA molecules were able to confer ampicillin resistance to E. coli and to transform mouse L cells and Gpt- E. coli to a Gpt+ phenotype. These studies indicate that all of the genetic information carried by this SV40-plasmid recombinant can be introduced into and retrieved from the genome of mammalian cells.
Persistent Identifierhttp://hdl.handle.net/10722/44203
ISSN
2015 Impact Factor: 4.427
2015 SCImago Journal Rankings: 3.806
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PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBreitman, MLen_HK
dc.contributor.authorTsui, L-Cen_HK
dc.contributor.authorBuchwald, Men_HK
dc.contributor.authorSiminovitch, Len_HK
dc.date.accessioned2007-09-12T03:48:56Z-
dc.date.available2007-09-12T03:48:56Z-
dc.date.issued1982en_HK
dc.identifierhttp://mcb.asm.org/cgi/reprint/2/8/966.pdfen_HK
dc.identifier.citationMolecular and cellular biology, 1982, v. 2 n. 8, p. 966-976en_HK
dc.identifier.issn0270-7306en_HK
dc.identifier.urihttp://hdl.handle.net/10722/44203-
dc.description.abstractThe simian virus 40 (SV40)-pBR322 recombinant, pSV2, carrying the origin of SV40 replication and the gpt gene of Escherichia coli, has been stably introduced into Chinese hamster ovary hprt- cells. All gpt-transformed cell lines were found to contain one or more insertions of pSV2 sequences exclusively associated with high-molecular-weight DNA. Additional analyses showed that at least one integrated copy in each cell line retained an intact gpt gene and flanking SV40 sequences required for expression of xanthine-guanine phosphoribosyltransferase. Most cell lines contained pSV2 sequences which had integrated with partial sequence duplication. Upon fusion with COS-1 cells, a simian cell line permissive for autonomous pSV2 replication, most gpt-transformed cell lines produced low-molecular-weight DNA molecules related to pSV2. The majority of these replicating DNAs were indistinguishable from the original transfecting plasmid in both size and restriction enzyme cleavage pattern. In addition, the recovered DNA molecules were able to confer ampicillin resistance to E. coli and to transform mouse L cells and Gpt- E. coli to a Gpt+ phenotype. These studies indicate that all of the genetic information carried by this SV40-plasmid recombinant can be introduced into and retrieved from the genome of mammalian cells.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology.en_HK
dc.relation.ispartofMolecular and cellular biology-
dc.rightsCopyright © American Society for Microbiology, Molecular and cellular biology, 1982, v. 2 n. 8, p. 966-976en_HK
dc.subject.meshCricetinaeen_HK
dc.subject.meshCricetulusen_HK
dc.subject.meshDna restriction enzymesen_HK
dc.subject.meshDna, recombinanten_HK
dc.subject.meshEscherichia coli - geneticsen_HK
dc.titleIntroduction and recovery of a selectable bacterial gene from the genome of mammalian cellsen_HK
dc.typeArticleen_HK
dc.description.naturelink_to_OA_fulltexten_HK
dc.identifier.pmid6290875-
dc.identifier.pmcidPMC369884-
dc.identifier.scopuseid_2-s2.0-0020322353-
dc.identifier.isiWOS:A1982PA31300010-

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