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Article: Stem Cells from Human Exfoliated Deciduous Teeth (SHEDs) and Dental Pulp Stem Cells (DPSCs) Display a Similar Profile with Pericytes

TitleStem Cells from Human Exfoliated Deciduous Teeth (SHEDs) and Dental Pulp Stem Cells (DPSCs) Display a Similar Profile with Pericytes
Authors
Editors
Editor(s):Mussano, F
Issue Date2021
PublisherHindawi Publishing Corporation. The Journal's web site is located at http://www.sage-hindawi.com/journals/sci/
Citation
Stem Cells International, 2021, v. 2021, p. article no. 8859902 How to Cite?
AbstractBackground: Pericytes play an important role in forming functional blood vessels and establishing stable and effective microcirculation, which is crucial for vascular tissue engineering. The slow ex vivo expansion rate, limited proliferative capacity, and variability of tissue-specific phenotypes would hinder experimental studies and clinical translation of primary pericytes. In this study, the angiogenic and pericyte functions of stem cells from human exfoliated deciduous teeth (SHEDs) and postnatal human dental pulp stem cells (DPSCs) were investigated. Methods: Osteogenic and adipogenic induction assays were performed to evaluate the mesenchymal potential of SHEDs, DPSCs, and pericytes. An in vitro Matrigel angiogenesis assay was conducted to reveal the ability of SHEDs, DPSCs, and pericytes to stabilize vascular-like structures. Quantitative real-time polymerase chain reaction (RT-qPCR) was performed to evaluate mRNA expression. Flow cytometry, western blotting, and immunostaining were used to assess the protein expression. Wound healing and transwell assays were performed to evaluate the migration ability of SHEDs, DPSCs, and pericytes. Results: The osteogenic and adipogenic induction assays showed that SHEDs, DPSCs, and pericytes exhibited similar stem cell characteristics. The mRNA expression levels of PDGFR-β, α-SMA, NG2, and DEMSIN in SHEDs and DPSCs cultured in EC medium were significantly higher than those in the control groups on day 7 (P < 0.05), but significantly higher than those in the pericytes group on day 14 (P < 0.05). Flow cytometry showed that high proportions of SHEDs and DPSCs were positive for various pericyte markers on day 7. The DPSCs, SHEDs, and pericytes displayed strong migration ability; however, there was no significant difference among the groups (P > 0.05). Conclusion: The SHEDs and DPSCs display a profile similar to that of pericytes. Our study lays a solid theoretical foundation for the clinical use of dental pulp stem cells as a potential candidate to replace pericytes. Copyright © 2021 Shao Yue Zhu et al.
Persistent Identifierhttp://hdl.handle.net/10722/306479
ISSN
2020 Impact Factor: 5.443
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZHU, SY-
dc.contributor.authorYuan, CY-
dc.contributor.authorLin, YF-
dc.contributor.authorLiu, H-
dc.contributor.authorYang, YQ-
dc.contributor.authorWong, HM-
dc.contributor.authorZhang, CF-
dc.contributor.authorWang, PL-
dc.contributor.authorGu, M-
dc.contributor.editorMussano, F-
dc.date.accessioned2021-10-22T07:35:12Z-
dc.date.available2021-10-22T07:35:12Z-
dc.date.issued2021-
dc.identifier.citationStem Cells International, 2021, v. 2021, p. article no. 8859902-
dc.identifier.issn1687-966X-
dc.identifier.urihttp://hdl.handle.net/10722/306479-
dc.description.abstractBackground: Pericytes play an important role in forming functional blood vessels and establishing stable and effective microcirculation, which is crucial for vascular tissue engineering. The slow ex vivo expansion rate, limited proliferative capacity, and variability of tissue-specific phenotypes would hinder experimental studies and clinical translation of primary pericytes. In this study, the angiogenic and pericyte functions of stem cells from human exfoliated deciduous teeth (SHEDs) and postnatal human dental pulp stem cells (DPSCs) were investigated. Methods: Osteogenic and adipogenic induction assays were performed to evaluate the mesenchymal potential of SHEDs, DPSCs, and pericytes. An in vitro Matrigel angiogenesis assay was conducted to reveal the ability of SHEDs, DPSCs, and pericytes to stabilize vascular-like structures. Quantitative real-time polymerase chain reaction (RT-qPCR) was performed to evaluate mRNA expression. Flow cytometry, western blotting, and immunostaining were used to assess the protein expression. Wound healing and transwell assays were performed to evaluate the migration ability of SHEDs, DPSCs, and pericytes. Results: The osteogenic and adipogenic induction assays showed that SHEDs, DPSCs, and pericytes exhibited similar stem cell characteristics. The mRNA expression levels of PDGFR-β, α-SMA, NG2, and DEMSIN in SHEDs and DPSCs cultured in EC medium were significantly higher than those in the control groups on day 7 (P < 0.05), but significantly higher than those in the pericytes group on day 14 (P < 0.05). Flow cytometry showed that high proportions of SHEDs and DPSCs were positive for various pericyte markers on day 7. The DPSCs, SHEDs, and pericytes displayed strong migration ability; however, there was no significant difference among the groups (P > 0.05). Conclusion: The SHEDs and DPSCs display a profile similar to that of pericytes. Our study lays a solid theoretical foundation for the clinical use of dental pulp stem cells as a potential candidate to replace pericytes. Copyright © 2021 Shao Yue Zhu et al.-
dc.languageeng-
dc.publisherHindawi Publishing Corporation. The Journal's web site is located at http://www.sage-hindawi.com/journals/sci/-
dc.relation.ispartofStem Cells International-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleStem Cells from Human Exfoliated Deciduous Teeth (SHEDs) and Dental Pulp Stem Cells (DPSCs) Display a Similar Profile with Pericytes-
dc.typeArticle-
dc.identifier.emailLin, YF: yflin@hku.hk-
dc.identifier.emailYang, YQ: yangyanq@hku.hk-
dc.identifier.emailWong, HM: wonghmg@hkucc.hku.hk-
dc.identifier.emailZhang, CF: zhangcf@hku.hk-
dc.identifier.emailGu, M: drgumin@hku.hk-
dc.identifier.authorityLin, YF=rp02516-
dc.identifier.authorityYang, YQ=rp00045-
dc.identifier.authorityWong, HM=rp00042-
dc.identifier.authorityZhang, CF=rp01408-
dc.identifier.authorityGu, M=rp01892-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1155/2021/8859902-
dc.identifier.pmid34349804-
dc.identifier.pmcidPMC8328701-
dc.identifier.hkuros329068-
dc.identifier.hkuros330357-
dc.identifier.volume2021-
dc.identifier.spagearticle no. 8859902-
dc.identifier.epagearticle no. 8859902-
dc.identifier.isiWOS:000684843900001-
dc.publisher.placeUnited States-

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