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Article: Reprogramming Porcine Fibroblast to EPSCs

TitleReprogramming Porcine Fibroblast to EPSCs
Authors
Gao, XRuan, DLiu, P
KeywordsPorcine
Expanded potential stem cells
Induced pluripotent stem cell
Reprogramming
Issue Date2021
PublisherHumana Press, Inc. The Journal's web site is located at http://link.springer.com/bookseries/7651
Citation
Methods in Molecular Biology, 2021, v. 2239, p. 199-211 How to Cite?
DOI: http://dx.doi.org/10.1007/978-1-0716-1084-8_13
AbstractThe development of porcine expanded potential stem cells (pEPSCs) provides an invaluable tool for investigation of porcine stem cell pluripotency and opens a venue for research in biotechnology, agriculture, and regenerative medicine. Since the derivation of pEPSC from porcine pre-implantation embryos has been demanding in resource supply and technical challenges, it is more feasible and convenient for most laboratories to derive this new type of porcine stem cells by reprogramming somatic cells. In this chapter, we describe the detailed procedures for reprogramming porcine fetal fibroblast cells to EPSCiPSC with the eight reprogramming factors cloned on the piggyBac vectors followed by a selection for pluripotent cells independent of transgene expression using the EPSC media. This technique allows the generation of pEPSCs for stem cell research, genome editing, biotechnology, and agriculture.
Persistent Identifierhttp://hdl.handle.net/10722/295898
ISSN
1064-3745
2020 SCImago Journal Rankings: 0.711

 

DC FieldValueLanguage
dc.contributor.authorGao, X-
dc.contributor.authorRuan, D-
dc.contributor.authorLiu, P-
dc.date.accessioned2021-02-08T08:15:36Z-
dc.date.available2021-02-08T08:15:36Z-
dc.date.issued2021-
dc.identifier.citationMethods in Molecular Biology, 2021, v. 2239, p. 199-211-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10722/295898-
dc.description.abstractThe development of porcine expanded potential stem cells (pEPSCs) provides an invaluable tool for investigation of porcine stem cell pluripotency and opens a venue for research in biotechnology, agriculture, and regenerative medicine. Since the derivation of pEPSC from porcine pre-implantation embryos has been demanding in resource supply and technical challenges, it is more feasible and convenient for most laboratories to derive this new type of porcine stem cells by reprogramming somatic cells. In this chapter, we describe the detailed procedures for reprogramming porcine fetal fibroblast cells to EPSCiPSC with the eight reprogramming factors cloned on the piggyBac vectors followed by a selection for pluripotent cells independent of transgene expression using the EPSC media. This technique allows the generation of pEPSCs for stem cell research, genome editing, biotechnology, and agriculture.-
dc.languageeng-
dc.publisherHumana Press, Inc. The Journal's web site is located at http://link.springer.com/bookseries/7651-
dc.relation.ispartofMethods in Molecular Biology-
dc.rightsThe original publication is available at www.springerlink.com-
dc.subjectPorcine-
dc.subjectExpanded potential stem cells-
dc.subjectInduced pluripotent stem cell-
dc.subjectReprogramming-
dc.titleReprogramming Porcine Fibroblast to EPSCs-
dc.typeArticle-
dc.identifier.emailRuan, D: degong77@hku.hk-
dc.identifier.emailLiu, P: pliu88@hku.hk-
dc.identifier.authorityLiu, P=rp02328-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/978-1-0716-1084-8_13-
dc.identifier.pmid33226621-
dc.identifier.scopuseid_2-s2.0-85096773785-
dc.identifier.hkuros321214-
dc.identifier.volume2239-
dc.identifier.spage199-
dc.identifier.epage211-
dc.publisher.placeUnited States-

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