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Book Chapter: Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-Induced Signalling in Cancer Cells

TitleAssessment of Stabilization and Activity of the HIFs Important for Hypoxia-Induced Signalling in Cancer Cells
Authors
KeywordsHypoxia
HIF
qPCR
Western blotting
Luciferase reporter assay
Issue Date2019
PublisherHumana Press
Citation
Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-Induced Signalling in Cancer Cells. In Haznadar, M (Ed.), Cancer Metabolism: Methods and Protocols, p. 77-99. New York, NY: Humana Press, 2019 How to Cite?
AbstractBlood vessels in tumors contain chaotic branching structures and leaky vessel lumens, resulting in uneven supply of oxygen in the tumor microenvironment. High metabolic and proliferation rate of tumor cells further depletes the local oxygen supply. Therefore, hypoxia is a common phenomenon in multiple solid malignancies. Hypoxia-inducible factors (HIFs) regulate the transcription of a spectrum of genes, which are vitally important for tumor cell adaption under hypoxia, and shape the tumor microenvironment to become more favorable for progression. HIFs are involved in almost every step of cancer development through inducing angiogenesis, metabolic reprogramming, metastasis, cancer stemness maintenance, chemoresistance, and immune evasion. Here, we describe methods for the assessment of HIF activity, as well as identification of novel transcriptional targets of HIFs in vitro and in vivo.
Persistent Identifierhttp://hdl.handle.net/10722/293722
ISBN
Series/Report no.Methods in Molecular Biology (MIMB) ; v. 1928

 

DC FieldValueLanguage
dc.contributor.authorChiu, DKC-
dc.contributor.authorZhang, S-
dc.contributor.authorTse, PW-
dc.contributor.authorWong, CCL-
dc.date.accessioned2020-11-23T08:20:53Z-
dc.date.available2020-11-23T08:20:53Z-
dc.date.issued2019-
dc.identifier.citationAssessment of Stabilization and Activity of the HIFs Important for Hypoxia-Induced Signalling in Cancer Cells. In Haznadar, M (Ed.), Cancer Metabolism: Methods and Protocols, p. 77-99. New York, NY: Humana Press, 2019-
dc.identifier.isbn9781493990269-
dc.identifier.urihttp://hdl.handle.net/10722/293722-
dc.description.abstractBlood vessels in tumors contain chaotic branching structures and leaky vessel lumens, resulting in uneven supply of oxygen in the tumor microenvironment. High metabolic and proliferation rate of tumor cells further depletes the local oxygen supply. Therefore, hypoxia is a common phenomenon in multiple solid malignancies. Hypoxia-inducible factors (HIFs) regulate the transcription of a spectrum of genes, which are vitally important for tumor cell adaption under hypoxia, and shape the tumor microenvironment to become more favorable for progression. HIFs are involved in almost every step of cancer development through inducing angiogenesis, metabolic reprogramming, metastasis, cancer stemness maintenance, chemoresistance, and immune evasion. Here, we describe methods for the assessment of HIF activity, as well as identification of novel transcriptional targets of HIFs in vitro and in vivo.-
dc.languageeng-
dc.publisherHumana Press-
dc.relation.ispartofCancer Metabolism: Methods and Protocols-
dc.relation.ispartofseriesMethods in Molecular Biology (MIMB) ; v. 1928-
dc.subjectHypoxia-
dc.subjectHIF-
dc.subjectqPCR-
dc.subjectWestern blotting-
dc.subjectLuciferase reporter assay-
dc.titleAssessment of Stabilization and Activity of the HIFs Important for Hypoxia-Induced Signalling in Cancer Cells-
dc.typeBook_Chapter-
dc.identifier.emailZhang, S: mistyzs@hku.hk-
dc.identifier.emailTse, PW: akipwtse@hku.hk-
dc.identifier.emailWong, CCL: carmencl@pathology.hku.hk-
dc.identifier.authorityWong, CCL=rp01602-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/978-1-4939-9027-6_6-
dc.identifier.pmid30725452-
dc.identifier.hkuros320031-
dc.identifier.spage77-
dc.identifier.epage99-
dc.publisher.placeNew York, NY-

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