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Article: Modulation of the mechanosensing of mesenchymal stem cells by laser-induced patterning for the acceleration of tissue reconstruction through the Wnt/β-catenin signaling pathway activation

TitleModulation of the mechanosensing of mesenchymal stem cells by laser-induced patterning for the acceleration of tissue reconstruction through the Wnt/β-catenin signaling pathway activation
Authors
KeywordsLaser
Mesenchymal stem cells
Signaling pathway
Implants
Mechanosensing
Issue Date2020
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/actabiomat
Citation
Acta Biomaterialia, 2020, v. 101, p. 152-167 How to Cite?
AbstractGrowing evidence suggests that the physical microenvironment can guide cell fate. However, cells sense cues from the adjacent physical microenvironment over a limited distance. In the present study, murine mesenchymal stem cells (MSCs) and murine preosteoblastic cells (MC3T3-E1) behaviors are regulated by the cell–material interface using ordered-micro and disordered-nano patterned structures on Ti implants. The optimal bone formation structure is a stable wave (horizontal direction: ridge, 2.7 µm; grooves, 5.3 µm; and vertical direction: distance, 700 µm) with the appropriate density of nano-branches (6.0 per µm2). The repeated waves provide cells with directional guidance, and the disordered branches influence cell geometry by providing different spacing and density nanostructure. And micro-nano patterned structure can provide biophysical cues to direct cell phenotype development, including cell size, shape, and orientation, to influence cellular processes including survival, growth, and differentiation. Thus, the overlaid isotropic and anisotropic cues, ordered-micro and disordered-nano patterned structures, could transfer further and alter cell shape and induce nuclear orientation by activating Wnt/β-catenin signaling to promote integrin α5, integrin β1, cadherin 2, Runx2, Opn, and Ocn. That canonical Wnt signaling inhibitor dickkopf1 further demonstrates osteogenic differentiation induced by ordered-micro and disordered-nano patterned structures, which is related to Wnt/β-catenin signaling. Our findings show the role of ordered microstructures and disordered nanostructures in modulating stem cell differentiation with potential medical applications. Statement of significance: It remains a challenge to modify poor osteogenic and osteoconductive properties of titanium alloy bases on the inherent poverty of titanium. We demonstrate that ordered microtopography and disordered nano topography pattern structure could lead to osteogenic differentiation in vitro and bone regeneration in vivo. Furthermore, the pattern structure is created through selective laser melting and alkali heat. And the structure only takes advantage of titanium itself and does not bring in active film, such as hydroxyapatite. On the other hand, we find that cell shape and orientation show angle-orientation tendency due to the polarity, which involves with mechanical signal created via patterned structure. Meanwhile, the Wnt/Ca2+ signaling pathway is activated.
Persistent Identifierhttp://hdl.handle.net/10722/289803
ISSN
2019 Impact Factor: 7.242
2015 SCImago Journal Rankings: 2.020

 

DC FieldValueLanguage
dc.contributor.authorFU, J-
dc.contributor.authorLIU, X-
dc.contributor.authorTAN, L-
dc.contributor.authorCUI, Z-
dc.contributor.authorLIANG, Y-
dc.contributor.authorLI, Z-
dc.contributor.authorZHU, S-
dc.contributor.authorZHENG, Y-
dc.contributor.authorYeung, KWK-
dc.contributor.authorCHU, PK-
dc.contributor.authorWU, S-
dc.date.accessioned2020-10-22T08:17:42Z-
dc.date.available2020-10-22T08:17:42Z-
dc.date.issued2020-
dc.identifier.citationActa Biomaterialia, 2020, v. 101, p. 152-167-
dc.identifier.issn1742-7061-
dc.identifier.urihttp://hdl.handle.net/10722/289803-
dc.description.abstractGrowing evidence suggests that the physical microenvironment can guide cell fate. However, cells sense cues from the adjacent physical microenvironment over a limited distance. In the present study, murine mesenchymal stem cells (MSCs) and murine preosteoblastic cells (MC3T3-E1) behaviors are regulated by the cell–material interface using ordered-micro and disordered-nano patterned structures on Ti implants. The optimal bone formation structure is a stable wave (horizontal direction: ridge, 2.7 µm; grooves, 5.3 µm; and vertical direction: distance, 700 µm) with the appropriate density of nano-branches (6.0 per µm2). The repeated waves provide cells with directional guidance, and the disordered branches influence cell geometry by providing different spacing and density nanostructure. And micro-nano patterned structure can provide biophysical cues to direct cell phenotype development, including cell size, shape, and orientation, to influence cellular processes including survival, growth, and differentiation. Thus, the overlaid isotropic and anisotropic cues, ordered-micro and disordered-nano patterned structures, could transfer further and alter cell shape and induce nuclear orientation by activating Wnt/β-catenin signaling to promote integrin α5, integrin β1, cadherin 2, Runx2, Opn, and Ocn. That canonical Wnt signaling inhibitor dickkopf1 further demonstrates osteogenic differentiation induced by ordered-micro and disordered-nano patterned structures, which is related to Wnt/β-catenin signaling. Our findings show the role of ordered microstructures and disordered nanostructures in modulating stem cell differentiation with potential medical applications. Statement of significance: It remains a challenge to modify poor osteogenic and osteoconductive properties of titanium alloy bases on the inherent poverty of titanium. We demonstrate that ordered microtopography and disordered nano topography pattern structure could lead to osteogenic differentiation in vitro and bone regeneration in vivo. Furthermore, the pattern structure is created through selective laser melting and alkali heat. And the structure only takes advantage of titanium itself and does not bring in active film, such as hydroxyapatite. On the other hand, we find that cell shape and orientation show angle-orientation tendency due to the polarity, which involves with mechanical signal created via patterned structure. Meanwhile, the Wnt/Ca2+ signaling pathway is activated.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/actabiomat-
dc.relation.ispartofActa Biomaterialia-
dc.subjectLaser-
dc.subjectMesenchymal stem cells-
dc.subjectSignaling pathway-
dc.subjectImplants-
dc.subjectMechanosensing-
dc.titleModulation of the mechanosensing of mesenchymal stem cells by laser-induced patterning for the acceleration of tissue reconstruction through the Wnt/β-catenin signaling pathway activation-
dc.typeArticle-
dc.identifier.emailYeung, KWK: wkkyeung@hku.hk-
dc.identifier.authorityYeung, KWK=rp00309-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.actbio.2019.10.041-
dc.identifier.pmid31678738-
dc.identifier.scopuseid_2-s2.0-85075460716-
dc.identifier.hkuros317536-
dc.identifier.volume101-
dc.identifier.spage152-
dc.identifier.epage167-
dc.publisher.placeNetherlands-

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