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Article: FRET-Mediated Observation of Protein-Triggered Conformational Changes in DNA Nanostructures

TitleFRET-Mediated Observation of Protein-Triggered Conformational Changes in DNA Nanostructures
Authors
KeywordsDNA origami
DNA–protein interaction
aptamers
molecular logic gate
Issue Date2020
PublisherHumana Press, Inc. The Journal's web site is located at http://link.springer.com/bookseries/7651
Citation
Methods in Molecular Biology, 2020, v. 2208, p. 69-80 How to Cite?
AbstractDNA origami is a powerful technique, which allows virtually limitless 2D or 3D nanostructure designs to be constructed from DNA strands. Such nanostructures can even include programmable nanorobots, which are able to respond to the environment in predetermined ways. DNA aptamers hold particular promise as interfaces, which can enable proteins, peptides, and other non-nucleic acid biomolecules to trigger conformational changes in DNA nanostructures for diagnostic, biosensing, or therapeutic applications. Here, we provide the methodology for FRET-mediated observation of aptamer-triggered conformational change in a DNA origami box nanostructure. The method described can, in principle, be adapted to a wide variety of experimental circumstances where the DNA nanostructure conformational change is mediated by molecular or environmental cues.
DescriptionCover title - Polypeptide Materials: Methods and Protocols
Persistent Identifierhttp://hdl.handle.net/10722/289587
ISBN
ISSN
2015 SCImago Journal Rankings: 0.549

 

DC FieldValueLanguage
dc.contributor.authorSHIU, SCC-
dc.contributor.authorSakai, Y-
dc.contributor.authorTanner, JA-
dc.contributor.authorHeddle, JG-
dc.date.accessioned2020-10-22T08:14:43Z-
dc.date.available2020-10-22T08:14:43Z-
dc.date.issued2020-
dc.identifier.citationMethods in Molecular Biology, 2020, v. 2208, p. 69-80-
dc.identifier.isbn9781071609279-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10722/289587-
dc.descriptionCover title - Polypeptide Materials: Methods and Protocols-
dc.description.abstractDNA origami is a powerful technique, which allows virtually limitless 2D or 3D nanostructure designs to be constructed from DNA strands. Such nanostructures can even include programmable nanorobots, which are able to respond to the environment in predetermined ways. DNA aptamers hold particular promise as interfaces, which can enable proteins, peptides, and other non-nucleic acid biomolecules to trigger conformational changes in DNA nanostructures for diagnostic, biosensing, or therapeutic applications. Here, we provide the methodology for FRET-mediated observation of aptamer-triggered conformational change in a DNA origami box nanostructure. The method described can, in principle, be adapted to a wide variety of experimental circumstances where the DNA nanostructure conformational change is mediated by molecular or environmental cues.-
dc.languageeng-
dc.publisherHumana Press, Inc. The Journal's web site is located at http://link.springer.com/bookseries/7651-
dc.relation.ispartofMethods in Molecular Biology-
dc.rightsThis is a post-peer-review, pre-copyedit version of an article published in [insert journal title]. The final authenticated version is available online at: https://doi.org/[insert DOI]-
dc.subjectDNA origami-
dc.subjectDNA–protein interaction-
dc.subjectaptamers-
dc.subjectmolecular logic gate-
dc.titleFRET-Mediated Observation of Protein-Triggered Conformational Changes in DNA Nanostructures-
dc.typeArticle-
dc.identifier.emailTanner, JA: jatanner@hkucc.hku.hk-
dc.identifier.authorityTanner, JA=rp00495-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/978-1-0716-0928-6_5-
dc.identifier.pmid32856256-
dc.identifier.scopuseid_2-s2.0-85090001181-
dc.identifier.hkuros316965-
dc.identifier.volume2208-
dc.identifier.spage69-
dc.identifier.epage80-
dc.publisher.placeUnited States-
dc.identifier.issnl1064-3745-

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