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Article: Identification of a Novel Ichthyic Parvovirus in Marine Species in Hainan Island, China

TitleIdentification of a Novel Ichthyic Parvovirus in Marine Species in Hainan Island, China
Authors
KeywordsParvoviridae
novel virus
Chaphamaparvovirus
crocodile
tilapia
Issue Date2019
PublisherFrontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/microbiology/
Citation
Frontiers in Microbiology, 2019, v. 10, p. article no. 2815 How to Cite?
AbstractParvoviruses are a diverse group of viruses that are capable of infecting a wide range of animals. In this study, we report the discovery of a novel parvovirus, tilapia parvovirus HMU-HKU, in the fecal samples of crocodiles and intestines of tilapia in Hainan Province, China. The novel parvovirus was firstly identified from crocodiles fed with tilapia using next-generation sequencing (NGS). Screening studies revealed that the prevalence of the novel parvovirus in crocodile feces samples fed on tilapia (75–86%) was apparently higher than that in crocodiles fed with chicken (4%). Further studies revealed that the prevalence of the novel parvovirus in tilapia feces samples collected at four areas in Hainan Province was between 40 and 90%. Four stains of the novel parvovirus were identified in this study based on sequence analyses of NS1 and all the four strains were found in tilapia in contrast only two of them were detected in crocodile feces. The nearly full-length genome sequence of the tilapia parvovirus HMU-HKU-1 was determined and showed less than 45.50 and 40.38% amino acid identity with other members of Parvoviridae in NS1 and VP1 genes, respectively. Phylogenetic analysis based on the complete helicase domain amino acid sequences showed that the tilapia parvovirus HMU-HKU-1 formed a relatively independent branch in the newly proposed genus Chaphamaparvovirus in the subfamily Hamaparvovirinae according to the ICTV’s most recent taxonomic criteria for Parvoviridae classification. Tilapia parvovirus HMU-HKU-1 likely represented a new species within the new genus Chaphamaparvovirus. The identification of tilapia parvovirus HMU-HKU provides further insight into the viral and genetic diversity of parvoviruses and its infections in tilapia populations need to be evaluated in terms of pathogenicity and production losses in tilapia farming.
Persistent Identifierhttp://hdl.handle.net/10722/289453
ISSN
2019 Impact Factor: 4.235
2015 SCImago Journal Rankings: 1.970
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorDu, J-
dc.contributor.authorWang, W-
dc.contributor.authorChan, JFW-
dc.contributor.authorWang, G-
dc.contributor.authorHuang, Y-
dc.contributor.authorYi, Y-
dc.contributor.authorZHU, Z-
dc.contributor.authorPeng, R-
dc.contributor.authorHu, X-
dc.contributor.authorWu, Y-
dc.contributor.authorZeng, J-
dc.contributor.authorZheng, J-
dc.contributor.authorCui, X-
dc.contributor.authorNiu, L-
dc.contributor.authorZhao, W-
dc.contributor.authorLu, G-
dc.contributor.authorYuen, KY-
dc.contributor.authorYin, F-
dc.date.accessioned2020-10-22T08:12:53Z-
dc.date.available2020-10-22T08:12:53Z-
dc.date.issued2019-
dc.identifier.citationFrontiers in Microbiology, 2019, v. 10, p. article no. 2815-
dc.identifier.issn1664-302X-
dc.identifier.urihttp://hdl.handle.net/10722/289453-
dc.description.abstractParvoviruses are a diverse group of viruses that are capable of infecting a wide range of animals. In this study, we report the discovery of a novel parvovirus, tilapia parvovirus HMU-HKU, in the fecal samples of crocodiles and intestines of tilapia in Hainan Province, China. The novel parvovirus was firstly identified from crocodiles fed with tilapia using next-generation sequencing (NGS). Screening studies revealed that the prevalence of the novel parvovirus in crocodile feces samples fed on tilapia (75–86%) was apparently higher than that in crocodiles fed with chicken (4%). Further studies revealed that the prevalence of the novel parvovirus in tilapia feces samples collected at four areas in Hainan Province was between 40 and 90%. Four stains of the novel parvovirus were identified in this study based on sequence analyses of NS1 and all the four strains were found in tilapia in contrast only two of them were detected in crocodile feces. The nearly full-length genome sequence of the tilapia parvovirus HMU-HKU-1 was determined and showed less than 45.50 and 40.38% amino acid identity with other members of Parvoviridae in NS1 and VP1 genes, respectively. Phylogenetic analysis based on the complete helicase domain amino acid sequences showed that the tilapia parvovirus HMU-HKU-1 formed a relatively independent branch in the newly proposed genus Chaphamaparvovirus in the subfamily Hamaparvovirinae according to the ICTV’s most recent taxonomic criteria for Parvoviridae classification. Tilapia parvovirus HMU-HKU-1 likely represented a new species within the new genus Chaphamaparvovirus. The identification of tilapia parvovirus HMU-HKU provides further insight into the viral and genetic diversity of parvoviruses and its infections in tilapia populations need to be evaluated in terms of pathogenicity and production losses in tilapia farming.-
dc.languageeng-
dc.publisherFrontiers Research Foundation. The Journal's web site is located at http://www.frontiersin.org/microbiology/-
dc.relation.ispartofFrontiers in Microbiology-
dc.rightsThis Document is Protected by copyright and was first published by Frontiers. All rights reserved. It is reproduced with permission.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectParvoviridae-
dc.subjectnovel virus-
dc.subjectChaphamaparvovirus-
dc.subjectcrocodile-
dc.subjecttilapia-
dc.titleIdentification of a Novel Ichthyic Parvovirus in Marine Species in Hainan Island, China-
dc.typeArticle-
dc.identifier.emailChan, JFW: jfwchan@hku.hk-
dc.identifier.emailYuen, KY: kyyuen@hkucc.hku.hk-
dc.identifier.authorityChan, JFW=rp01736-
dc.identifier.authorityYuen, KY=rp00366-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3389/fmicb.2019.02815-
dc.identifier.pmid31866980-
dc.identifier.pmcidPMC6907010-
dc.identifier.scopuseid_2-s2.0-85077259780-
dc.identifier.hkuros317254-
dc.identifier.volume10-
dc.identifier.spagearticle no. 2815-
dc.identifier.epagearticle no. 2815-
dc.publisher.placeSwitzerland-

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