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Article: Overcoming Multidrug‐Resistant MRSA Using Conventional Aminoglycoside Antibiotics

TitleOvercoming Multidrug‐Resistant MRSA Using Conventional Aminoglycoside Antibiotics
Authors
Keywordsaminoglycoside antibiotics
antibacterial effect
MRSA
photothermal treatment
red phosphorus nanoparticles
Issue Date2020
PublisherWiley Open Access. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)2198-3844
Citation
Advanced Science, 2020, v. 7 n. 9, p. article no. 1902070 How to Cite?
AbstractGlobal multidrug‐resistant (MDR) bacteria are spreading rapidly and causing a great threat to human health due to the abuse of antibiotics. Determining how to resensitize MDR bacteria to conventional inefficient antibiotics is of extreme urgency. Here, a low‐temperature photothermal treatment (PTT, 45 °C) is utilized with red phosphorus nanoparticles to resensitize methicillin‐resistant Staphylococcus aureus (MRSA) to conventional aminoglycoside antibiotics. The antibacterial mechanism is studied by the proteomic technique and molecular dynamics (MD) simulation, which proves that the aminoglycoside antibiotics against MRSA can be selectively potentiated by low‐temperature PTT. The catalytic activity of 2‐aminoglycoside phosphotransferase (APH (2″))—a modifying enzyme—is demonstrated to be obviously inhibited via detecting the consumption of adenosine triphosphate (ATP) in the catalytic reaction. It is also found that the active site of aspartic acid (ASP) residues in APH (2″) is thermally unstable from the results of molecular dynamics simulation. Its catalytic ability is inhibited by preventing the deprotonating procedure for the target OH of gentamycin. The combined therapy also exhibits great biocompatibility and successfully treats MRSA infections in vivo. This low‐temperature PTT strategy has the potential to be an exogenous‐modifying enzyme inhibitor for the treatment of MDR bacterial infection.
Persistent Identifierhttp://hdl.handle.net/10722/289133
ISSN
2018 Impact Factor: 15.804
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorTAN, L-
dc.contributor.authorZHOU, Z-
dc.contributor.authorLIU, X-
dc.contributor.authorLI, J-
dc.contributor.authorZHENG, Y-
dc.contributor.authorCUI, Z-
dc.contributor.authorYANG, X-
dc.contributor.authorLIANG, Y-
dc.contributor.authorLI, Z-
dc.contributor.authorFENG, X-
dc.contributor.authorZHU, S-
dc.contributor.authorYeung, KWK-
dc.contributor.authorYANG, C-
dc.contributor.authorWANG, X-
dc.contributor.authorWU, S-
dc.date.accessioned2020-10-22T08:08:17Z-
dc.date.available2020-10-22T08:08:17Z-
dc.date.issued2020-
dc.identifier.citationAdvanced Science, 2020, v. 7 n. 9, p. article no. 1902070-
dc.identifier.issn2198-3844-
dc.identifier.urihttp://hdl.handle.net/10722/289133-
dc.description.abstractGlobal multidrug‐resistant (MDR) bacteria are spreading rapidly and causing a great threat to human health due to the abuse of antibiotics. Determining how to resensitize MDR bacteria to conventional inefficient antibiotics is of extreme urgency. Here, a low‐temperature photothermal treatment (PTT, 45 °C) is utilized with red phosphorus nanoparticles to resensitize methicillin‐resistant Staphylococcus aureus (MRSA) to conventional aminoglycoside antibiotics. The antibacterial mechanism is studied by the proteomic technique and molecular dynamics (MD) simulation, which proves that the aminoglycoside antibiotics against MRSA can be selectively potentiated by low‐temperature PTT. The catalytic activity of 2‐aminoglycoside phosphotransferase (APH (2″))—a modifying enzyme—is demonstrated to be obviously inhibited via detecting the consumption of adenosine triphosphate (ATP) in the catalytic reaction. It is also found that the active site of aspartic acid (ASP) residues in APH (2″) is thermally unstable from the results of molecular dynamics simulation. Its catalytic ability is inhibited by preventing the deprotonating procedure for the target OH of gentamycin. The combined therapy also exhibits great biocompatibility and successfully treats MRSA infections in vivo. This low‐temperature PTT strategy has the potential to be an exogenous‐modifying enzyme inhibitor for the treatment of MDR bacterial infection.-
dc.languageeng-
dc.publisherWiley Open Access. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)2198-3844-
dc.relation.ispartofAdvanced Science-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectaminoglycoside antibiotics-
dc.subjectantibacterial effect-
dc.subjectMRSA-
dc.subjectphotothermal treatment-
dc.subjectred phosphorus nanoparticles-
dc.titleOvercoming Multidrug‐Resistant MRSA Using Conventional Aminoglycoside Antibiotics-
dc.typeArticle-
dc.identifier.emailYeung, KWK: wkkyeung@hku.hk-
dc.identifier.authorityYeung, KWK=rp00309-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1002/advs.201902070-
dc.identifier.pmid32382474-
dc.identifier.pmcidPMC7201259-
dc.identifier.scopuseid_2-s2.0-85081719746-
dc.identifier.hkuros317540-
dc.identifier.volume7-
dc.identifier.issue9-
dc.identifier.spagearticle no. 1902070-
dc.identifier.epagearticle no. 1902070-
dc.publisher.placeGermany-

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