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Article: Functional characterization of a candidate tumor suppressor gene, Mirror Image Polydactyly 1, in nasopharyngeal carcinoma

TitleFunctional characterization of a candidate tumor suppressor gene, Mirror Image Polydactyly 1, in nasopharyngeal carcinoma
Authors
KeywordsMIPOL1
NPC
RhoB
angiogenesis
metastasis
Issue Date2020
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
Citation
International Journal of Cancer, 2020, v. 146 n. 10, p. 2891-2900 How to Cite?
AbstractMirror Image Polydactyly 1 (MIPOL1) is generally associated with congenital anomalies. However, its role in cancer development is poorly understood. Previously, by utilizing the functional complementation approach, microcell‐mediated chromosome transfer (MMCT), a tumor suppressor gene, MIPOL1, was identified. MIPOL1 was confirmed to be downregulated in nasopharyngeal carcinoma (NPC) cells and tumor tissues, and re‐expression of MIPOL1 induced tumor suppression. The aim of the current study is to further elucidate the functional tumor suppressive role of MIPOL1. In our study, with an expanded sample size of different clinical stages of NPC tumor tissues, we further confirmed the downregulation of MIPOL1 in different cancer stages. MIPOL1 re‐expression down‐regulated angiogenic factors and reduced phosphorylation of metastasis‐associated proteins including AKT, p65, and FAK. In addition, MIPOL1 was confirmed to interact with a tumor suppressor, RhoB, and re‐expression of MIPOL1 enhanced RhoB activity. The functional role of MIPOL1 was further validated by utilizing a panel of wild‐type (WT) and truncated MIPOL1 expression constructs. The MIPOL1 tumor‐suppressive effect can only be observed in the WT MIPOL1‐expressing cells. In vitro and nude mice in vivo functional studies further confirmed the critical role of WT MIPOL1 in inhibiting migration, invasion and metastasis in NPC. Overall, our study provides strong evidence about the tumor‐suppressive role of MIPOL1 in inhibiting angiogenesis and metastasis in NPC.
Persistent Identifierhttp://hdl.handle.net/10722/287640
ISSN
2019 Impact Factor: 5.145
2015 SCImago Journal Rankings: 2.657
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLeong, MML-
dc.contributor.authorCheung, AKL-
dc.contributor.authorKwok, TCT-
dc.contributor.authorLung, ML-
dc.date.accessioned2020-10-05T12:01:04Z-
dc.date.available2020-10-05T12:01:04Z-
dc.date.issued2020-
dc.identifier.citationInternational Journal of Cancer, 2020, v. 146 n. 10, p. 2891-2900-
dc.identifier.issn0020-7136-
dc.identifier.urihttp://hdl.handle.net/10722/287640-
dc.description.abstractMirror Image Polydactyly 1 (MIPOL1) is generally associated with congenital anomalies. However, its role in cancer development is poorly understood. Previously, by utilizing the functional complementation approach, microcell‐mediated chromosome transfer (MMCT), a tumor suppressor gene, MIPOL1, was identified. MIPOL1 was confirmed to be downregulated in nasopharyngeal carcinoma (NPC) cells and tumor tissues, and re‐expression of MIPOL1 induced tumor suppression. The aim of the current study is to further elucidate the functional tumor suppressive role of MIPOL1. In our study, with an expanded sample size of different clinical stages of NPC tumor tissues, we further confirmed the downregulation of MIPOL1 in different cancer stages. MIPOL1 re‐expression down‐regulated angiogenic factors and reduced phosphorylation of metastasis‐associated proteins including AKT, p65, and FAK. In addition, MIPOL1 was confirmed to interact with a tumor suppressor, RhoB, and re‐expression of MIPOL1 enhanced RhoB activity. The functional role of MIPOL1 was further validated by utilizing a panel of wild‐type (WT) and truncated MIPOL1 expression constructs. The MIPOL1 tumor‐suppressive effect can only be observed in the WT MIPOL1‐expressing cells. In vitro and nude mice in vivo functional studies further confirmed the critical role of WT MIPOL1 in inhibiting migration, invasion and metastasis in NPC. Overall, our study provides strong evidence about the tumor‐suppressive role of MIPOL1 in inhibiting angiogenesis and metastasis in NPC.-
dc.languageeng-
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home-
dc.relation.ispartofInternational Journal of Cancer-
dc.rightsThis is the peer reviewed version of the following article: International Journal of Cancer, 2020, v. 146 n. 10, p. 2891-2900, which has been published in final form at https://doi.org/10.1002/ijc.32732. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.-
dc.subjectMIPOL1-
dc.subjectNPC-
dc.subjectRhoB-
dc.subjectangiogenesis-
dc.subjectmetastasis-
dc.titleFunctional characterization of a candidate tumor suppressor gene, Mirror Image Polydactyly 1, in nasopharyngeal carcinoma-
dc.typeArticle-
dc.identifier.emailCheung, AKL: arthurhk@hku.hk-
dc.identifier.emailLung, ML: mlilung@hku.hk-
dc.identifier.authorityCheung, AKL=rp01769-
dc.identifier.authorityLung, ML=rp00300-
dc.description.naturepostprint-
dc.identifier.doi10.1002/ijc.32732-
dc.identifier.pmid31609475-
dc.identifier.scopuseid_2-s2.0-85074825874-
dc.identifier.hkuros314781-
dc.identifier.volume146-
dc.identifier.issue10-
dc.identifier.spage2891-
dc.identifier.epage2900-
dc.identifier.isiWOS:000493579100001-
dc.publisher.placeUnited States-
dc.identifier.issnl0020-7136-

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