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Article: Alternative splicing is a Sorghum bicolor defense response to fungal infection

TitleAlternative splicing is a Sorghum bicolor defense response to fungal infection
Authors
KeywordsMetabolic pathway
Post-transcriptional regulation
Splice site
Splicing factor
Issue Date2020
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00425
Citation
Planta, 2020, v. 251 n. 1, p. article no. 14 How to Cite?
AbstractThis study provides new insights that alternative splicing participates with transcriptional control in defense responses to Colletotrichum sublineola in sorghum In eukaryotic organisms, alternative splicing (AS) is an important post-transcriptional mechanism to generate multiple transcript isoforms from a single gene. Protein variants translated from splicing isoforms may have altered molecular characteristics in signal transduction and metabolic activities. However, which transcript isoforms will be translated into proteins and the biological functions of the resulting proteoforms are yet to be identified. Sorghum is one of the five major cereal crops, but its production is severely affected by fungal diseases. For example, sorghum anthracnose caused by Colletotrichum sublineola greatly reduces grain yield and biomass production. In this study, next-generation sequencing technology was used to analyze C. sublineola-inoculated sorghum seedlings compared with mock-inoculated control. It was identified that AS regulation may be as important as traditional transcriptional control during defense responses to fungal infection. Moreover, several genes involved in flavonoid and phenylpropanoid biosynthetic pathways were found to undergo multiple AS modifications. Further analysis demonstrated that non-conventional targets of both 5'- and 3'-splice sites were alternatively used in response to C. sublineola infection. Splicing factors were also affected at both transcriptional and post-transcriptional levels. As the first transcriptome report on C. sublineola infected sorghum, our work also suggested that AS plays crucial functions in defense responses to fungal invasion.
Persistent Identifierhttp://hdl.handle.net/10722/280931
ISSN
2019 Impact Factor: 3.39
2015 SCImago Journal Rankings: 1.470

 

DC FieldValueLanguage
dc.contributor.authorWANG, L-
dc.contributor.authorChen, M-
dc.contributor.authorZhu, F-
dc.contributor.authorFan, T-
dc.contributor.authorZhang, J-
dc.contributor.authorLo, C-
dc.date.accessioned2020-02-25T07:42:55Z-
dc.date.available2020-02-25T07:42:55Z-
dc.date.issued2020-
dc.identifier.citationPlanta, 2020, v. 251 n. 1, p. article no. 14-
dc.identifier.issn0032-0935-
dc.identifier.urihttp://hdl.handle.net/10722/280931-
dc.description.abstractThis study provides new insights that alternative splicing participates with transcriptional control in defense responses to Colletotrichum sublineola in sorghum In eukaryotic organisms, alternative splicing (AS) is an important post-transcriptional mechanism to generate multiple transcript isoforms from a single gene. Protein variants translated from splicing isoforms may have altered molecular characteristics in signal transduction and metabolic activities. However, which transcript isoforms will be translated into proteins and the biological functions of the resulting proteoforms are yet to be identified. Sorghum is one of the five major cereal crops, but its production is severely affected by fungal diseases. For example, sorghum anthracnose caused by Colletotrichum sublineola greatly reduces grain yield and biomass production. In this study, next-generation sequencing technology was used to analyze C. sublineola-inoculated sorghum seedlings compared with mock-inoculated control. It was identified that AS regulation may be as important as traditional transcriptional control during defense responses to fungal infection. Moreover, several genes involved in flavonoid and phenylpropanoid biosynthetic pathways were found to undergo multiple AS modifications. Further analysis demonstrated that non-conventional targets of both 5'- and 3'-splice sites were alternatively used in response to C. sublineola infection. Splicing factors were also affected at both transcriptional and post-transcriptional levels. As the first transcriptome report on C. sublineola infected sorghum, our work also suggested that AS plays crucial functions in defense responses to fungal invasion.-
dc.languageeng-
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00425-
dc.relation.ispartofPlanta-
dc.rightsThis is a post-peer-review, pre-copyedit version of an article published in [insert journal title]. The final authenticated version is available online at: http://dx.doi.org/[insert DOI]-
dc.subjectMetabolic pathway-
dc.subjectPost-transcriptional regulation-
dc.subjectSplice site-
dc.subjectSplicing factor-
dc.titleAlternative splicing is a Sorghum bicolor defense response to fungal infection-
dc.typeArticle-
dc.identifier.emailLo, C: clivelo@hku.hk-
dc.identifier.authorityLo, C=rp00751-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00425-019-03309-w-
dc.identifier.pmid31776670-
dc.identifier.scopuseid_2-s2.0-85075572835-
dc.identifier.hkuros309222-
dc.identifier.volume251-
dc.identifier.issue1-
dc.identifier.spagearticle no. 14-
dc.identifier.epagearticle no. 14-
dc.publisher.placeGermany-

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