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Article: Sulfonation of Lactobacillus plantarum WLPL04 exopolysaccharide amplifies its antioxidant activities in vitro and in a Caco-2 cell model

TitleSulfonation of Lactobacillus plantarum WLPL04 exopolysaccharide amplifies its antioxidant activities in vitro and in a Caco-2 cell model
Authors
KeywordsLactobacillus plantarum WLPL04
exopolysaccharide
sulfonation
antioxidant activity
Issue Date2019
PublisherAmerican Dairy Science Association. The Journal's web site is located at http://www.journalofdairyscience.org/
Citation
Journal of Dairy Science, 2019, v. 102 n. 7, p. 5922-5932 How to Cite?
AbstractExopolysaccharide (EPS) of Lactobacillus plantarum WLPL04 and its sulfated EPS were systematically investigated for their antioxidant activities and effects on protecting the oxidative damage of Caco-2 cells from H2O2. Exopolysaccharide was successfully sulfonated from purified EPS as confirmed by Fourier-transform infrared spectroscopy, and the degree of sulfonation was 0.30. Both EPS and sulfated EPS showed antioxidant activities in vitro determined by 1,1-diphenyl-2-picrylhydrazyl, superoxide, and hydroxyl radical scavenging tests, and those activities of sulfated EPS were significantly enhanced at 1,000 μg/mL. Cell viabilities of Caco-2 in the range of 1 to 100 μg/mL of EPS and sulfated EPS showed no significant difference. In H2O2-damaged Caco-2 cells models, EPS and sulfated EPS significantly inhibited the enhancement of reactive oxygen species and malondialdehyde levels, and sulfated EPS enhanced the effects by 40.86% and 61.11% when compared with the purified EPS at the same concentration of 100 μg/mL, respectively. For the activities of antioxidant-related enzymes (superoxide dismutase, catalase, and glutathione peroxidase) and expression of genes (SOD2, GPX2, MT1M) on Caco-2 cells, strong protection abilities against the oxidative stress were displayed from both EPS and sulfated EPS, and sulfated EPS exhibited significant enhancement as compared with either EPS or control groups. In summary, sulfonation is an effective strategy for improving the antioxidant activities of EPS from L. plantarum WLPL04 in vitro and on Caco-2 cells.
Persistent Identifierhttp://hdl.handle.net/10722/277594
ISSN
2017 Impact Factor: 2.749
2015 SCImago Journal Rankings: 1.401

 

DC FieldValueLanguage
dc.contributor.authorLiu, Z-
dc.contributor.authorDong, L-
dc.contributor.authorJia, K-
dc.contributor.authorZhan, H-
dc.contributor.authorZhang, Z-
dc.contributor.authorShah, NP-
dc.contributor.authorTao, X-
dc.contributor.authorWei, H-
dc.date.accessioned2019-09-20T08:54:00Z-
dc.date.available2019-09-20T08:54:00Z-
dc.date.issued2019-
dc.identifier.citationJournal of Dairy Science, 2019, v. 102 n. 7, p. 5922-5932-
dc.identifier.issn0022-0302-
dc.identifier.urihttp://hdl.handle.net/10722/277594-
dc.description.abstractExopolysaccharide (EPS) of Lactobacillus plantarum WLPL04 and its sulfated EPS were systematically investigated for their antioxidant activities and effects on protecting the oxidative damage of Caco-2 cells from H2O2. Exopolysaccharide was successfully sulfonated from purified EPS as confirmed by Fourier-transform infrared spectroscopy, and the degree of sulfonation was 0.30. Both EPS and sulfated EPS showed antioxidant activities in vitro determined by 1,1-diphenyl-2-picrylhydrazyl, superoxide, and hydroxyl radical scavenging tests, and those activities of sulfated EPS were significantly enhanced at 1,000 μg/mL. Cell viabilities of Caco-2 in the range of 1 to 100 μg/mL of EPS and sulfated EPS showed no significant difference. In H2O2-damaged Caco-2 cells models, EPS and sulfated EPS significantly inhibited the enhancement of reactive oxygen species and malondialdehyde levels, and sulfated EPS enhanced the effects by 40.86% and 61.11% when compared with the purified EPS at the same concentration of 100 μg/mL, respectively. For the activities of antioxidant-related enzymes (superoxide dismutase, catalase, and glutathione peroxidase) and expression of genes (SOD2, GPX2, MT1M) on Caco-2 cells, strong protection abilities against the oxidative stress were displayed from both EPS and sulfated EPS, and sulfated EPS exhibited significant enhancement as compared with either EPS or control groups. In summary, sulfonation is an effective strategy for improving the antioxidant activities of EPS from L. plantarum WLPL04 in vitro and on Caco-2 cells.-
dc.languageeng-
dc.publisherAmerican Dairy Science Association. The Journal's web site is located at http://www.journalofdairyscience.org/-
dc.relation.ispartofJournal of Dairy Science-
dc.subjectLactobacillus plantarum WLPL04-
dc.subjectexopolysaccharide-
dc.subjectsulfonation-
dc.subjectantioxidant activity-
dc.titleSulfonation of Lactobacillus plantarum WLPL04 exopolysaccharide amplifies its antioxidant activities in vitro and in a Caco-2 cell model-
dc.typeArticle-
dc.identifier.emailShah, NP: npshah@hku.hk-
dc.identifier.authorityShah, NP=rp01571-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.3168/jds.2018-15831-
dc.identifier.pmid31030915-
dc.identifier.scopuseid_2-s2.0-85064669589-
dc.identifier.hkuros305584-
dc.identifier.volume102-
dc.identifier.issue7-
dc.identifier.spage5922-
dc.identifier.epage5932-
dc.publisher.placeUnited States-

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