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Article: Connexin 43 is involved in early differentiation of human embryonic stem cells

TitleConnexin 43 is involved in early differentiation of human embryonic stem cells
Authors
KeywordsHESC
Connexin 43
GJIC
Differentiation
Issue Date2018
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/717204/description#description
Citation
Differentiation, 2018, v. 105, p. 33-44 How to Cite?
AbstractGap junctional intercellular communication (GJIC) is important for maintaining the pluripotency of mouse embryonic stem cells (mESC). However, human ESC (hESC) have a high level of connexin (Cx) molecules with unknown function. In this study, we found that the major Cx molecule, Cx43, was highly expressed in undifferentiated hESC. It was down-regulated upon spontaneously differentiation by embryoid body formation and induced differentiation along ectoderm, mesoderm and extraembryonic lineages, but up-regulated along endoderm differentiation. The knockdown of Cx43 and GJIC had no effect on the maintenance of hESC, as demonstrated by no morphological changes and similar expression levels of pluripotent markers (OCT4, NANOG, SSEA-3 and SSEA-4) and early differentiation markers (KRT8 and KRT18). Meanwhile, Cx43 knock down had no effect on endodermal markers (SOX17, FOXA2 and CXCR4) expression when hESC were differentiating into definitive endoderm lineage. On the contrary, it led to lower levels of mesodermal markers (CD56, CD34 and PDGFR-α) when cells were undergoing mesoderm differentiation. When compared to control, Cx43 knockdown led to higher attachment rate, HCG secretion and cell invasion of the hESC derived trophoblastic cells. Cx43 knockdown also resulted in up-regulated expressions of placental hormone (β-hCG) and implantation related genes (LIFR, CDH5, LEP, PGF, TGFBR2). Our study suggested that Cx43 and GJIC had no effect on the undifferentiated growth of hESC but affected specific lineage differentiation.
Persistent Identifierhttp://hdl.handle.net/10722/277258
ISSN
2017 Impact Factor: 2.688
2015 SCImago Journal Rankings: 1.747

 

DC FieldValueLanguage
dc.contributor.authorPeng, Q-
dc.contributor.authorYUE, C-
dc.contributor.authorChen, ACH-
dc.contributor.authorLee, KC-
dc.contributor.authorFong, SW-
dc.contributor.authorYeung, WSB-
dc.contributor.authorLee, YL-
dc.date.accessioned2019-09-20T08:47:37Z-
dc.date.available2019-09-20T08:47:37Z-
dc.date.issued2018-
dc.identifier.citationDifferentiation, 2018, v. 105, p. 33-44-
dc.identifier.issn0301-4681-
dc.identifier.urihttp://hdl.handle.net/10722/277258-
dc.description.abstractGap junctional intercellular communication (GJIC) is important for maintaining the pluripotency of mouse embryonic stem cells (mESC). However, human ESC (hESC) have a high level of connexin (Cx) molecules with unknown function. In this study, we found that the major Cx molecule, Cx43, was highly expressed in undifferentiated hESC. It was down-regulated upon spontaneously differentiation by embryoid body formation and induced differentiation along ectoderm, mesoderm and extraembryonic lineages, but up-regulated along endoderm differentiation. The knockdown of Cx43 and GJIC had no effect on the maintenance of hESC, as demonstrated by no morphological changes and similar expression levels of pluripotent markers (OCT4, NANOG, SSEA-3 and SSEA-4) and early differentiation markers (KRT8 and KRT18). Meanwhile, Cx43 knock down had no effect on endodermal markers (SOX17, FOXA2 and CXCR4) expression when hESC were differentiating into definitive endoderm lineage. On the contrary, it led to lower levels of mesodermal markers (CD56, CD34 and PDGFR-α) when cells were undergoing mesoderm differentiation. When compared to control, Cx43 knockdown led to higher attachment rate, HCG secretion and cell invasion of the hESC derived trophoblastic cells. Cx43 knockdown also resulted in up-regulated expressions of placental hormone (β-hCG) and implantation related genes (LIFR, CDH5, LEP, PGF, TGFBR2). Our study suggested that Cx43 and GJIC had no effect on the undifferentiated growth of hESC but affected specific lineage differentiation.-
dc.languageeng-
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/717204/description#description-
dc.relation.ispartofDifferentiation-
dc.subjectHESC-
dc.subjectConnexin 43-
dc.subjectGJIC-
dc.subjectDifferentiation-
dc.titleConnexin 43 is involved in early differentiation of human embryonic stem cells-
dc.typeArticle-
dc.identifier.emailChen, ACH: andycch0@hku.hk-
dc.identifier.emailLee, KC: chuenlee@hku.hk-
dc.identifier.emailFong, SW: szewan11@hku.hk-
dc.identifier.emailYeung, WSB: wsbyeung@hku.hk-
dc.identifier.emailLee, YL: cherielee@hku.hk-
dc.identifier.authorityYeung, WSB=rp00331-
dc.identifier.authorityLee, YL=rp00308-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.diff.2018.12.003-
dc.identifier.pmid30599359-
dc.identifier.scopuseid_2-s2.0-85059177619-
dc.identifier.hkuros305914-
dc.identifier.volume105-
dc.identifier.spage33-
dc.identifier.epage44-
dc.publisher.placeUnited Kingdom-

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