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Article: Interaction and fusion dynamics between cellular blebs

TitleInteraction and fusion dynamics between cellular blebs
Authors
KeywordsCell adhesion
Cell blebbing
Membrane deformation
Issue Date2018
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/jbiomech
Citation
Journal of Biomechanics, 2018, v. 81, p. 113-121 How to Cite?
AbstractMembrane blebbing, as a mechanism for cells to regulate their internal pressure and membrane tension, is believed to play important roles in processes such as cell migration, spreading and apoptosis. However, the fundamental question of how different blebs interact with each other during their life cycles remains largely unclear. Here, we report a combined theoretical and experimental investigation to examine how the growth and retraction of a cellular bleb are influenced by neighboring blebs as well as the fusion dynamics between them. Specifically, a boundary integral model was developed to describe the shape evolution of cell membrane during the blebbing/retracting process. We showed that a drop in the intracellular pressure will be induced by the formation of a bleb whose retraction then restores the pressure level. Consequently, the volume that a second bleb can reach was predicted to heavily depend on its initial weakened size and the time lag with respect to the first bleb, all in quantitative agreement with our experimental observations. In addition, it was found that as the strength of membrane-cortex adhesion increases, the possible coalescence of two neighboring blebs changes from smooth fusion to abrupt coalescence and eventually to no fusion at all. Phase diagrams summarizing the dependence of such transition on key physical factors, such as the intracellular pressure and bleb separation, were also obtained. © 2018 Elsevier Ltd
Persistent Identifierhttp://hdl.handle.net/10722/275059
ISSN
2017 Impact Factor: 2.431
2015 SCImago Journal Rankings: 1.233
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorFang, C-
dc.contributor.authorHui, TH-
dc.contributor.authorWwi, X-
dc.contributor.authorYan, Z-
dc.contributor.authorQian, J-
dc.contributor.authorLin, Y-
dc.date.accessioned2019-09-10T02:34:36Z-
dc.date.available2019-09-10T02:34:36Z-
dc.date.issued2018-
dc.identifier.citationJournal of Biomechanics, 2018, v. 81, p. 113-121-
dc.identifier.issn0021-9290-
dc.identifier.urihttp://hdl.handle.net/10722/275059-
dc.description.abstractMembrane blebbing, as a mechanism for cells to regulate their internal pressure and membrane tension, is believed to play important roles in processes such as cell migration, spreading and apoptosis. However, the fundamental question of how different blebs interact with each other during their life cycles remains largely unclear. Here, we report a combined theoretical and experimental investigation to examine how the growth and retraction of a cellular bleb are influenced by neighboring blebs as well as the fusion dynamics between them. Specifically, a boundary integral model was developed to describe the shape evolution of cell membrane during the blebbing/retracting process. We showed that a drop in the intracellular pressure will be induced by the formation of a bleb whose retraction then restores the pressure level. Consequently, the volume that a second bleb can reach was predicted to heavily depend on its initial weakened size and the time lag with respect to the first bleb, all in quantitative agreement with our experimental observations. In addition, it was found that as the strength of membrane-cortex adhesion increases, the possible coalescence of two neighboring blebs changes from smooth fusion to abrupt coalescence and eventually to no fusion at all. Phase diagrams summarizing the dependence of such transition on key physical factors, such as the intracellular pressure and bleb separation, were also obtained. © 2018 Elsevier Ltd-
dc.languageeng-
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/jbiomech-
dc.relation.ispartofJournal of Biomechanics-
dc.subjectCell adhesion-
dc.subjectCell blebbing-
dc.subjectMembrane deformation-
dc.titleInteraction and fusion dynamics between cellular blebs-
dc.typeArticle-
dc.identifier.emailLin, Y: ylin@hkucc.hku.hk-
dc.identifier.authorityLin, Y=rp00080-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jbiomech.2018.10.004-
dc.identifier.pmid30366658-
dc.identifier.scopuseid_2-s2.0-85055172723-
dc.identifier.hkuros304182-
dc.identifier.volume81-
dc.identifier.spage113-
dc.identifier.epage121-
dc.identifier.isiWOS:000451104300013-
dc.publisher.placeUnited Kingdom-

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