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Conference Paper: High-throughput screening of therapeutic combinations against ovarian cancer

TitleHigh-throughput screening of therapeutic combinations against ovarian cancer
Authors
Issue Date2019
PublisherAmerican Association for Cancer Research.
Citation
American Association for Cancer Research (AACR) Annual Meeting 2019, Atlanta, USA, 29 March - 3 April 2019 How to Cite?
AbstractApplying synergistic drug combinations can increase efficacy of cancer treatment. However, conventional methods for identifying those combinations involve testing of every possible combination including different dosages for each agent, are difficult to scale due to the huge amount of efforts and costs needed. To tackle this problem, we have developed CombiGEM-CRISPR (combinatorial genetics en masse-CRISPR) to enable high-throughput assembly and screening of pair-wise gene combinations. By applying CombiGEM-CRISPR,phenotypes of combinatorial gene knockouts could be used to predict the effects of drug inhibitor combinations targeting the matching gene products. We designed a pair-wise sgRNA library comprising 25,281 combinations for interrogating 52 genes that are overexpressed in ovarian cancers. By pooled evaluation of their effects on cell proliferation, our screening identified new combinations that inhibit ovarian cancer cell growth. Therefore, our work accelerates the discovery of new therapeutic options against ovarian cancer through applying CombiGEM-CRISPR.
DescriptionSession PO.BSB02.01 - Convergence Science for Therapeutics and Precision Medicine
Persistent Identifierhttp://hdl.handle.net/10722/274599

 

DC FieldValueLanguage
dc.contributor.authorZhou, P-
dc.contributor.authorWong, SL-
dc.date.accessioned2019-08-18T15:05:02Z-
dc.date.available2019-08-18T15:05:02Z-
dc.date.issued2019-
dc.identifier.citationAmerican Association for Cancer Research (AACR) Annual Meeting 2019, Atlanta, USA, 29 March - 3 April 2019-
dc.identifier.urihttp://hdl.handle.net/10722/274599-
dc.descriptionSession PO.BSB02.01 - Convergence Science for Therapeutics and Precision Medicine-
dc.description.abstractApplying synergistic drug combinations can increase efficacy of cancer treatment. However, conventional methods for identifying those combinations involve testing of every possible combination including different dosages for each agent, are difficult to scale due to the huge amount of efforts and costs needed. To tackle this problem, we have developed CombiGEM-CRISPR (combinatorial genetics en masse-CRISPR) to enable high-throughput assembly and screening of pair-wise gene combinations. By applying CombiGEM-CRISPR,phenotypes of combinatorial gene knockouts could be used to predict the effects of drug inhibitor combinations targeting the matching gene products. We designed a pair-wise sgRNA library comprising 25,281 combinations for interrogating 52 genes that are overexpressed in ovarian cancers. By pooled evaluation of their effects on cell proliferation, our screening identified new combinations that inhibit ovarian cancer cell growth. Therefore, our work accelerates the discovery of new therapeutic options against ovarian cancer through applying CombiGEM-CRISPR.-
dc.languageeng-
dc.publisherAmerican Association for Cancer Research. -
dc.relation.ispartofAmerican Association for Cancer Research (AACR) Annual Meeting-
dc.titleHigh-throughput screening of therapeutic combinations against ovarian cancer -
dc.typeConference_Paper-
dc.identifier.emailZhou, P: zhoupeng@hku.hk-
dc.identifier.emailWong, SL: aslw@hku.hk-
dc.identifier.authorityWong, SL=rp02139-
dc.identifier.hkuros301306-
dc.publisher.placeAtlanta, USA-

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