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Article: Crucifera sulforaphane (SFN) inhibits the growth of nasopharyngeal carcinoma through DNA methyltransferase 1 (DNMT1)/Wnt inhibitory factor 1 (WIF1) axis.

TitleCrucifera sulforaphane (SFN) inhibits the growth of nasopharyngeal carcinoma through DNA methyltransferase 1 (DNMT1)/Wnt inhibitory factor 1 (WIF1) axis.
Authors
KeywordsSulforaphane
Nasopharyngeal carcinoma
DNMT1
WIF1
Issue Date2019
PublisherElsevier GmbH - Urban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/phytomed
Citation
Phytomedicine, 2019, v. 63, article no. 153058 How to Cite?
AbstractBackground Sulforaphane (SFN), a natural compound present in cruciferous vegetable, has been shown to possess anti-cancer activities. Cancer stem cell (CSC) in bulk tumor is generally considered as treatment resistant cell and involved in cancer recurrence. The effects of SFN on nasopharyngeal carcinoma (NPC) CSCs have not yet been explored. Purpose The present study aims to examine the anti-tumor activities of SFN on NPC cells with CSC-like properties and the underlying mechanisms. Methods NPC cells growing in monolayer culture, CSCs-enriched NPC tumor spheres, and also the NPC nude mice xenograft were used to study the anti-tumor activities of SFN on NPC. The population of cells expressing CSC-associated markers was evaluated using flow cytometry and aldehyde dehydrogenase (ALDH) activity assay. The effect of DNA methyltransferase 1 (DNMT1) on the growth of NPC cells was analyzed by using small interfering RNA (siRNA)-mediated silencing method. Results SFN was found to inhibit the formation of CSC-enriched NPC tumor spheres and reduce the population of cells with CSC-associated properties (SRY (Sex determining Region Y)-box 2 (SOX2) and ALDH). In the functional study, SFN was found to restore the expression of Wnt inhibitory factor 1 (WIF1) and the effect was accompanied with the downregulation of DNMT1. The functional activities of WIF1 and DNMT1 were confirmed using exogenously added recombinant WIF1 and siRNA knockdown of DNMT1. Moreover, SFN was found to inhibit the in vivo growth of C666-1 cells and enhance the anti-tumor effects of cisplatin. Conclusion Taken together, we demonstrated that SFN could suppress the growth of NPC cells via the DNMT1/WIF1 axis.
Persistent Identifierhttp://hdl.handle.net/10722/273854
ISSN
2019 Impact Factor: 4.268
2015 SCImago Journal Rankings: 1.013
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChen, L-
dc.contributor.authorChan, LS-
dc.contributor.authorLung, HL-
dc.contributor.authorYip, TTC-
dc.contributor.authorNgan, KCR-
dc.contributor.authorWong, JWC-
dc.contributor.authorLo, KW-
dc.contributor.authorNg, WT-
dc.contributor.authorLee, WMA-
dc.contributor.authorTsao, GSW-
dc.contributor.authorLung, ML-
dc.contributor.authorMak, NK-
dc.date.accessioned2019-08-18T14:49:56Z-
dc.date.available2019-08-18T14:49:56Z-
dc.date.issued2019-
dc.identifier.citationPhytomedicine, 2019, v. 63, article no. 153058-
dc.identifier.issn0944-7113-
dc.identifier.urihttp://hdl.handle.net/10722/273854-
dc.description.abstractBackground Sulforaphane (SFN), a natural compound present in cruciferous vegetable, has been shown to possess anti-cancer activities. Cancer stem cell (CSC) in bulk tumor is generally considered as treatment resistant cell and involved in cancer recurrence. The effects of SFN on nasopharyngeal carcinoma (NPC) CSCs have not yet been explored. Purpose The present study aims to examine the anti-tumor activities of SFN on NPC cells with CSC-like properties and the underlying mechanisms. Methods NPC cells growing in monolayer culture, CSCs-enriched NPC tumor spheres, and also the NPC nude mice xenograft were used to study the anti-tumor activities of SFN on NPC. The population of cells expressing CSC-associated markers was evaluated using flow cytometry and aldehyde dehydrogenase (ALDH) activity assay. The effect of DNA methyltransferase 1 (DNMT1) on the growth of NPC cells was analyzed by using small interfering RNA (siRNA)-mediated silencing method. Results SFN was found to inhibit the formation of CSC-enriched NPC tumor spheres and reduce the population of cells with CSC-associated properties (SRY (Sex determining Region Y)-box 2 (SOX2) and ALDH). In the functional study, SFN was found to restore the expression of Wnt inhibitory factor 1 (WIF1) and the effect was accompanied with the downregulation of DNMT1. The functional activities of WIF1 and DNMT1 were confirmed using exogenously added recombinant WIF1 and siRNA knockdown of DNMT1. Moreover, SFN was found to inhibit the in vivo growth of C666-1 cells and enhance the anti-tumor effects of cisplatin. Conclusion Taken together, we demonstrated that SFN could suppress the growth of NPC cells via the DNMT1/WIF1 axis.-
dc.languageeng-
dc.publisherElsevier GmbH - Urban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/phytomed-
dc.relation.ispartofPhytomedicine-
dc.subjectSulforaphane-
dc.subjectNasopharyngeal carcinoma-
dc.subjectDNMT1-
dc.subjectWIF1-
dc.titleCrucifera sulforaphane (SFN) inhibits the growth of nasopharyngeal carcinoma through DNA methyltransferase 1 (DNMT1)/Wnt inhibitory factor 1 (WIF1) axis.-
dc.typeArticle-
dc.identifier.emailLung, HL: hllung2@hku.hk-
dc.identifier.emailNgan, KCR: rkcngan@hku.hk-
dc.identifier.emailNg, WT: ngwt1@hkucc.hku.hk-
dc.identifier.emailLee, WMA: awmlee@hkucc.hku.hk-
dc.identifier.emailTsao, GSW: gswtsao@hku.hk-
dc.identifier.emailLung, ML: mlilung@hku.hk-
dc.identifier.authorityLung, HL=rp00299-
dc.identifier.authorityNgan, KCR=rp02371-
dc.identifier.authorityLee, WMA=rp02056-
dc.identifier.authorityTsao, GSW=rp00399-
dc.identifier.authorityLung, ML=rp00300-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.phymed.2019.153058-
dc.identifier.pmid31394414-
dc.identifier.scopuseid_2-s2.0-85073705090-
dc.identifier.hkuros301620-
dc.identifier.volume63-
dc.identifier.spagearticle no. 153058-
dc.identifier.epagearticle no. 153058-
dc.identifier.isiWOS:000499699300030-
dc.publisher.placeGermany-

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