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Article: Adhesive and oxidative response of stem cell and pre-osteoblasts on titanium and zirconia surfaces in vitro

TitleAdhesive and oxidative response of stem cell and pre-osteoblasts on titanium and zirconia surfaces in vitro
Authors
KeywordsCell adhesion
Dental implant
Reactive oxygen species
Titanium
Zirconia
Issue Date2019
PublisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=2041-1618&site=1
Citation
Journal of Investigative and Clinical Dentistry, 2019, v. 10 n. 3, article no. e12407 How to Cite?
AbstractAim: The aim of the present study was to investigate the initial stem cell and pre‐osteoblast cell adhesion and oxidative response on zirconia in comparison with titanium. Methods: Human dental pulp stem cells (DPSC) and murine pre‐osteoblasts (MC3T3‐E1) cells were cultured on zirconia and titanium surfaces, and at 3‐, 12‐, and 24‐hour intervals, cell viability and morphology were determined with tetrazolium based colorimetric assay, scanning electron microscopy, and immunofluorescence analysis. The in situ reactive oxygen species level of both cells on each material surface was examined after 24‐hour culture. Results: Both DPSC and MC3T3‐E1 cells revealed comparable morphological features during 24‐hour cell adhesion processes, with cells continued expanding of cell size and increasing of cell viability on titanium and zirconia surfaces during 24‐hour culture. Zirconia demonstrated relatively higher mean cell viability compared to titanium within 24‐hour culture, with significantly higher DPSC viability at 12 hours after seeding (P < 0.05). Relatively higher mean reactive oxygen species levels in both DPSC and MC3T3E1 were found on zirconia surfaces after 24‐hour culture compared to titanium. Conclusions: From the results, zirconia as a potential dental implant substrate demonstrated equivalent or better initial cellular responses compared to titanium.
Persistent Identifierhttp://hdl.handle.net/10722/272128
ISSN
2015 SCImago Journal Rankings: 0.268
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWei, C-
dc.contributor.authorGong, T-
dc.contributor.authorPow, EHN-
dc.contributor.authorBotelho, MG-
dc.date.accessioned2019-07-20T10:36:13Z-
dc.date.available2019-07-20T10:36:13Z-
dc.date.issued2019-
dc.identifier.citationJournal of Investigative and Clinical Dentistry, 2019, v. 10 n. 3, article no. e12407-
dc.identifier.issn2041-1618-
dc.identifier.urihttp://hdl.handle.net/10722/272128-
dc.description.abstractAim: The aim of the present study was to investigate the initial stem cell and pre‐osteoblast cell adhesion and oxidative response on zirconia in comparison with titanium. Methods: Human dental pulp stem cells (DPSC) and murine pre‐osteoblasts (MC3T3‐E1) cells were cultured on zirconia and titanium surfaces, and at 3‐, 12‐, and 24‐hour intervals, cell viability and morphology were determined with tetrazolium based colorimetric assay, scanning electron microscopy, and immunofluorescence analysis. The in situ reactive oxygen species level of both cells on each material surface was examined after 24‐hour culture. Results: Both DPSC and MC3T3‐E1 cells revealed comparable morphological features during 24‐hour cell adhesion processes, with cells continued expanding of cell size and increasing of cell viability on titanium and zirconia surfaces during 24‐hour culture. Zirconia demonstrated relatively higher mean cell viability compared to titanium within 24‐hour culture, with significantly higher DPSC viability at 12 hours after seeding (P < 0.05). Relatively higher mean reactive oxygen species levels in both DPSC and MC3T3E1 were found on zirconia surfaces after 24‐hour culture compared to titanium. Conclusions: From the results, zirconia as a potential dental implant substrate demonstrated equivalent or better initial cellular responses compared to titanium.-
dc.languageeng-
dc.publisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=2041-1618&site=1-
dc.relation.ispartofJournal of Investigative and Clinical Dentistry-
dc.subjectCell adhesion-
dc.subjectDental implant-
dc.subjectReactive oxygen species-
dc.subjectTitanium-
dc.subjectZirconia-
dc.titleAdhesive and oxidative response of stem cell and pre-osteoblasts on titanium and zirconia surfaces in vitro-
dc.typeArticle-
dc.identifier.emailWei, C: cxwei@hku.hk-
dc.identifier.emailPow, EHN: ehnpow@hku.hk-
dc.identifier.emailBotelho, MG: botelho@hkucc.hku.hk-
dc.identifier.authorityPow, EHN=rp00030-
dc.identifier.authorityBotelho, MG=rp00033-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/jicd.12407-
dc.identifier.pmid30866178-
dc.identifier.scopuseid_2-s2.0-85070973231-
dc.identifier.hkuros299067-
dc.identifier.volume10-
dc.identifier.issue3-
dc.identifier.spagearticle no. e12407-
dc.identifier.epagearticle no. e12407-
dc.identifier.isiWOS:000478603700009-
dc.publisher.placeUnited States-

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