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Article: Application of UPLC‐MS/MS to simultaneously detect four bioactive compounds in the tumour‐shrinking decoction (FM1523) for uterine fibroids treatment

TitleApplication of UPLC‐MS/MS to simultaneously detect four bioactive compounds in the tumour‐shrinking decoction (FM1523) for uterine fibroids treatment
Authors
Cheng, ICCLI, RKLeung, GPHLi, SLKong, MLao, LXZhang, ZJLin, WLNg, EHYRong, JHChen, JPSu, JZhang, KYBMeng, W
Keywordsbioactive compounds
extraction
multiple reaction monitoring
tumor‐shrinking decoction
ultra‐pressure liquid chromatography coupled tandem mass spectrometry
Issue Date2019
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/5152
Citation
Phytochemical Analysis, 2019, v. 30 n. 4, p. 447-455 How to Cite?
DOI: http://dx.doi.org/10.1002/pca.2827
AbstractIntroduction: The Chinese medicine formulation, tumour‐shrinking decoction (TSD, FM1523), which consists of 15 natural medicines, is used for uterine fibroids (UFs) therapy and possesses excellent clinical therapeutic effect. Objective: To develop a sensitive and validated analytical method for the simultaneous quantification of four crucial bioactive compounds including isorhamnetin‐3‐O‐neohesperidoside, curcumin, peimine and tetrahydropalmatine in the principal formulation of this decoction. Methods: An ultra‐performance liquid chromatography coupled tandem mass spectrometry (UPLC‐MS/MS) with an electrospray ionisation (ESI) source in multiple reaction monitoring (MRM) mode was conducted to investigate these bioactive compounds in the TSD. The chromatographic separation was performed on a C18 column when the flow rate was adjusted at 0.2 mL/min with gradient elution of acetonitrile–water with 0.1% formic acid. Accelerated solvent extraction (ASE) method with higher extraction efficiency was employed for TSD sample pre‐treatment. Results: The linearity, limit of detection (LOD) and limit of quantification (LOQ) were determined for this analytical method. The mean recoveries of the compounds were determined between 100.23% and 104.02% with satisfactory relative standard deviation (RSD) in the ranges of 2.65% to 3.81%. The precision was evaluated by intra‐day and inter‐day tests, which revealed RSD within the ranges of 1.21% to 2.14% and 1.24% to 2.32%, respectively. Conclusion: The bioactive compounds of TSD samples were successfully quantified via UPLC‐MS/MS with MRM mode. This study could help to evaluate the pharmacokinetic study of TSD during clinical applications and present a facile strategy for quantifying bioactive compounds in traditional Chinese Medicine decoction.
Persistent Identifierhttp://hdl.handle.net/10722/271260
ISSN
0958-0344
2021 Impact Factor: 3.024
2020 SCImago Journal Rankings: 0.574
ISI Accession Number ID
WOS:000469994300008

 

DC FieldValueLanguage
dc.contributor.authorCheng, ICC-
dc.contributor.authorLI, RK-
dc.contributor.authorLeung, GPH-
dc.contributor.authorLi, SL-
dc.contributor.authorKong, M-
dc.contributor.authorLao, LX-
dc.contributor.authorZhang, ZJ-
dc.contributor.authorLin, WL-
dc.contributor.authorNg, EHY-
dc.contributor.authorRong, JH-
dc.contributor.authorChen, JP-
dc.contributor.authorSu, J-
dc.contributor.authorZhang, KYB-
dc.contributor.authorMeng, W-
dc.date.accessioned2019-06-24T01:06:27Z-
dc.date.available2019-06-24T01:06:27Z-
dc.date.issued2019-
dc.identifier.citationPhytochemical Analysis, 2019, v. 30 n. 4, p. 447-455-
dc.identifier.issn0958-0344-
dc.identifier.urihttp://hdl.handle.net/10722/271260-
dc.description.abstractIntroduction: The Chinese medicine formulation, tumour‐shrinking decoction (TSD, FM1523), which consists of 15 natural medicines, is used for uterine fibroids (UFs) therapy and possesses excellent clinical therapeutic effect. Objective: To develop a sensitive and validated analytical method for the simultaneous quantification of four crucial bioactive compounds including isorhamnetin‐3‐O‐neohesperidoside, curcumin, peimine and tetrahydropalmatine in the principal formulation of this decoction. Methods: An ultra‐performance liquid chromatography coupled tandem mass spectrometry (UPLC‐MS/MS) with an electrospray ionisation (ESI) source in multiple reaction monitoring (MRM) mode was conducted to investigate these bioactive compounds in the TSD. The chromatographic separation was performed on a C18 column when the flow rate was adjusted at 0.2 mL/min with gradient elution of acetonitrile–water with 0.1% formic acid. Accelerated solvent extraction (ASE) method with higher extraction efficiency was employed for TSD sample pre‐treatment. Results: The linearity, limit of detection (LOD) and limit of quantification (LOQ) were determined for this analytical method. The mean recoveries of the compounds were determined between 100.23% and 104.02% with satisfactory relative standard deviation (RSD) in the ranges of 2.65% to 3.81%. The precision was evaluated by intra‐day and inter‐day tests, which revealed RSD within the ranges of 1.21% to 2.14% and 1.24% to 2.32%, respectively. Conclusion: The bioactive compounds of TSD samples were successfully quantified via UPLC‐MS/MS with MRM mode. This study could help to evaluate the pharmacokinetic study of TSD during clinical applications and present a facile strategy for quantifying bioactive compounds in traditional Chinese Medicine decoction.-
dc.languageeng-
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/5152-
dc.relation.ispartofPhytochemical Analysis-
dc.rightsThis is the peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.-
dc.subjectbioactive compounds-
dc.subjectextraction-
dc.subjectmultiple reaction monitoring-
dc.subjecttumor‐shrinking decoction-
dc.subjectultra‐pressure liquid chromatography coupled tandem mass spectrometry-
dc.titleApplication of UPLC‐MS/MS to simultaneously detect four bioactive compounds in the tumour‐shrinking decoction (FM1523) for uterine fibroids treatment-
dc.typeArticle-
dc.identifier.emailLeung, GPH: gphleung@hkucc.hku.hk-
dc.identifier.emailLao, LX: lxlao1@hku.hk-
dc.identifier.emailZhang, ZJ: zhangzj@hkucc.hku.hk-
dc.identifier.emailNg, EHY: nghye@hku.hk-
dc.identifier.emailRong, JH: jrong@hku.hk-
dc.identifier.emailChen, JP: abchen@hkucc.hku.hk-
dc.identifier.emailSu, J: sujing59@hku.hk-
dc.identifier.emailZhang, KYB: ybzhang@hku.hk-
dc.identifier.emailMeng, W: bmeng@hku.hk-
dc.identifier.authorityLeung, GPH=rp00234-
dc.identifier.authorityLao, LX=rp01784-
dc.identifier.authorityZhang, ZJ=rp01297-
dc.identifier.authorityNg, EHY=rp00426-
dc.identifier.authorityRong, JH=rp00515-
dc.identifier.authorityChen, JP=rp01316-
dc.identifier.authorityZhang, KYB=rp01410-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/pca.2827-
dc.identifier.pmid30916852-
dc.identifier.scopuseid_2-s2.0-85063549539-
dc.identifier.hkuros297958-
dc.identifier.volume30-
dc.identifier.issue4-
dc.identifier.spage447-
dc.identifier.epage455-
dc.identifier.isiWOS:000469994300008-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl0958-0344-

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