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Article: 3D-Bioprinted Osteoblast-Laden Nanocomposite Hydrogel Constructs with Induced Microenvironments Promote Cell Viability, Differentiation, and Osteogenesis both In Vitro and In Vivo

Title3D-Bioprinted Osteoblast-Laden Nanocomposite Hydrogel Constructs with Induced Microenvironments Promote Cell Viability, Differentiation, and Osteogenesis both In Vitro and In Vivo
Authors
Keywords3D-bioprinting
Nanocomposite hydrogels
Osteoblast-laden constructs
Osteogenesis capability
Issue Date2018
PublisherWiley Open Access. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)2198-3844
Citation
Advanced Science, 2018, v. 5 n. 3, article no. 1700550, p. 1-11 How to Cite?
AbstractAn osteoblast-laden nanocomposite hydrogel construct, based on polyethylene glycol diacrylate (PEGDA)/laponite XLG nanoclay ([Mg5.34Li0.66Si8O20(OH)4]Na0.66, clay)/hyaluronic acid sodium salt (HA) bio-inks, is developed by a two-channel 3D bioprinting method. The novel biodegradable bio-ink A, comprised of a poly(ethylene glycol) (PEG)–clay nanocomposite crosslinked hydrogel, is used to facilitate 3D-bioprinting and enables the efficient delivery of oxygen and nutrients to growing cells. HA with encapsulated primary rat osteoblasts (ROBs) is applied as bio-ink B with a view to improving cell viability, distribution uniformity, and deposition efficiency. The cell-laden PEG–clay constructs not only encapsulated osteoblasts with more than 95% viability in the short term but also exhibited excellent osteogenic ability in the long term, due to the release of bioactive ions (magnesium ions, Mg2+ and silicon ions, Si4+), which induces the suitable microenvironment to promote the differentiation of the loaded exogenous ROBs, both in vitro and in vivo. This 3D-bioprinting method holds much promise for bone tissue regeneration in terms of cell engraftment, survival, and ultimately long-term function.
Persistent Identifierhttp://hdl.handle.net/10722/263388
ISSN
2017 Impact Factor: 12.441
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhai, X-
dc.contributor.authorRuan, C-
dc.contributor.authorMa, Y-
dc.contributor.authorCheng, D-
dc.contributor.authorWu, M-
dc.contributor.authorLiu, W-
dc.contributor.authorZhao, X-
dc.contributor.authorPan, H-
dc.contributor.authorLu, WW-
dc.date.accessioned2018-10-22T07:38:07Z-
dc.date.available2018-10-22T07:38:07Z-
dc.date.issued2018-
dc.identifier.citationAdvanced Science, 2018, v. 5 n. 3, article no. 1700550, p. 1-11-
dc.identifier.issn2198-3844-
dc.identifier.urihttp://hdl.handle.net/10722/263388-
dc.description.abstractAn osteoblast-laden nanocomposite hydrogel construct, based on polyethylene glycol diacrylate (PEGDA)/laponite XLG nanoclay ([Mg5.34Li0.66Si8O20(OH)4]Na0.66, clay)/hyaluronic acid sodium salt (HA) bio-inks, is developed by a two-channel 3D bioprinting method. The novel biodegradable bio-ink A, comprised of a poly(ethylene glycol) (PEG)–clay nanocomposite crosslinked hydrogel, is used to facilitate 3D-bioprinting and enables the efficient delivery of oxygen and nutrients to growing cells. HA with encapsulated primary rat osteoblasts (ROBs) is applied as bio-ink B with a view to improving cell viability, distribution uniformity, and deposition efficiency. The cell-laden PEG–clay constructs not only encapsulated osteoblasts with more than 95% viability in the short term but also exhibited excellent osteogenic ability in the long term, due to the release of bioactive ions (magnesium ions, Mg2+ and silicon ions, Si4+), which induces the suitable microenvironment to promote the differentiation of the loaded exogenous ROBs, both in vitro and in vivo. This 3D-bioprinting method holds much promise for bone tissue regeneration in terms of cell engraftment, survival, and ultimately long-term function.-
dc.languageeng-
dc.publisherWiley Open Access. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)2198-3844-
dc.relation.ispartofAdvanced Science-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subject3D-bioprinting-
dc.subjectNanocomposite hydrogels-
dc.subjectOsteoblast-laden constructs-
dc.subjectOsteogenesis capability-
dc.title3D-Bioprinted Osteoblast-Laden Nanocomposite Hydrogel Constructs with Induced Microenvironments Promote Cell Viability, Differentiation, and Osteogenesis both In Vitro and In Vivo-
dc.typeArticle-
dc.identifier.emailLu, WW: wwlu@hku.hk-
dc.identifier.authorityLu, WW=rp00411-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1002/advs.201700550-
dc.identifier.scopuseid_2-s2.0-85034967440-
dc.identifier.hkuros293520-
dc.identifier.volume5-
dc.identifier.issue3-
dc.identifier.spagearticle no. 1700550, p. 1-
dc.identifier.epage11-
dc.identifier.isiWOS:000428310500002-
dc.publisher.placeGermany-

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